Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species
Thirty microsatellite markers developed for River catfish (Hemibagrus nemurus) were screened for cross amplification in six species of catfish. Out of 30 primers, only 3 (10) to 6 (20) produced successful amplifications in six catfish species. Six different loci...
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| Format: | Article |
| Language: | English |
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The Japanese Society of Fish Genetics and Breeding Science
2015
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| Online Access: | http://irep.iium.edu.my/76559/ http://irep.iium.edu.my/76559/1/Cross-Species_Amplification_of_Microsatellite_DNA_.pdf |
| _version_ | 1848788301619658752 |
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| author | Fitri, Muhammad Siraj, Siti Shapor Nakajima, Masamichi |
| author_facet | Fitri, Muhammad Siraj, Siti Shapor Nakajima, Masamichi |
| author_sort | Fitri, Muhammad |
| building | IIUM Repository |
| collection | Online Access |
| description | Thirty microsatellite markers developed for River catfish (Hemibagrus nemurus) were screened for cross amplification in six species of catfish. Out of 30 primers, only 3 (10) to 6 (20) produced successful amplifications in six catfish species. Six different loci could be amplified in Pangasius pangasius and Clarias batrachus, 5 loci in Clarias gariepinus, 4 loci in Pseudomystus siamensis and 3 loci in Clarias macrocephalus and Pangasius nasutus. The low rate of amplification in these 6 closely related species proved the primers are locus-specific. Sequence analysis of locus MnRm7-1 showed differences in the flanking region and repeat motif in certain species, suggesting the existence of indel mutations in catfish species in that particular region. The examined DNA sequence revealed that the repeat motif is conser ved within Pangasiid catfishes and not conser ved within Clariid catfishes |
| first_indexed | 2025-11-14T17:38:39Z |
| format | Article |
| id | iium-76559 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T17:38:39Z |
| publishDate | 2015 |
| publisher | The Japanese Society of Fish Genetics and Breeding Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-765592019-12-26T08:35:10Z http://irep.iium.edu.my/76559/ Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species Fitri, Muhammad Siraj, Siti Shapor Nakajima, Masamichi SH Aquaculture. Fisheries. Angling SH151 Aquaculture - Fish Culture Thirty microsatellite markers developed for River catfish (Hemibagrus nemurus) were screened for cross amplification in six species of catfish. Out of 30 primers, only 3 (10) to 6 (20) produced successful amplifications in six catfish species. Six different loci could be amplified in Pangasius pangasius and Clarias batrachus, 5 loci in Clarias gariepinus, 4 loci in Pseudomystus siamensis and 3 loci in Clarias macrocephalus and Pangasius nasutus. The low rate of amplification in these 6 closely related species proved the primers are locus-specific. Sequence analysis of locus MnRm7-1 showed differences in the flanking region and repeat motif in certain species, suggesting the existence of indel mutations in catfish species in that particular region. The examined DNA sequence revealed that the repeat motif is conser ved within Pangasiid catfishes and not conser ved within Clariid catfishes The Japanese Society of Fish Genetics and Breeding Science 2015 Article PeerReviewed application/pdf en cc_public_domain http://irep.iium.edu.my/76559/1/Cross-Species_Amplification_of_Microsatellite_DNA_.pdf Fitri, Muhammad and Siraj, Siti Shapor and Nakajima, Masamichi (2015) Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species. Fish Genetics and Breeding Science, 43 (2). pp. 49-57. ISSN 1343-7917 http://www.suisan-ikushu.jp/ |
| spellingShingle | SH Aquaculture. Fisheries. Angling SH151 Aquaculture - Fish Culture Fitri, Muhammad Siraj, Siti Shapor Nakajima, Masamichi Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species |
| title | Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species |
| title_full | Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species |
| title_fullStr | Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species |
| title_full_unstemmed | Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species |
| title_short | Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species |
| title_sort | cross-species amplification of microsatellite dna markers and comparison on the sequence of microsatellite regions among southeast asian catfish species |
| topic | SH Aquaculture. Fisheries. Angling SH151 Aquaculture - Fish Culture |
| url | http://irep.iium.edu.my/76559/ http://irep.iium.edu.my/76559/ http://irep.iium.edu.my/76559/1/Cross-Species_Amplification_of_Microsatellite_DNA_.pdf |