Observation on antioxidant activity from leaves, callus and suspension culture of Justicia gendarusa
This Antioxidant activity and phenolic compound was found in Justicia gendarussa (JG) via total phenolic content (TPC) and α,α-diphenyl-β-pycrilhydrazil hydrate (DPPH) radical scavenging assays. The assays were applied on aqueous and methanolic extracts of leaves, callus culture and cell suspens...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/7466/ http://irep.iium.edu.my/7466/1/irep.iium.edu.my_7466.pdf |
| _version_ | 1848776850907594752 |
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| author | Amid, Azura Johan, Nurul Najwa Jamal, Parveen Wan Mohd Zain, Wan Norzaidani |
| author_facet | Amid, Azura Johan, Nurul Najwa Jamal, Parveen Wan Mohd Zain, Wan Norzaidani |
| author_sort | Amid, Azura |
| building | IIUM Repository |
| collection | Online Access |
| description | This Antioxidant activity and phenolic compound was
found in Justicia gendarussa (JG) via total phenolic content
(TPC) and α,α-diphenyl-β-pycrilhydrazil hydrate (DPPH) radical
scavenging assays. The assays were applied on aqueous and
methanolic extracts of leaves, callus culture and cell suspension
culture of JG. Callus was induced from green and matured leaves
of JG cultured on Murashige and Skoog (MS) basal medium
supplemented with different concentrations of 2,4-
dichlorophenoxyacetic acid (2,4-D)or1-Naphthaleneacetic acid
(NAA) and benzylaminopurine (BAP). Callus induced on NAA
(1-Naphthaleneacetic acid) showed higher phenolic content and
antioxidant activity compared to the one induced on 2,4-D.
Highest total phenolic content; 88mg GAE/g was observed from
callus culture induced with NAA and BAP while highest
antioxidant activity (lowest IC50; 8.46x10-3mg/mL) was observed
in the cell suspension |
| first_indexed | 2025-11-14T14:36:39Z |
| format | Proceeding Paper |
| id | iium-7466 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:36:39Z |
| publishDate | 2011 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-74662011-12-16T07:48:49Z http://irep.iium.edu.my/7466/ Observation on antioxidant activity from leaves, callus and suspension culture of Justicia gendarusa Amid, Azura Johan, Nurul Najwa Jamal, Parveen Wan Mohd Zain, Wan Norzaidani Q Science (General) This Antioxidant activity and phenolic compound was found in Justicia gendarussa (JG) via total phenolic content (TPC) and α,α-diphenyl-β-pycrilhydrazil hydrate (DPPH) radical scavenging assays. The assays were applied on aqueous and methanolic extracts of leaves, callus culture and cell suspension culture of JG. Callus was induced from green and matured leaves of JG cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of 2,4- dichlorophenoxyacetic acid (2,4-D)or1-Naphthaleneacetic acid (NAA) and benzylaminopurine (BAP). Callus induced on NAA (1-Naphthaleneacetic acid) showed higher phenolic content and antioxidant activity compared to the one induced on 2,4-D. Highest total phenolic content; 88mg GAE/g was observed from callus culture induced with NAA and BAP while highest antioxidant activity (lowest IC50; 8.46x10-3mg/mL) was observed in the cell suspension 2011-05-17 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/7466/1/irep.iium.edu.my_7466.pdf Amid, Azura and Johan, Nurul Najwa and Jamal, Parveen and Wan Mohd Zain, Wan Norzaidani (2011) Observation on antioxidant activity from leaves, callus and suspension culture of Justicia gendarusa. In: International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May, 2011 , Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/ |
| spellingShingle | Q Science (General) Amid, Azura Johan, Nurul Najwa Jamal, Parveen Wan Mohd Zain, Wan Norzaidani Observation on antioxidant activity from leaves, callus and suspension culture of Justicia gendarusa |
| title | Observation on antioxidant activity from leaves, callus and suspension culture of Justicia gendarusa |
| title_full | Observation on antioxidant activity from leaves, callus and suspension culture of Justicia gendarusa |
| title_fullStr | Observation on antioxidant activity from leaves, callus and suspension culture of Justicia gendarusa |
| title_full_unstemmed | Observation on antioxidant activity from leaves, callus and suspension culture of Justicia gendarusa |
| title_short | Observation on antioxidant activity from leaves, callus and suspension culture of Justicia gendarusa |
| title_sort | observation on antioxidant activity from leaves, callus and suspension culture of justicia gendarusa |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/7466/ http://irep.iium.edu.my/7466/ http://irep.iium.edu.my/7466/1/irep.iium.edu.my_7466.pdf |