The use of active melioidosis detect tm in early diagnosis of melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nurzahirah
NTRODUCTION: Melioidosis is endemic in Malaysia and an important cause of sepsis. Current gold standard for diagnosis is by culture method,but its long procedure will delay the treatment leading to hospital-related mortality.Thus,a good rapid test is needed to reduce its mortality burden. Recently,...
| Main Authors: | , , , , , , |
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| Format: | Proceeding Paper |
| Language: | English English |
| Published: |
2019
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| Online Access: | http://irep.iium.edu.my/75767/ http://irep.iium.edu.my/75767/7/MRS%20poster.pdf http://irep.iium.edu.my/75767/19/MRS%202019%20Abstract%20Book.pdf |
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| author | Wan Ramli, Wan Nurliyana How, Soon Hin Dzawani, M W.O, Wan Izzah Hafizah A. Kashfi, A.R. N, Noorlina Baharudin, Roesnita |
| author_facet | Wan Ramli, Wan Nurliyana How, Soon Hin Dzawani, M W.O, Wan Izzah Hafizah A. Kashfi, A.R. N, Noorlina Baharudin, Roesnita |
| author_sort | Wan Ramli, Wan Nurliyana |
| building | IIUM Repository |
| collection | Online Access |
| description | NTRODUCTION: Melioidosis is endemic in Malaysia and an important cause of sepsis. Current gold standard for diagnosis is by culture method,but its long procedure will delay the treatment leading to hospital-related mortality.Thus,a good rapid test is needed to reduce its mortality burden. Recently, Active Melioidosis Detect (AMD)have been shown to be useful. OBJECTIVES: (1)To measure the sensitivity and specificity of AMD.(2)To study the sensitivity and specificity of early morning urine AMD compared to spot urine AMD. METHOD : A prospective cross-sectional study of clinically suspected melioidosis patients in HTAA and HSNZ from April until December 2018. Blood and urine samples were tested with AMD. Test results were analysed for sensitivity, specificity,positive predictive value and negative predictive value. RESULTS: A total of 89 patients were included in this study.The mean age is 52 years old, and 56.3% were male gender.64% of patients have diabetes mellitus.11 patients have positive blood culture for Bukholderia pseudomallei, 4 of them were tested positive for AMD.3 of them presented with septic shock (3.4%), however none died.The sensitivity of the AMD was 36.4% ([95% CI 12.4 to 68.4]) and the specificity was 66.7% ( [95% CI 46.0 to 82.8]) in all samples, with positive predictive value of 30.7% and negative predictive value of 72%.Blood samples have lower sensitivity of 9.1% ([95% CI 4.8 to 42.9]) with high specificity of 100% ([95% CI 84.5 to 100]). Urine spot samples have higher sensitivity compared to serum and morning urine, with 36.4% ([95%CI 12.4 to 68.4]) and specificity of 88.9% ([95% CI 69.7 to 97.1]). CONCLUSION: From this pilot study, this test requires further evaluation before incorporating as point of care assay. |
| first_indexed | 2025-11-14T17:36:44Z |
| format | Proceeding Paper |
| id | iium-75767 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:36:44Z |
| publishDate | 2019 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-757672020-01-02T10:01:09Z http://irep.iium.edu.my/75767/ The use of active melioidosis detect tm in early diagnosis of melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nurzahirah Wan Ramli, Wan Nurliyana How, Soon Hin Dzawani, M W.O, Wan Izzah Hafizah A. Kashfi, A.R. N, Noorlina Baharudin, Roesnita RC Internal medicine RC111 Infectious and Parasitic Diseases NTRODUCTION: Melioidosis is endemic in Malaysia and an important cause of sepsis. Current gold standard for diagnosis is by culture method,but its long procedure will delay the treatment leading to hospital-related mortality.Thus,a good rapid test is needed to reduce its mortality burden. Recently, Active Melioidosis Detect (AMD)have been shown to be useful. OBJECTIVES: (1)To measure the sensitivity and specificity of AMD.(2)To study the sensitivity and specificity of early morning urine AMD compared to spot urine AMD. METHOD : A prospective cross-sectional study of clinically suspected melioidosis patients in HTAA and HSNZ from April until December 2018. Blood and urine samples were tested with AMD. Test results were analysed for sensitivity, specificity,positive predictive value and negative predictive value. RESULTS: A total of 89 patients were included in this study.The mean age is 52 years old, and 56.3% were male gender.64% of patients have diabetes mellitus.11 patients have positive blood culture for Bukholderia pseudomallei, 4 of them were tested positive for AMD.3 of them presented with septic shock (3.4%), however none died.The sensitivity of the AMD was 36.4% ([95% CI 12.4 to 68.4]) and the specificity was 66.7% ( [95% CI 46.0 to 82.8]) in all samples, with positive predictive value of 30.7% and negative predictive value of 72%.Blood samples have lower sensitivity of 9.1% ([95% CI 4.8 to 42.9]) with high specificity of 100% ([95% CI 84.5 to 100]). Urine spot samples have higher sensitivity compared to serum and morning urine, with 36.4% ([95%CI 12.4 to 68.4]) and specificity of 88.9% ([95% CI 69.7 to 97.1]). CONCLUSION: From this pilot study, this test requires further evaluation before incorporating as point of care assay. 2019-10-09 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/75767/7/MRS%20poster.pdf application/pdf en http://irep.iium.edu.my/75767/19/MRS%202019%20Abstract%20Book.pdf Wan Ramli, Wan Nurliyana and How, Soon Hin and Dzawani, M and W.O, Wan Izzah Hafizah and A. Kashfi, A.R. and N, Noorlina and Baharudin, Roesnita (2019) The use of active melioidosis detect tm in early diagnosis of melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nurzahirah. In: Medical Research Symposium 2019: Expanding Research Collaboration in Health, 9 October 2019, IIUM Medical Centre, Kuantan Pahang. (Unpublished) |
| spellingShingle | RC Internal medicine RC111 Infectious and Parasitic Diseases Wan Ramli, Wan Nurliyana How, Soon Hin Dzawani, M W.O, Wan Izzah Hafizah A. Kashfi, A.R. N, Noorlina Baharudin, Roesnita The use of active melioidosis detect tm in early diagnosis of melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nurzahirah |
| title | The use of active melioidosis detect tm in early diagnosis of melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nurzahirah |
| title_full | The use of active melioidosis detect tm in early diagnosis of melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nurzahirah |
| title_fullStr | The use of active melioidosis detect tm in early diagnosis of melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nurzahirah |
| title_full_unstemmed | The use of active melioidosis detect tm in early diagnosis of melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nurzahirah |
| title_short | The use of active melioidosis detect tm in early diagnosis of melioidosis in Hospital Tengku Ampuan Afzan and Hospital Sultanah Nurzahirah |
| title_sort | use of active melioidosis detect tm in early diagnosis of melioidosis in hospital tengku ampuan afzan and hospital sultanah nurzahirah |
| topic | RC Internal medicine RC111 Infectious and Parasitic Diseases |
| url | http://irep.iium.edu.my/75767/ http://irep.iium.edu.my/75767/7/MRS%20poster.pdf http://irep.iium.edu.my/75767/19/MRS%202019%20Abstract%20Book.pdf |