Structural integrity of aortic scaffolds decellularized by sonication decellularization system
Sonication decellularization technique has shown effectiveness to remove all the cellular components by the disruption of the cell membranes and removal of the cell debris to prepare the bioscaffolds. However, it is important to confirm whether this technique does not have a detrimental effect o...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English English |
| Published: |
Institute of Electrical and Electronics Engineers Inc.
2019
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/76211/ http://irep.iium.edu.my/76211/1/76211_Structural%20Integrity%20of%20Aortic_scopus.pdf http://irep.iium.edu.my/76211/2/76211_Structural%20Integrity%20of%20Aortic_complete.pdf |
| _version_ | 1848788249362825216 |
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| author | , Aqilah Hazwani Sha'ban, Munirah Azhim, Azran |
| author_facet | , Aqilah Hazwani Sha'ban, Munirah Azhim, Azran |
| author_sort | , Aqilah Hazwani |
| building | IIUM Repository |
| collection | Online Access |
| description | Sonication decellularization technique has shown
effectiveness to remove all the cellular components by the
disruption of the cell membranes and removal of the cell debris
to prepare the bioscaffolds. However, it is important to confirm
whether this technique does not have a detrimental effect on
elastin and collagen in bioscaffolds. The objectives of this study
are to evaluate the structural integrity of bioscaffolds using
histological staining and quantitatively collagen and elastin
measurement. Aortic tissues were sonicated in 0.1% SDS for 10
hours at the frequency of 170 kHz with the power output of
15W and washed in Phosphate Buffer Solution (PBS) for 5
days. Then the sonicated aortic tissues were evaluated by
Hematoxylin & Eosin (H&E) staining for cell removal analysis,
Verhoeff-van Gieson (VVG) staining for visualizing elastin and
Picrosirius Red (PSR) staining for visualizing collagen. The
collagen and elastic fibres were semi-quantified by ImageJ
software. The results showed that sonication decellularization
system can remove all the cellular components while
maintaining the structural integrity of elastin and collagen on
bioscaffolds. This study indicates that sonication
decellularization system could remove all cellular components
and maintain the structure of the extracellular matrix. |
| first_indexed | 2025-11-14T17:37:49Z |
| format | Proceeding Paper |
| id | iium-76211 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:37:49Z |
| publishDate | 2019 |
| publisher | Institute of Electrical and Electronics Engineers Inc. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-762112022-12-09T03:43:38Z http://irep.iium.edu.my/76211/ Structural integrity of aortic scaffolds decellularized by sonication decellularization system , Aqilah Hazwani Sha'ban, Munirah Azhim, Azran Q Science (General) QR Microbiology R Medicine (General) Sonication decellularization technique has shown effectiveness to remove all the cellular components by the disruption of the cell membranes and removal of the cell debris to prepare the bioscaffolds. However, it is important to confirm whether this technique does not have a detrimental effect on elastin and collagen in bioscaffolds. The objectives of this study are to evaluate the structural integrity of bioscaffolds using histological staining and quantitatively collagen and elastin measurement. Aortic tissues were sonicated in 0.1% SDS for 10 hours at the frequency of 170 kHz with the power output of 15W and washed in Phosphate Buffer Solution (PBS) for 5 days. Then the sonicated aortic tissues were evaluated by Hematoxylin & Eosin (H&E) staining for cell removal analysis, Verhoeff-van Gieson (VVG) staining for visualizing elastin and Picrosirius Red (PSR) staining for visualizing collagen. The collagen and elastic fibres were semi-quantified by ImageJ software. The results showed that sonication decellularization system can remove all the cellular components while maintaining the structural integrity of elastin and collagen on bioscaffolds. This study indicates that sonication decellularization system could remove all cellular components and maintain the structure of the extracellular matrix. Institute of Electrical and Electronics Engineers Inc. 2019-01 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/76211/1/76211_Structural%20Integrity%20of%20Aortic_scopus.pdf application/pdf en http://irep.iium.edu.my/76211/2/76211_Structural%20Integrity%20of%20Aortic_complete.pdf , Aqilah Hazwani and Sha'ban, Munirah and Azhim, Azran (2019) Structural integrity of aortic scaffolds decellularized by sonication decellularization system. In: "2018 IEEE EMBS Conference on Biomedical Engineering and Sciences, IECBES 2018", 3 - 6 December 2018, Borneo Convention Centre KuchingDemak-Isthmus Bridge, Sejingkat Kuching, Sarawak. https://ieeexplore-ieee-org.ezproxy.um.edu.my/stamp/stamp.jsp?tp=&arnumber=8626723 10.1109/IECBES.2018.08626723 |
| spellingShingle | Q Science (General) QR Microbiology R Medicine (General) , Aqilah Hazwani Sha'ban, Munirah Azhim, Azran Structural integrity of aortic scaffolds decellularized by sonication decellularization system |
| title | Structural integrity of aortic scaffolds decellularized by sonication decellularization system |
| title_full | Structural integrity of aortic scaffolds decellularized by sonication decellularization system |
| title_fullStr | Structural integrity of aortic scaffolds decellularized by sonication decellularization system |
| title_full_unstemmed | Structural integrity of aortic scaffolds decellularized by sonication decellularization system |
| title_short | Structural integrity of aortic scaffolds decellularized by sonication decellularization system |
| title_sort | structural integrity of aortic scaffolds decellularized by sonication decellularization system |
| topic | Q Science (General) QR Microbiology R Medicine (General) |
| url | http://irep.iium.edu.my/76211/ http://irep.iium.edu.my/76211/ http://irep.iium.edu.my/76211/ http://irep.iium.edu.my/76211/1/76211_Structural%20Integrity%20of%20Aortic_scopus.pdf http://irep.iium.edu.my/76211/2/76211_Structural%20Integrity%20of%20Aortic_complete.pdf |