Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans
Candida albicans is one of the common causes for oral candidiasis worldwide. The proliferation of antifungal-resistant C. albicans has become a major concern. This study was carried out to evaluate activated virgin coconut oil (AVCO) and crude extract of virgin coconut oil (VCO) as new antifungal ag...
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2019
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| Online Access: | http://irep.iium.edu.my/75103/ http://irep.iium.edu.my/75103/14/75103_Application%20of%20direct%20fluorescence-based%20live_article.pdf http://irep.iium.edu.my/75103/3/letter%20from%20publisher.pdf http://irep.iium.edu.my/75103/9/75103_Application%20of%20direct%20fluorescence-based%20live%20or%20dead%20staining%20for%20assessment%20of%20antifungal%20activity%20of%20coconut%20oil%20against%20candida%20albicans_Scopus.pdf |
| _version_ | 1848788067453763584 |
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| author | Mukhtar, Nor Izzah Abllah, Zurainie M., Azrul Naim Shahdan, Intan Azura Long, Kamariah Haron, Ummi Aqilah |
| author_facet | Mukhtar, Nor Izzah Abllah, Zurainie M., Azrul Naim Shahdan, Intan Azura Long, Kamariah Haron, Ummi Aqilah |
| author_sort | Mukhtar, Nor Izzah |
| building | IIUM Repository |
| collection | Online Access |
| description | Candida albicans is one of the common causes for oral candidiasis worldwide. The proliferation of antifungal-resistant C. albicans has become a major concern. This study was carried out to evaluate activated virgin coconut oil (AVCO) and crude extract of virgin coconut oil (VCO) as new antifungal agents for treatment of oral candidiasis. C. albicans viability was determined using LIVE/DEAD bacterial viability kit. C. albicans cells were grown in yeast peptone dextrose (YPD) broth overnight. The fungus was treated with AVCO and VCO at the concentration of minimum fungicidal concentration (MFC) of 6.24 mg/mL and incubated for three different time points (1, 2, and 3 h). To evaluate the viability of C. albicans, SYTO 9 and propidium iodide (PI) staining were used and the cells were observed using fluorescence microscopy. C. albicans treated with AVCO showed more dead cells compared to cells treated with VCO. The data indicate that exposure of C. albicans to AVCO was the most inhibitory to its growth (p < 0.01). |
| first_indexed | 2025-11-14T17:34:56Z |
| format | Article |
| id | iium-75103 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:34:56Z |
| publishDate | 2019 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-751032020-04-13T04:58:55Z http://irep.iium.edu.my/75103/ Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans Mukhtar, Nor Izzah Abllah, Zurainie M., Azrul Naim Shahdan, Intan Azura Long, Kamariah Haron, Ummi Aqilah RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works Candida albicans is one of the common causes for oral candidiasis worldwide. The proliferation of antifungal-resistant C. albicans has become a major concern. This study was carried out to evaluate activated virgin coconut oil (AVCO) and crude extract of virgin coconut oil (VCO) as new antifungal agents for treatment of oral candidiasis. C. albicans viability was determined using LIVE/DEAD bacterial viability kit. C. albicans cells were grown in yeast peptone dextrose (YPD) broth overnight. The fungus was treated with AVCO and VCO at the concentration of minimum fungicidal concentration (MFC) of 6.24 mg/mL and incubated for three different time points (1, 2, and 3 h). To evaluate the viability of C. albicans, SYTO 9 and propidium iodide (PI) staining were used and the cells were observed using fluorescence microscopy. C. albicans treated with AVCO showed more dead cells compared to cells treated with VCO. The data indicate that exposure of C. albicans to AVCO was the most inhibitory to its growth (p < 0.01). 2019-08-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/75103/14/75103_Application%20of%20direct%20fluorescence-based%20live_article.pdf application/pdf en http://irep.iium.edu.my/75103/3/letter%20from%20publisher.pdf application/pdf en http://irep.iium.edu.my/75103/9/75103_Application%20of%20direct%20fluorescence-based%20live%20or%20dead%20staining%20for%20assessment%20of%20antifungal%20activity%20of%20coconut%20oil%20against%20candida%20albicans_Scopus.pdf Mukhtar, Nor Izzah and Abllah, Zurainie and M., Azrul Naim and Shahdan, Intan Azura and Long, Kamariah and Haron, Ummi Aqilah (2019) Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans. Malaysian Journal of Analytical Sciences, 23 (5). pp. 1-6. ISSN 1394-2506 (In Press) https://doi.org/10.17576/mjas-2019-2305-zz |
| spellingShingle | RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works Mukhtar, Nor Izzah Abllah, Zurainie M., Azrul Naim Shahdan, Intan Azura Long, Kamariah Haron, Ummi Aqilah Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans |
| title | Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans |
| title_full | Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans |
| title_fullStr | Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans |
| title_full_unstemmed | Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans |
| title_short | Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans |
| title_sort | application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans |
| topic | RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works |
| url | http://irep.iium.edu.my/75103/ http://irep.iium.edu.my/75103/ http://irep.iium.edu.my/75103/14/75103_Application%20of%20direct%20fluorescence-based%20live_article.pdf http://irep.iium.edu.my/75103/3/letter%20from%20publisher.pdf http://irep.iium.edu.my/75103/9/75103_Application%20of%20direct%20fluorescence-based%20live%20or%20dead%20staining%20for%20assessment%20of%20antifungal%20activity%20of%20coconut%20oil%20against%20candida%20albicans_Scopus.pdf |