Copy number variation of CNVesv27061 analysis among young adults with high blood pressure using optimized droplet digital polymerase chain reaction (ddPCR) method
Several reports and databases on genomic variants have associated variation in DNA sequences (≥ 1kb), or copy number variation (CNV), with susceptibility to common diseases. However, very few reports are found on hypertension and no study has been reported on CNV in prehypertensive and hypertensiv...
| Main Authors: | , , , , |
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| Format: | Proceeding Paper |
| Language: | English English English |
| Published: |
Elsevier Ltd
2019
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/79709/ http://irep.iium.edu.my/79709/20/79709_Copy%20number%20variation%20of%20CNVesv27061_article.pdf http://irep.iium.edu.my/79709/2/79709_Copy%20number%20variation%20of%20CNVesv27061_scopus.pdf http://irep.iium.edu.my/79709/13/79709_Copy%20number%20variation%20of%20CNVesv27061_wos.pdf |
| Summary: | Several reports and databases on genomic variants have associated variation in DNA sequences (≥ 1kb), or copy number
variation (CNV), with susceptibility to common diseases. However, very few reports are found on hypertension and no study has
been reported on CNV in prehypertensive and hypertensive young adult Malaysians. In this comparative cross-sectional study,
133 young adults were recruited, comprising of normotensive (45 subjects), prehypertensive (40 subjects) and mild hypertensive
(48 subjects) subjects. DNA for CNV determination was extracted from 3 ml of blood samples collected. CNV esv27061 was
analysed using optimized droplet digital polymerase chain reaction (ddPCR) method which has enhanced sensitivity and
precision. Frequency distribution patterns of CNV among mild hypertensives showed highest peak copy-number-gain (number of
copies more than 2) particularly in copy numbers 3 and 5. The prehypertensive subjects exhibited marked increase in copy
number 5 when compared with normotensives. All the subjects in this study showed low frequency distribution pattern for copy
numbers 2, 6 and 7. This discovery emphasizes the importance of frequency patterns in determining CNV status of
prehypertensive and mild hypertensive subjects. Optimization method in this study showed that the detection of CNV esv27061
is possible in our sample population. |
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