Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology
Abstract: Dietary polyphenols exist in two forms; extractable polyphenols (EPP) or compounds solubilised by aqueous/organic solvents, and non-extractable polyphenols (NEPP) or compounds remain in the corresponding residues after the extraction. At present, most researchers focus on EEP fractions, wh...
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| Format: | Article |
| Language: | English |
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MDPI
2019
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| Online Access: | http://irep.iium.edu.my/75015/ http://irep.iium.edu.my/75015/1/preprints201907.0055.v1.pdf |
| _version_ | 1848788053378727936 |
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| author | Mahmud, Israa Ali Mirghani, Mohamed Elwathig Saeed Yusof, Faridah Al-Khatib, Ma'an Fahmi Rashid |
| author_facet | Mahmud, Israa Ali Mirghani, Mohamed Elwathig Saeed Yusof, Faridah Al-Khatib, Ma'an Fahmi Rashid |
| author_sort | Mahmud, Israa Ali |
| building | IIUM Repository |
| collection | Online Access |
| description | Abstract: Dietary polyphenols exist in two forms; extractable polyphenols (EPP) or compounds solubilised by aqueous/organic solvents, and non-extractable polyphenols (NEPP) or compounds remain in the corresponding residues after the extraction. At present, most researchers focus on EEP fractions, while NEPP is neglected. Thus, this study aimed to release NEPP from the remaining powder residue of Barhi date palm kernels (BDPK) with acid hydrolysis. The related extraction conditions were determined and optimised using response surface methodology (RSM) for maximisation of NEPP with highest cytotoxic and antioxidant activities. The face-centred central composite design (FCCCD) was used to establish treatments based on three independent variables, namely; extraction temperature, time, and solvent/sample ratio. Under the optimal conditions, the experimental values for DPPH radical-scavenging capacity of NEPP (IC50=57.52µg/mL), and cytotoxicity of NEPP against A549 and HT29 cells were IC50=17.4 µg/mL and 31.4µg/mL, respectively. The experimental values were in agreement with those predicted by RSM models, confirming the suitability of the model employed and the success of RSM for optimisation of the extraction conditions for NEPP from BDPK. These results indicate that NEPP from industrial date fruit waste could be a promising candidate as natural antioxidants with significant antiproliferation effect against A549 and HT29 cancer cells in-vitro. |
| first_indexed | 2025-11-14T17:34:42Z |
| format | Article |
| id | iium-75015 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T17:34:42Z |
| publishDate | 2019 |
| publisher | MDPI |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-750152024-05-02T07:12:10Z http://irep.iium.edu.my/75015/ Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology Mahmud, Israa Ali Mirghani, Mohamed Elwathig Saeed Yusof, Faridah Al-Khatib, Ma'an Fahmi Rashid T Technology (General) Abstract: Dietary polyphenols exist in two forms; extractable polyphenols (EPP) or compounds solubilised by aqueous/organic solvents, and non-extractable polyphenols (NEPP) or compounds remain in the corresponding residues after the extraction. At present, most researchers focus on EEP fractions, while NEPP is neglected. Thus, this study aimed to release NEPP from the remaining powder residue of Barhi date palm kernels (BDPK) with acid hydrolysis. The related extraction conditions were determined and optimised using response surface methodology (RSM) for maximisation of NEPP with highest cytotoxic and antioxidant activities. The face-centred central composite design (FCCCD) was used to establish treatments based on three independent variables, namely; extraction temperature, time, and solvent/sample ratio. Under the optimal conditions, the experimental values for DPPH radical-scavenging capacity of NEPP (IC50=57.52µg/mL), and cytotoxicity of NEPP against A549 and HT29 cells were IC50=17.4 µg/mL and 31.4µg/mL, respectively. The experimental values were in agreement with those predicted by RSM models, confirming the suitability of the model employed and the success of RSM for optimisation of the extraction conditions for NEPP from BDPK. These results indicate that NEPP from industrial date fruit waste could be a promising candidate as natural antioxidants with significant antiproliferation effect against A549 and HT29 cancer cells in-vitro. MDPI 2019-07 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/75015/1/preprints201907.0055.v1.pdf Mahmud, Israa Ali and Mirghani, Mohamed Elwathig Saeed and Yusof, Faridah and Al-Khatib, Ma'an Fahmi Rashid (2019) Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology. Preprints. pp. 1-36. https://www.preprints.org/manuscript/201907.0055/v1 https://www.preprints.org/manuscript/201907.0055/v1 |
| spellingShingle | T Technology (General) Mahmud, Israa Ali Mirghani, Mohamed Elwathig Saeed Yusof, Faridah Al-Khatib, Ma'an Fahmi Rashid Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology |
| title | Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology |
| title_full | Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology |
| title_fullStr | Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology |
| title_full_unstemmed | Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology |
| title_short | Effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology |
| title_sort | effects of time, temperature, and solvent ratio on the extraction of non-extractable polyphenols with anticancer activity of barhi date palm kernels extracts using response surface methodology |
| topic | T Technology (General) |
| url | http://irep.iium.edu.my/75015/ http://irep.iium.edu.my/75015/ http://irep.iium.edu.my/75015/ http://irep.iium.edu.my/75015/1/preprints201907.0055.v1.pdf |