Cytotoxic xanthones isolated from calophyllum depressinervosum and calophyllum buxifolium with antioxidant and cytotoxic activities
The stem bark of Calophyllum depressinervosum and Calophyllum buxifolium were extracted and examined for their antioxidant activities, together with cytotoxicity towards human cancer cells. The methanol extract of C. depressinervosum exhibited good DPPH and NO scavenging effects. The strongest BCB...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English English English |
| Published: |
Elsevier Ltd
2019
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/79802/ http://irep.iium.edu.my/79802/1/79802_Cytotoxic%20xanthones%20isolated%20from%20Calophyllum_article.pdf http://irep.iium.edu.my/79802/2/79802_Cytotoxic%20xanthones%20isolated%20from%20Calophyllum_scopus.pdf http://irep.iium.edu.my/79802/3/79802_Cytotoxic%20xanthones%20isolated%20from%20Calophyllum_wos.pdf |
| Summary: | The stem bark of Calophyllum depressinervosum and Calophyllum buxifolium were extracted and examined for their
antioxidant activities, together with cytotoxicity towards human cancer cells. The methanol extract of C.
depressinervosum exhibited good DPPH and NO scavenging effects. The strongest BCB inhibition and FIC effects
were shown by dichloromethane and ethyl acetate extracts of both species. Overall, DPPH, FRAP and FIC assays
showed strong correlation with TPC. For cytotoxicity, hexane extract of C. depressinervosum possessed the strongest
anti-proliferative activities towards SNU-1 cells while the hexane extract of C. buxifolium showed the strongest activity
towards LS-174T and K562 cells with the IC values ranging from 7 to 17 μg/mL. The purification of plant extracts
afforded eight xanthones, ananixanthone (1), caloxanthone B (2), caloxanthone I (3), caloxanthone J (4) xanthochymone
B (5), thwaitesixanthone (6), 1,3,5,6-tetrahydroxyxanthone (7) and dombakinaxanthone (8). All the xanthones, except 1
were reported for the first time from both Calophyllum species. The xanthones were examined for their cytotoxic effect
against K562 leukemic cells. Compounds 1 and 2 showed strong cytotoxicity with the IC values of 2.96 and 1.23
μg/mL, respectively. The molecular binding interaction of 2 was further investigated by performing molecular docking
study with promising protein receptor Src kinase. |
|---|