Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia
A collaborative trial study has been conducted for validation of an extraction method and a subsequent PCR for the detection of transgenic rice sold in Saudi Arabia. The tests were carried out in Saudi Arabia using Real-Time PCR and the positive samples were validated in another lab in Malaysia usi...
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| Format: | Article |
| Language: | English |
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Istanbul University Printing House
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/7440/ http://irep.iium.edu.my/7440/3/2116-6098-1-PB_7440.pdf |
| _version_ | 1848776845739163648 |
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| author | Alaraidh, Ibrahim Amid, Azura Abd-El Aziem, Farouk Ibrahim, Mohamed M. Bazaid, Salih Greiner, Ralf Alghunaim, Abdullah |
| author_facet | Alaraidh, Ibrahim Amid, Azura Abd-El Aziem, Farouk Ibrahim, Mohamed M. Bazaid, Salih Greiner, Ralf Alghunaim, Abdullah |
| author_sort | Alaraidh, Ibrahim |
| building | IIUM Repository |
| collection | Online Access |
| description | A collaborative trial study has been conducted for validation of an extraction method and a subsequent PCR for the detection of transgenic rice sold in Saudi Arabia. The tests were carried out in Saudi Arabia using Real-Time PCR and the positive samples were validated in another lab in Malaysia using PCR and agarose gel visualization. The samples were tested for the existence of the NOS Terminator. A total of 150 samples were tested out of which three samples tested positive as GM-rice which were retested in Malaysia. The presence of GMO rice in Saudi Arabia supports the necessity of developing precise quantitative and qualitative ways for routine analyses and detection of GMO products in the Saudi Arabian market. With the discovery of GM products in the Saudi Arabian market it would be of no surprise that other Middle Eastern nations also knowingly or unknowingly import GM crops. |
| first_indexed | 2025-11-14T14:36:34Z |
| format | Article |
| id | iium-7440 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:36:34Z |
| publishDate | 2011 |
| publisher | Istanbul University Printing House |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-74402012-04-25T01:30:39Z http://irep.iium.edu.my/7440/ Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia Alaraidh, Ibrahim Amid, Azura Abd-El Aziem, Farouk Ibrahim, Mohamed M. Bazaid, Salih Greiner, Ralf Alghunaim, Abdullah SB Plant culture A collaborative trial study has been conducted for validation of an extraction method and a subsequent PCR for the detection of transgenic rice sold in Saudi Arabia. The tests were carried out in Saudi Arabia using Real-Time PCR and the positive samples were validated in another lab in Malaysia using PCR and agarose gel visualization. The samples were tested for the existence of the NOS Terminator. A total of 150 samples were tested out of which three samples tested positive as GM-rice which were retested in Malaysia. The presence of GMO rice in Saudi Arabia supports the necessity of developing precise quantitative and qualitative ways for routine analyses and detection of GMO products in the Saudi Arabian market. With the discovery of GM products in the Saudi Arabian market it would be of no surprise that other Middle Eastern nations also knowingly or unknowingly import GM crops. Istanbul University Printing House 2011 Article PeerReviewed application/pdf en http://irep.iium.edu.my/7440/3/2116-6098-1-PB_7440.pdf Alaraidh, Ibrahim and Amid, Azura and Abd-El Aziem, Farouk and Ibrahim, Mohamed M. and Bazaid, Salih and Greiner, Ralf and Alghunaim, Abdullah (2011) Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia. IUFS Journal of Biology, 70 (1). pp. 11-17. ISSN 1300-7041 http://www.iudergi.com/tr/index.php/fenbiyoloji/article/viewFile/2116/1762 |
| spellingShingle | SB Plant culture Alaraidh, Ibrahim Amid, Azura Abd-El Aziem, Farouk Ibrahim, Mohamed M. Bazaid, Salih Greiner, Ralf Alghunaim, Abdullah Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia |
| title | Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia |
| title_full | Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia |
| title_fullStr | Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia |
| title_full_unstemmed | Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia |
| title_short | Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia |
| title_sort | detection of genetically modified rice: collaborative validation study of a pcr based detection of genetically modified rice oryza sativa commercially available in saudi arabia |
| topic | SB Plant culture |
| url | http://irep.iium.edu.my/7440/ http://irep.iium.edu.my/7440/ http://irep.iium.edu.my/7440/3/2116-6098-1-PB_7440.pdf |