The use of FTIR and Raman spectroscopy in combination with chemometrics for analysis of biomolecules in biomedical fluids: a review
Fourier transform infrared (FTIR) and Raman spectroscopy are complementary techniques, typically called vibrational spectroscopy. Both techniques allow simple, rapid, non-destructive, specific, providing fingerprint spectra, and real-time analytical method for analysis of molecules in different sta...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
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IOS Press
2019
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| Online Access: | http://irep.iium.edu.my/79421/ http://irep.iium.edu.my/79421/1/79421_The%20use%20of%20FTIR%20and%20Raman%20spectroscopy_article.pdf http://irep.iium.edu.my/79421/2/79421_The%20use%20of%20FTIR%20and%20Raman%20spectroscopy_wos.pdf |
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| author | ,, Abdul Rohman Windarsih, Anjar Lukitaningsih, Endang ,, Mohamad Rafi Muflih, Betania Kartika Ahmad Fadzillah, Nurrulhidayah |
| author_facet | ,, Abdul Rohman Windarsih, Anjar Lukitaningsih, Endang ,, Mohamad Rafi Muflih, Betania Kartika Ahmad Fadzillah, Nurrulhidayah |
| author_sort | ,, Abdul Rohman |
| building | IIUM Repository |
| collection | Online Access |
| description | Fourier transform infrared (FTIR) and Raman spectroscopy are complementary techniques, typically called vibrational spectroscopy. Both techniques allow simple, rapid, non-destructive, specific, providing fingerprint spectra, and real-time
analytical method for analysis of molecules in different states. Besides, these methods are simple without any excessive sample
pre-treatment, therefore, they are sometimes called as “green analytical methods”. Biofluids have several biomolecules such as
lipid, protein, nucleic acids, and carbohydrates. These biomolecules can be used as biomarkers to detect some types of diseases,
since biomolecules are in direct contact with the human organs. FTIR and Raman spectra of biofluids are complex in nature,
therefore sophisticated statistical techniques, known as chemometrics, must be used to solve the analytical problems related to
quantitative analysis purposes. The objective of this review is to show the capability of FTIR and Raman spectroscopic techniques in combination with chemometrics techniques to analyze the biomolecules in biofluids through an extensive literature
review. During performing this review, several databases in Science citation index, Scopus PubMed, and Google Scholar related
to the topics are identified and downloaded. With the present review, it is known that FTIR and Raman techniques are rapid
method for screening certain diseases by identifying the level changes of some biomolecules. In the future, this method will be
widely used for clinicians as new diagnostic tools for many diseases. |
| first_indexed | 2025-11-14T17:46:05Z |
| format | Article |
| id | iium-79421 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:46:05Z |
| publishDate | 2019 |
| publisher | IOS Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-794212020-03-05T02:06:36Z http://irep.iium.edu.my/79421/ The use of FTIR and Raman spectroscopy in combination with chemometrics for analysis of biomolecules in biomedical fluids: a review ,, Abdul Rohman Windarsih, Anjar Lukitaningsih, Endang ,, Mohamad Rafi Muflih, Betania Kartika Ahmad Fadzillah, Nurrulhidayah Q Science (General) QD Chemistry QP Physiology Fourier transform infrared (FTIR) and Raman spectroscopy are complementary techniques, typically called vibrational spectroscopy. Both techniques allow simple, rapid, non-destructive, specific, providing fingerprint spectra, and real-time analytical method for analysis of molecules in different states. Besides, these methods are simple without any excessive sample pre-treatment, therefore, they are sometimes called as “green analytical methods”. Biofluids have several biomolecules such as lipid, protein, nucleic acids, and carbohydrates. These biomolecules can be used as biomarkers to detect some types of diseases, since biomolecules are in direct contact with the human organs. FTIR and Raman spectra of biofluids are complex in nature, therefore sophisticated statistical techniques, known as chemometrics, must be used to solve the analytical problems related to quantitative analysis purposes. The objective of this review is to show the capability of FTIR and Raman spectroscopic techniques in combination with chemometrics techniques to analyze the biomolecules in biofluids through an extensive literature review. During performing this review, several databases in Science citation index, Scopus PubMed, and Google Scholar related to the topics are identified and downloaded. With the present review, it is known that FTIR and Raman techniques are rapid method for screening certain diseases by identifying the level changes of some biomolecules. In the future, this method will be widely used for clinicians as new diagnostic tools for many diseases. IOS Press 2019 Article PeerReviewed application/pdf en http://irep.iium.edu.my/79421/1/79421_The%20use%20of%20FTIR%20and%20Raman%20spectroscopy_article.pdf application/pdf en http://irep.iium.edu.my/79421/2/79421_The%20use%20of%20FTIR%20and%20Raman%20spectroscopy_wos.pdf ,, Abdul Rohman and Windarsih, Anjar and Lukitaningsih, Endang and ,, Mohamad Rafi and Muflih, Betania Kartika and Ahmad Fadzillah, Nurrulhidayah (2019) The use of FTIR and Raman spectroscopy in combination with chemometrics for analysis of biomolecules in biomedical fluids: a review. Biomedical Spectroscopy and Imaging, 8 (3-4). pp. 55-71. ISSN 2212-8794 https://content-iospress-com.ezproxy.um.edu.my/download/biomedical-spectroscopy-and-imaging/bsi200189?id=biomedical-spectroscopy-and-imaging%2Fbsi200189 10.3233/BSI-200189 |
| spellingShingle | Q Science (General) QD Chemistry QP Physiology ,, Abdul Rohman Windarsih, Anjar Lukitaningsih, Endang ,, Mohamad Rafi Muflih, Betania Kartika Ahmad Fadzillah, Nurrulhidayah The use of FTIR and Raman spectroscopy in combination with chemometrics for analysis of biomolecules in biomedical fluids: a review |
| title | The use of FTIR and Raman spectroscopy in combination with chemometrics for analysis of biomolecules in biomedical fluids: a review |
| title_full | The use of FTIR and Raman spectroscopy in combination with chemometrics for analysis of biomolecules in biomedical fluids: a review |
| title_fullStr | The use of FTIR and Raman spectroscopy in combination with chemometrics for analysis of biomolecules in biomedical fluids: a review |
| title_full_unstemmed | The use of FTIR and Raman spectroscopy in combination with chemometrics for analysis of biomolecules in biomedical fluids: a review |
| title_short | The use of FTIR and Raman spectroscopy in combination with chemometrics for analysis of biomolecules in biomedical fluids: a review |
| title_sort | use of ftir and raman spectroscopy in combination with chemometrics for analysis of biomolecules in biomedical fluids: a review |
| topic | Q Science (General) QD Chemistry QP Physiology |
| url | http://irep.iium.edu.my/79421/ http://irep.iium.edu.my/79421/ http://irep.iium.edu.my/79421/ http://irep.iium.edu.my/79421/1/79421_The%20use%20of%20FTIR%20and%20Raman%20spectroscopy_article.pdf http://irep.iium.edu.my/79421/2/79421_The%20use%20of%20FTIR%20and%20Raman%20spectroscopy_wos.pdf |