Relative characterization of immobilized beta-glucosidase on calcium-alginate and acid functionalized-multiwalled carbon nanotubes peel
Natural polymeric gel such as alginate and carrageenan, and carbon nanomaterials, especially MWCNT and graphene are gaining increasing popularity as immobilization support owing to their biocompatibility. Thus, this study attempts to make a relative characterization of the immobilized β-glucosidase...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English English English |
| Published: |
Penerbit UTM Press
2019
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/76123/ http://irep.iium.edu.my/76123/1/76123_Relative%20characterization%20of%20immobilized.pdf http://irep.iium.edu.my/76123/2/76123_Relative%20characterization%20of%20immobilized_SCOPUS.pdf http://irep.iium.edu.my/76123/13/76123%20Relative%20characterization%20of%20immobilized%20WOS.pdf |
| _version_ | 1848788235287789568 |
|---|---|
| author | Jameel, Ahmad Tariq Maalim, Kauthar Yusuf Yusof, Faridah |
| author_facet | Jameel, Ahmad Tariq Maalim, Kauthar Yusuf Yusof, Faridah |
| author_sort | Jameel, Ahmad Tariq |
| building | IIUM Repository |
| collection | Online Access |
| description | Natural polymeric gel such as alginate and carrageenan, and carbon nanomaterials, especially MWCNT and graphene are gaining increasing popularity as immobilization support owing to their biocompatibility. Thus, this study attempts to make a relative characterization of the immobilized β-glucosidase on alginate and multiwalled carbon nanotubes (MWCNT) to assess their relative merits as biocatalyst. Acid functionalization of MWCNT lead to the formation of carboxyl groups at the MWCNT surface which seemed to have role in the stable attachment of the enzyme on the MWCNT surface. The effectiveness of the immobilized enzyme was evaluated using hydrolysis of p-nitrophenyl-β-D-glucopyranoside to p-nitrophenol. The optimum immobilization conditions were found to be 17 mg MWCNT and 4 h incubation time for MWCNT, and 3.5 wt% sodium alginate solution and 2 h incubation time for the Ca-alginate beads. The immobilization yield on MWCNT was found to be 96% and that for the Ca-alginate beads was 86%. The residual activity of the enzyme at the third cycle of subsequent reuse was 85% for the enzyme-MWCNT and almost 50% for the enzyme-alginate beads. Both, Ca-alginate and MWCNT immobilized enzyme were found to follow Michaelis-Menten kinetics. The kinetic data were best represented by the Langmuir plot. The kinetic constants V max and K m for the MWCNT immobilized enzyme were obtained as 1.324 mM/min and 0.801 mM, respectively, while for the alginate immobilized enzyme, the constants predicted were 1.240 mM/min and 0.524 mM, respectively. Thus MWCNT immobilized β-glucosidase exhibited greater stability in terms of activity and reusability compared to Ca-alginate beads. |
| first_indexed | 2025-11-14T17:37:36Z |
| format | Article |
| id | iium-76123 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:37:36Z |
| publishDate | 2019 |
| publisher | Penerbit UTM Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-761232020-03-25T01:41:23Z http://irep.iium.edu.my/76123/ Relative characterization of immobilized beta-glucosidase on calcium-alginate and acid functionalized-multiwalled carbon nanotubes peel Jameel, Ahmad Tariq Maalim, Kauthar Yusuf Yusof, Faridah TA Engineering (General). Civil engineering (General) Natural polymeric gel such as alginate and carrageenan, and carbon nanomaterials, especially MWCNT and graphene are gaining increasing popularity as immobilization support owing to their biocompatibility. Thus, this study attempts to make a relative characterization of the immobilized β-glucosidase on alginate and multiwalled carbon nanotubes (MWCNT) to assess their relative merits as biocatalyst. Acid functionalization of MWCNT lead to the formation of carboxyl groups at the MWCNT surface which seemed to have role in the stable attachment of the enzyme on the MWCNT surface. The effectiveness of the immobilized enzyme was evaluated using hydrolysis of p-nitrophenyl-β-D-glucopyranoside to p-nitrophenol. The optimum immobilization conditions were found to be 17 mg MWCNT and 4 h incubation time for MWCNT, and 3.5 wt% sodium alginate solution and 2 h incubation time for the Ca-alginate beads. The immobilization yield on MWCNT was found to be 96% and that for the Ca-alginate beads was 86%. The residual activity of the enzyme at the third cycle of subsequent reuse was 85% for the enzyme-MWCNT and almost 50% for the enzyme-alginate beads. Both, Ca-alginate and MWCNT immobilized enzyme were found to follow Michaelis-Menten kinetics. The kinetic data were best represented by the Langmuir plot. The kinetic constants V max and K m for the MWCNT immobilized enzyme were obtained as 1.324 mM/min and 0.801 mM, respectively, while for the alginate immobilized enzyme, the constants predicted were 1.240 mM/min and 0.524 mM, respectively. Thus MWCNT immobilized β-glucosidase exhibited greater stability in terms of activity and reusability compared to Ca-alginate beads. Penerbit UTM Press 2019-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/76123/1/76123_Relative%20characterization%20of%20immobilized.pdf application/pdf en http://irep.iium.edu.my/76123/2/76123_Relative%20characterization%20of%20immobilized_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/76123/13/76123%20Relative%20characterization%20of%20immobilized%20WOS.pdf Jameel, Ahmad Tariq and Maalim, Kauthar Yusuf and Yusof, Faridah (2019) Relative characterization of immobilized beta-glucosidase on calcium-alginate and acid functionalized-multiwalled carbon nanotubes peel. Jurnal Teknologi, 81 (3). pp. 1-10. ISSN 0127-9696 https://jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/13314/6541 10.11113/jt.v81.13314 |
| spellingShingle | TA Engineering (General). Civil engineering (General) Jameel, Ahmad Tariq Maalim, Kauthar Yusuf Yusof, Faridah Relative characterization of immobilized beta-glucosidase on calcium-alginate and acid functionalized-multiwalled carbon nanotubes peel |
| title | Relative characterization of immobilized beta-glucosidase on calcium-alginate and acid functionalized-multiwalled carbon nanotubes peel |
| title_full | Relative characterization of immobilized beta-glucosidase on calcium-alginate and acid functionalized-multiwalled carbon nanotubes peel |
| title_fullStr | Relative characterization of immobilized beta-glucosidase on calcium-alginate and acid functionalized-multiwalled carbon nanotubes peel |
| title_full_unstemmed | Relative characterization of immobilized beta-glucosidase on calcium-alginate and acid functionalized-multiwalled carbon nanotubes peel |
| title_short | Relative characterization of immobilized beta-glucosidase on calcium-alginate and acid functionalized-multiwalled carbon nanotubes peel |
| title_sort | relative characterization of immobilized beta-glucosidase on calcium-alginate and acid functionalized-multiwalled carbon nanotubes peel |
| topic | TA Engineering (General). Civil engineering (General) |
| url | http://irep.iium.edu.my/76123/ http://irep.iium.edu.my/76123/ http://irep.iium.edu.my/76123/ http://irep.iium.edu.my/76123/1/76123_Relative%20characterization%20of%20immobilized.pdf http://irep.iium.edu.my/76123/2/76123_Relative%20characterization%20of%20immobilized_SCOPUS.pdf http://irep.iium.edu.my/76123/13/76123%20Relative%20characterization%20of%20immobilized%20WOS.pdf |