Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED)
Introduction: Flaxseed extract promotes bone health and possibly function in inducing bone regeneration process. SHED have been considered as an alternative source of MSC in tissue engineering because of their potential to differentiate into multiple cell-lineages including osteoblasts. Although fla...
| Main Authors: | , , , , , , , |
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| Format: | Proceeding Paper |
| Language: | English English English |
| Published: |
2019
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| Online Access: | http://irep.iium.edu.my/75491/ http://irep.iium.edu.my/75491/1/75491_ICPRP%202019%20%28Cover%29.pdf http://irep.iium.edu.my/75491/2/75491_ICPRP%202019%20%28Program%20Book%29.pdf http://irep.iium.edu.my/75491/3/75491_ICPRP%202019%20%28Poster%29.pdf |
| _version_ | 1848788135885930496 |
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| author | Nordin, Nur Sazwi Mokhtar@Makhtar, Khairani Idah Mustafa Al-Ahmad, Basma Ezzat Lestari, Widya Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Thirumulu Ponnuraj, Kannan Ahmad, Azlina |
| author_facet | Nordin, Nur Sazwi Mokhtar@Makhtar, Khairani Idah Mustafa Al-Ahmad, Basma Ezzat Lestari, Widya Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Thirumulu Ponnuraj, Kannan Ahmad, Azlina |
| author_sort | Nordin, Nur Sazwi |
| building | IIUM Repository |
| collection | Online Access |
| description | Introduction: Flaxseed extract promotes bone health and possibly function in inducing bone regeneration process. SHED have been considered as an alternative source of MSC in tissue engineering because of their potential to differentiate into multiple cell-lineages including osteoblasts. Although flaxseed extract has been shown to affect bone formation process, its effect in inducing stem cell into osteoblast lineage has not been reported. Objective: To determine the effects of flaxseed extract on osteoblast differentiation potential of SHED. Materials and methods: SHED cultured in osteoblast induction media (OIM) was treated with flaxseed extract at 4mg/ml. The RNA was collected and extracted with Total RNA Mini Kit (Geneaid) from cells cultured at day 1, 3, 7, 14 and 21 and subjected to reverse-transcriptase PCR for osteoblast markers (OSX, OCN, DMP1). Alkaline phosphatase activity was determined by ALP assay and Alizarin Red S staining was performed to evaluate calcium deposits in SHED. Results: All osteoblast markers were expressed in all samples analysed. Interestingly, the expression of OSX appeared to be reduced in SHED treated with flaxseed extract. In addition, SHED treated with flaxseed extract resulted in low ALP activity compared to control (p<0.05) (t-test). Calcium deposition was positive in SHED cultured in OIM only. Conclusion: Flaxseed appears to reduce the expression of osteoblast markers, ALP activity and calcium deposition in SHED. This suggests that flaxseed as a potential inhibitor in osteoblast differentiation of SHED. |
| first_indexed | 2025-11-14T17:36:01Z |
| format | Proceeding Paper |
| id | iium-75491 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:36:01Z |
| publishDate | 2019 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-754912019-11-18T04:50:07Z http://irep.iium.edu.my/75491/ Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED) Nordin, Nur Sazwi Mokhtar@Makhtar, Khairani Idah Mustafa Al-Ahmad, Basma Ezzat Lestari, Widya Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Thirumulu Ponnuraj, Kannan Ahmad, Azlina RK Dentistry Introduction: Flaxseed extract promotes bone health and possibly function in inducing bone regeneration process. SHED have been considered as an alternative source of MSC in tissue engineering because of their potential to differentiate into multiple cell-lineages including osteoblasts. Although flaxseed extract has been shown to affect bone formation process, its effect in inducing stem cell into osteoblast lineage has not been reported. Objective: To determine the effects of flaxseed extract on osteoblast differentiation potential of SHED. Materials and methods: SHED cultured in osteoblast induction media (OIM) was treated with flaxseed extract at 4mg/ml. The RNA was collected and extracted with Total RNA Mini Kit (Geneaid) from cells cultured at day 1, 3, 7, 14 and 21 and subjected to reverse-transcriptase PCR for osteoblast markers (OSX, OCN, DMP1). Alkaline phosphatase activity was determined by ALP assay and Alizarin Red S staining was performed to evaluate calcium deposits in SHED. Results: All osteoblast markers were expressed in all samples analysed. Interestingly, the expression of OSX appeared to be reduced in SHED treated with flaxseed extract. In addition, SHED treated with flaxseed extract resulted in low ALP activity compared to control (p<0.05) (t-test). Calcium deposition was positive in SHED cultured in OIM only. Conclusion: Flaxseed appears to reduce the expression of osteoblast markers, ALP activity and calcium deposition in SHED. This suggests that flaxseed as a potential inhibitor in osteoblast differentiation of SHED. 2019-10-19 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/75491/1/75491_ICPRP%202019%20%28Cover%29.pdf application/pdf en http://irep.iium.edu.my/75491/2/75491_ICPRP%202019%20%28Program%20Book%29.pdf application/pdf en http://irep.iium.edu.my/75491/3/75491_ICPRP%202019%20%28Poster%29.pdf Nordin, Nur Sazwi and Mokhtar@Makhtar, Khairani Idah and Mustafa Al-Ahmad, Basma Ezzat and Lestari, Widya and Arief Ichwan, Solachuddin Jauhari and Darnis, Deny Susanti and Thirumulu Ponnuraj, Kannan and Ahmad, Azlina (2019) Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED). In: International Conference on Pharmaceutical Research and Pharmacy Practice cum 14th IIUM-MPS Pharmacy Scientific Conference (ICPRP 2019), 19-20 October 2019, Istana Hotel, Kuala lumpur. (Unpublished) |
| spellingShingle | RK Dentistry Nordin, Nur Sazwi Mokhtar@Makhtar, Khairani Idah Mustafa Al-Ahmad, Basma Ezzat Lestari, Widya Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Thirumulu Ponnuraj, Kannan Ahmad, Azlina Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED) |
| title | Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED) |
| title_full | Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED) |
| title_fullStr | Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED) |
| title_full_unstemmed | Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED) |
| title_short | Effects of flaxseed (Linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (SHED) |
| title_sort | effects of flaxseed (linum usitatissimum) extract on osteoblast differentiation potential of stem cells derived from human exfoliated deciduous teeth (shed) |
| topic | RK Dentistry |
| url | http://irep.iium.edu.my/75491/ http://irep.iium.edu.my/75491/1/75491_ICPRP%202019%20%28Cover%29.pdf http://irep.iium.edu.my/75491/2/75491_ICPRP%202019%20%28Program%20Book%29.pdf http://irep.iium.edu.my/75491/3/75491_ICPRP%202019%20%28Poster%29.pdf |