An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria
The development of treatment strategies for periodontitis that maximise the effectiveness of antibiotics is highly desirable. Azithromycin is proving to be an effective antibiotic for treatment of refractory periodontitis which works by binding to the outer membrane of Gram-negative bacteria and sub...
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| Format: | Proceeding Paper |
| Language: | English English |
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Elsevier
2019
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| Online Access: | http://irep.iium.edu.my/75318/ http://irep.iium.edu.my/75318/7/75318%20An%20investigation%20into%20the%20synergistic.pdf http://irep.iium.edu.my/75318/8/75318%20An%20investigation%20into%20the%20synergistic%20SCOPUS.pdf |
| _version_ | 1848788105267511296 |
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| author | Husain, Juzaily Griffiths, Gareth S. Rawlinson, Andrew Stafford, Graham Douglas, zC.W. Ian |
| author_facet | Husain, Juzaily Griffiths, Gareth S. Rawlinson, Andrew Stafford, Graham Douglas, zC.W. Ian |
| author_sort | Husain, Juzaily |
| building | IIUM Repository |
| collection | Online Access |
| description | The development of treatment strategies for periodontitis that maximise the effectiveness of antibiotics is highly desirable. Azithromycin is proving to be an effective antibiotic for treatment of refractory periodontitis which works by binding to the outer membrane of Gram-negative bacteria and subsequently inhibits protein synthesis. Lactoferrin is a membrane-active host antimicrobial protein and so the objective of this study was to determine whether the effect of azithromycin (AZM) against example
periodontopathogens (Porphyromonas gingivalis and Tannerella forsythia) could be potentiated by lactoferrin. Two strains of P.gingivalis and T. forsythia were exposed to lactoferrin (LF; up to 10 mg/ml) and AZM (up to 5 μg/ml) for 0 – 72 h. The MICs for AZM were established using E-Test strips and by agar diffusion. Susceptibility to LF and LF + AZM was evaluated using diffusion assays, with and without iron depletion. The range of MIC values of AZM for P. gingivalis strains and T. forsythia was 0.16 - 0.63
μg/ml and 0.50 - 0.63 μg/ml, respectively. However, no inhibition was observed with iron saturated lactoferrin at any concentration or under iron depletion conditions nor was any effect observed on the AZM MIC by its presence. P. gingivalis and T. forsythia were inhibited by AZM but were not affected by Lf and there was no synergism between AZM and Lf. |
| first_indexed | 2025-11-14T17:35:32Z |
| format | Proceeding Paper |
| id | iium-75318 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:35:32Z |
| publishDate | 2019 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-753182019-11-15T00:52:33Z http://irep.iium.edu.my/75318/ An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria Husain, Juzaily Griffiths, Gareth S. Rawlinson, Andrew Stafford, Graham Douglas, zC.W. Ian RK Dentistry The development of treatment strategies for periodontitis that maximise the effectiveness of antibiotics is highly desirable. Azithromycin is proving to be an effective antibiotic for treatment of refractory periodontitis which works by binding to the outer membrane of Gram-negative bacteria and subsequently inhibits protein synthesis. Lactoferrin is a membrane-active host antimicrobial protein and so the objective of this study was to determine whether the effect of azithromycin (AZM) against example periodontopathogens (Porphyromonas gingivalis and Tannerella forsythia) could be potentiated by lactoferrin. Two strains of P.gingivalis and T. forsythia were exposed to lactoferrin (LF; up to 10 mg/ml) and AZM (up to 5 μg/ml) for 0 – 72 h. The MICs for AZM were established using E-Test strips and by agar diffusion. Susceptibility to LF and LF + AZM was evaluated using diffusion assays, with and without iron depletion. The range of MIC values of AZM for P. gingivalis strains and T. forsythia was 0.16 - 0.63 μg/ml and 0.50 - 0.63 μg/ml, respectively. However, no inhibition was observed with iron saturated lactoferrin at any concentration or under iron depletion conditions nor was any effect observed on the AZM MIC by its presence. P. gingivalis and T. forsythia were inhibited by AZM but were not affected by Lf and there was no synergism between AZM and Lf. Elsevier 2019 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/75318/7/75318%20An%20investigation%20into%20the%20synergistic.pdf application/pdf en http://irep.iium.edu.my/75318/8/75318%20An%20investigation%20into%20the%20synergistic%20SCOPUS.pdf Husain, Juzaily and Griffiths, Gareth S. and Rawlinson, Andrew and Stafford, Graham and Douglas, zC.W. Ian (2019) An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria. In: Conference on BioMedical and Advanced Materials (Bio-CAM 2017), 28th-29th November 2017, Pulau Langkawi, Kedah. https://www.sciencedirect.com/science/article/pii/S2214785319314786 10.1016/j.matpr.2019.06.129 |
| spellingShingle | RK Dentistry Husain, Juzaily Griffiths, Gareth S. Rawlinson, Andrew Stafford, Graham Douglas, zC.W. Ian An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria |
| title | An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria |
| title_full | An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria |
| title_fullStr | An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria |
| title_full_unstemmed | An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria |
| title_short | An investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria |
| title_sort | investigation into the synergistic relationship between lactoferrin and azithromycin with particular reference to periodontopathic bacteria |
| topic | RK Dentistry |
| url | http://irep.iium.edu.my/75318/ http://irep.iium.edu.my/75318/ http://irep.iium.edu.my/75318/ http://irep.iium.edu.my/75318/7/75318%20An%20investigation%20into%20the%20synergistic.pdf http://irep.iium.edu.my/75318/8/75318%20An%20investigation%20into%20the%20synergistic%20SCOPUS.pdf |