Seronegative occult Hepatitis C virus infection (OCI) in a main haemodialysis centre In Pahang, Malaysia
Introduction: Occult HCV infection has a predilection for specific populations such as haemodialysis (HD) patients. The exact natural course, epidemiology, pathogenesis and clinical importance of OCI are unknown. We investigated the existence of OCI among local patients undergoing routine HD at a re...
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| Format: | Article |
| Language: | English English |
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IIUM Press, International Islamic University Malaysia
2019
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| Online Access: | http://irep.iium.edu.my/78061/ http://irep.iium.edu.my/78061/1/78061_Seronegative%20occult%20Hepatitis%20C.pdf http://irep.iium.edu.my/78061/2/78061_Seronegative%20occult%20Hepatitis%20C_WOS.pdf |
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| author | Abdul Rahman, Siti Nurul Fazlin Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen |
| author_facet | Abdul Rahman, Siti Nurul Fazlin Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen |
| author_sort | Abdul Rahman, Siti Nurul Fazlin |
| building | IIUM Repository |
| collection | Online Access |
| description | Introduction: Occult HCV infection has a predilection for specific populations such as haemodialysis (HD) patients. The exact natural course, epidemiology, pathogenesis and clinical importance of OCI are unknown. We investigated the existence of OCI among local patients undergoing routine HD at a referral hospital in Pahang, Malaysia. Methods: Serum and peripheral blood mononuclear cells (PMBCs) were collected from peripheral venous blood samples of seropositive (anti-HCV positive) and seronegative (anti-HCV negative) HD patients as well as healthy individuals (negative control group). Inclusion criteria for the seronegative patients included elevated liver enzymes. Both conventional PCR and strand-specific PCR were used to detect the viral RNA and to indicate active viral replication in PBMCs respectively. Direct DNA sequencing was done to confirm the viral HCV RNA and their genotypes. Results: In the majority (90-100%) of seropositive chronic hepatitis C patients, viral RNA was detected in both serum and PMBCs . Meanwhile, out of 22 seronegative patients, 6 (27%) showed active viral replication in PBMCs but no detectable viral RNA presence in the serum. None of the negative control group had detectable viral RNA. All seronegative patients with OCI were infected with HCV genotype 3 and two of them (2/6) had a slight elevation of their liver enzymes. Conclusion: Seronegative OCI does exist among local hemodialysis patients, with normal or persistently abnormal liver enzyme values. Further investigation is needed to study the mode of viral transmission and clinical significance of OCI in HD setting. |
| first_indexed | 2025-11-14T17:42:33Z |
| format | Article |
| id | iium-78061 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:42:33Z |
| publishDate | 2019 |
| publisher | IIUM Press, International Islamic University Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-780612020-03-31T07:41:15Z http://irep.iium.edu.my/78061/ Seronegative occult Hepatitis C virus infection (OCI) in a main haemodialysis centre In Pahang, Malaysia Abdul Rahman, Siti Nurul Fazlin Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen QR Microbiology QR355 Virology Introduction: Occult HCV infection has a predilection for specific populations such as haemodialysis (HD) patients. The exact natural course, epidemiology, pathogenesis and clinical importance of OCI are unknown. We investigated the existence of OCI among local patients undergoing routine HD at a referral hospital in Pahang, Malaysia. Methods: Serum and peripheral blood mononuclear cells (PMBCs) were collected from peripheral venous blood samples of seropositive (anti-HCV positive) and seronegative (anti-HCV negative) HD patients as well as healthy individuals (negative control group). Inclusion criteria for the seronegative patients included elevated liver enzymes. Both conventional PCR and strand-specific PCR were used to detect the viral RNA and to indicate active viral replication in PBMCs respectively. Direct DNA sequencing was done to confirm the viral HCV RNA and their genotypes. Results: In the majority (90-100%) of seropositive chronic hepatitis C patients, viral RNA was detected in both serum and PMBCs . Meanwhile, out of 22 seronegative patients, 6 (27%) showed active viral replication in PBMCs but no detectable viral RNA presence in the serum. None of the negative control group had detectable viral RNA. All seronegative patients with OCI were infected with HCV genotype 3 and two of them (2/6) had a slight elevation of their liver enzymes. Conclusion: Seronegative OCI does exist among local hemodialysis patients, with normal or persistently abnormal liver enzyme values. Further investigation is needed to study the mode of viral transmission and clinical significance of OCI in HD setting. IIUM Press, International Islamic University Malaysia 2019-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/78061/1/78061_Seronegative%20occult%20Hepatitis%20C.pdf application/pdf en http://irep.iium.edu.my/78061/2/78061_Seronegative%20occult%20Hepatitis%20C_WOS.pdf Abdul Rahman, Siti Nurul Fazlin and Hamzah, Hairul Aini and Mustafa Mahmoud, Mohammed Imad Al-Deen (2019) Seronegative occult Hepatitis C virus infection (OCI) in a main haemodialysis centre In Pahang, Malaysia. IIUM Medical Journal Malaysia, 18 (3). pp. 11-19. E-ISSN 1823-4631 http://iiumedic.net/imjm/v1/download/volume_18_no_3/Vol18No3-011-019.pdf |
| spellingShingle | QR Microbiology QR355 Virology Abdul Rahman, Siti Nurul Fazlin Hamzah, Hairul Aini Mustafa Mahmoud, Mohammed Imad Al-Deen Seronegative occult Hepatitis C virus infection (OCI) in a main haemodialysis centre In Pahang, Malaysia |
| title | Seronegative occult Hepatitis C virus infection (OCI) in a main haemodialysis centre In Pahang, Malaysia |
| title_full | Seronegative occult Hepatitis C virus infection (OCI) in a main haemodialysis centre In Pahang, Malaysia |
| title_fullStr | Seronegative occult Hepatitis C virus infection (OCI) in a main haemodialysis centre In Pahang, Malaysia |
| title_full_unstemmed | Seronegative occult Hepatitis C virus infection (OCI) in a main haemodialysis centre In Pahang, Malaysia |
| title_short | Seronegative occult Hepatitis C virus infection (OCI) in a main haemodialysis centre In Pahang, Malaysia |
| title_sort | seronegative occult hepatitis c virus infection (oci) in a main haemodialysis centre in pahang, malaysia |
| topic | QR Microbiology QR355 Virology |
| url | http://irep.iium.edu.my/78061/ http://irep.iium.edu.my/78061/ http://irep.iium.edu.my/78061/1/78061_Seronegative%20occult%20Hepatitis%20C.pdf http://irep.iium.edu.my/78061/2/78061_Seronegative%20occult%20Hepatitis%20C_WOS.pdf |