Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis
Some of the common practices to assess the composition of plant extract, including Carica papaya seed extract (CPSE) are direct injection of the extract, compound separation using polyethylene glycol capillary column (DB-WAX), and non-linear- temperature programming (NLTP) of GC/MS analysis. This st...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Springer Link
2020
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/79437/ http://irep.iium.edu.my/79437/14/79437_Effects%20of%20coated%20capillary%20column%2C%20derivatization%2C%20and%20temperature%20programming.pdf http://irep.iium.edu.my/79437/15/79437_Effects%20of%20coated%20capillary%20column%2C%20derivatization%2C%20and%20temperature%20programming_Scopus.pdf |
| _version_ | 1848788771176185856 |
|---|---|
| author | Abdullah Sani, Muhamad Shirwan Bakar, Jamilah Abdul Rahman, Russly Abas, Faridah |
| author_facet | Abdullah Sani, Muhamad Shirwan Bakar, Jamilah Abdul Rahman, Russly Abas, Faridah |
| author_sort | Abdullah Sani, Muhamad Shirwan |
| building | IIUM Repository |
| collection | Online Access |
| description | Some of the common practices to assess the composition of plant extract, including Carica papaya seed extract (CPSE) are direct injection of the extract, compound separation using polyethylene glycol capillary column (DB-WAX), and non-linear- temperature programming (NLTP) of GC/MS analysis. This study specifically compared the coating of capillary column, sample derivatization, and temperature programming of GC/MS to determine the composition of CPSE. The retention indices (RI) of the detected compounds were determined and compared to the reference RI. In particular, 5% phenyl–95% methylpolysiloxane (HP-5MS)-, DB-WAX-, and biscyanopropyl polysiloxane (HP-88)-coated capillary columns were used to identify the composition of CPSE. For this study, HP-5MS column, which separated the highest number of compounds (26 compounds) from CPSE, was deemed as the most suitable column. The GC/MS analysis of derivatized CPSE identified 21 compound groups, where fatty acids and fatty acid methyl esters served as the major compounds (80.23%), followed by these compounds in decreasing order: amides > nitriles > sterols > fatty aldehydes > organic acids. A stronger correlation determination between the carbon number and alkane retention time of linear-temperature programming (LTP) (R2 = 0.9859) was found, as compared to its correlation determination with NLTP (R2 = 0.9175), which exhibited an almost equal RI of LTP to the reference RI. Conclusively, GC/MS analysis for the derivatized CPSE using HP-5MS column separation and LTP is highly recommended. |
| first_indexed | 2025-11-14T17:46:07Z |
| format | Article |
| id | iium-79437 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:46:07Z |
| publishDate | 2020 |
| publisher | Springer Link |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-794372020-11-25T07:36:12Z http://irep.iium.edu.my/79437/ Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis Abdullah Sani, Muhamad Shirwan Bakar, Jamilah Abdul Rahman, Russly Abas, Faridah QD Chemistry Some of the common practices to assess the composition of plant extract, including Carica papaya seed extract (CPSE) are direct injection of the extract, compound separation using polyethylene glycol capillary column (DB-WAX), and non-linear- temperature programming (NLTP) of GC/MS analysis. This study specifically compared the coating of capillary column, sample derivatization, and temperature programming of GC/MS to determine the composition of CPSE. The retention indices (RI) of the detected compounds were determined and compared to the reference RI. In particular, 5% phenyl–95% methylpolysiloxane (HP-5MS)-, DB-WAX-, and biscyanopropyl polysiloxane (HP-88)-coated capillary columns were used to identify the composition of CPSE. For this study, HP-5MS column, which separated the highest number of compounds (26 compounds) from CPSE, was deemed as the most suitable column. The GC/MS analysis of derivatized CPSE identified 21 compound groups, where fatty acids and fatty acid methyl esters served as the major compounds (80.23%), followed by these compounds in decreasing order: amides > nitriles > sterols > fatty aldehydes > organic acids. A stronger correlation determination between the carbon number and alkane retention time of linear-temperature programming (LTP) (R2 = 0.9859) was found, as compared to its correlation determination with NLTP (R2 = 0.9175), which exhibited an almost equal RI of LTP to the reference RI. Conclusively, GC/MS analysis for the derivatized CPSE using HP-5MS column separation and LTP is highly recommended. Springer Link 2020-03-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/79437/14/79437_Effects%20of%20coated%20capillary%20column%2C%20derivatization%2C%20and%20temperature%20programming.pdf application/pdf en http://irep.iium.edu.my/79437/15/79437_Effects%20of%20coated%20capillary%20column%2C%20derivatization%2C%20and%20temperature%20programming_Scopus.pdf Abdullah Sani, Muhamad Shirwan and Bakar, Jamilah and Abdul Rahman, Russly and Abas, Faridah (2020) Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis. Journal of Analysis and Testing. pp. 1-12. ISSN 2096-241X E-ISSN 2509-4696 https://link.springer.com/article/10.1007/s41664-020-00118-z 10.1007/s41664-020-00118-z |
| spellingShingle | QD Chemistry Abdullah Sani, Muhamad Shirwan Bakar, Jamilah Abdul Rahman, Russly Abas, Faridah Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis |
| title | Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis |
| title_full | Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis |
| title_fullStr | Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis |
| title_full_unstemmed | Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis |
| title_short | Effects of coated capillary column, derivatization, and temperature programming on the identification of Carica papaya seed extract composition using GC/MS analysis |
| title_sort | effects of coated capillary column, derivatization, and temperature programming on the identification of carica papaya seed extract composition using gc/ms analysis |
| topic | QD Chemistry |
| url | http://irep.iium.edu.my/79437/ http://irep.iium.edu.my/79437/ http://irep.iium.edu.my/79437/ http://irep.iium.edu.my/79437/14/79437_Effects%20of%20coated%20capillary%20column%2C%20derivatization%2C%20and%20temperature%20programming.pdf http://irep.iium.edu.my/79437/15/79437_Effects%20of%20coated%20capillary%20column%2C%20derivatization%2C%20and%20temperature%20programming_Scopus.pdf |