Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method
The present invention relates to a novel method to detect and differentiate different strains of infectious bursal disease virus (IBDV) in a chicken and other bird sample. RNA was obtained from said samples by using a pair of primer (Primer FVVC & RVVC) in a reverse transcriptase-polymerase chai...
| Main Authors: | , , , |
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| Format: | Patent |
| Language: | English |
| Published: |
2010
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/7475/ http://irep.iium.edu.my/7475/1/United_States_Patent__7659066.pdf |
| _version_ | 1848776853300445184 |
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| author | Omar, Abdul Rahman Mohd, Hair-Bejo Ideris, Aini Hamzah, Hairul Aini |
| author_facet | Omar, Abdul Rahman Mohd, Hair-Bejo Ideris, Aini Hamzah, Hairul Aini |
| author_sort | Omar, Abdul Rahman |
| building | IIUM Repository |
| collection | Online Access |
| description | The present invention relates to a novel method to detect and differentiate different strains of infectious bursal disease virus (IBDV) in a chicken and other bird sample. RNA was obtained from said samples by using a pair of primer (Primer FVVC & RVVC) in a reverse transcriptase-polymerase chain reaction. Two different primer combinations (Primer IF & IVIR) and (Primer IF & RCLA) and real-time polymerase chain reaction conditions were designed and optimized for rapid differentiation of very virulent and vaccine strains of IBDV based on detection of signatory threshold cycle (Ct) and melting temperature (Tm) values. |
| first_indexed | 2025-11-14T14:36:41Z |
| format | Patent |
| id | iium-7475 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:36:41Z |
| publishDate | 2010 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-74752011-12-21T01:13:45Z http://irep.iium.edu.my/7475/ Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method Omar, Abdul Rahman Mohd, Hair-Bejo Ideris, Aini Hamzah, Hairul Aini Q Science (General) The present invention relates to a novel method to detect and differentiate different strains of infectious bursal disease virus (IBDV) in a chicken and other bird sample. RNA was obtained from said samples by using a pair of primer (Primer FVVC & RVVC) in a reverse transcriptase-polymerase chain reaction. Two different primer combinations (Primer IF & IVIR) and (Primer IF & RCLA) and real-time polymerase chain reaction conditions were designed and optimized for rapid differentiation of very virulent and vaccine strains of IBDV based on detection of signatory threshold cycle (Ct) and melting temperature (Tm) values. 2010-02-09 Patent NonPeerReviewed application/pdf en http://irep.iium.edu.my/7475/1/United_States_Patent__7659066.pdf Omar, Abdul Rahman and Mohd, Hair-Bejo and Ideris, Aini and Hamzah, Hairul Aini (2010) Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method. 7,659,066. http://patft.uspto.gov/ 7,659,066 |
| spellingShingle | Q Science (General) Omar, Abdul Rahman Mohd, Hair-Bejo Ideris, Aini Hamzah, Hairul Aini Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method |
| title | Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method |
| title_full | Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method |
| title_fullStr | Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method |
| title_full_unstemmed | Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method |
| title_short | Detection and distinguishing infections bursal disease virus (IBDV) strains by molecular biology method |
| title_sort | detection and distinguishing infections bursal disease virus (ibdv) strains by molecular biology method |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/7475/ http://irep.iium.edu.my/7475/ http://irep.iium.edu.my/7475/ http://irep.iium.edu.my/7475/1/United_States_Patent__7659066.pdf |