Prevalence and subtype distribution of blastocystis sp. in cattle from Pahang, Malaysia
Blastocystis sp. is a common enteric protozoan parasite found in humans and various types of animals worldwide. Recently, genotypic distribution of Blastocystis sp. were revealed in insects, rodents, avian and mammals, which exposed its potential of transmiting the infection to human. However, very...
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Malaysian Society of Parasitology and Tropical Medicine (MSPTM)
2020
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| Online Access: | http://irep.iium.edu.my/74331/ http://irep.iium.edu.my/74331/13/74331_Prevalence%20and%20subtype%20distribution%20of%20Blastocystis%20sp.%20in_Scopus.pdf http://irep.iium.edu.my/74331/14/74331_Prevalence%20and%20subtype%20distribution%20of%20Blastocystis%20sp.%20in_Scopus.pdf |
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| author | Kamaruddin, Sitie Kasmadiana Mohammad, Mardhiah Mat Yusof, Afzan |
| author_facet | Kamaruddin, Sitie Kasmadiana Mohammad, Mardhiah Mat Yusof, Afzan |
| author_sort | Kamaruddin, Sitie Kasmadiana |
| building | IIUM Repository |
| collection | Online Access |
| description | Blastocystis sp. is a common enteric protozoan parasite found in humans and various types of animals worldwide. Recently, genotypic distribution of Blastocystis sp. were revealed in insects, rodents, avian and mammals, which exposed its potential of transmiting the infection to human. However, very little information on current levels of Blastocystis sp. infection were reported in cattle from Malaysia. Herein, a total of 120 stool samples of cattles were collected. While the potential risk of infection such as age, gender, body score, diarrheic condition of the cattle were noted, and the condition as well as the management of the farms was also recorded. All stool sample were cultured, but 80 samples were selected for PCR sequencing analysis. The cultivation and microscopic examination revealed only 25% of the cattle (30/120) were infected with Blastocystis sp.. But, 43.8% of the cattle (35/80) were found positive upon PCR sequencing. The study also found that age, body score condition, diarrheic condition and certain farm were associated with the infection (p<0.05). Six subtypes (STs) that were discovered during the study were ST10 (21.3%;17/35), ST5 (8.8%;7/35), ST3 (7.5%;6/35), ST1 (2.5%;2/35), ST4 (2.5%;2/35) and ST14 (1.3%;1/35). Thus, moderate infections and variants in the genotypic distributions of the cattle suggest its potential for zoonotic transmission. Therefore, this findings could be helpful for further understanding the parasite, which assist studies of its pathogenicity. |
| first_indexed | 2025-11-14T17:33:03Z |
| format | Article |
| id | iium-74331 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:33:03Z |
| publishDate | 2020 |
| publisher | Malaysian Society of Parasitology and Tropical Medicine (MSPTM) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-743312020-11-11T07:01:52Z http://irep.iium.edu.my/74331/ Prevalence and subtype distribution of blastocystis sp. in cattle from Pahang, Malaysia Kamaruddin, Sitie Kasmadiana Mohammad, Mardhiah Mat Yusof, Afzan Q Science (General) R Medicine (General) Blastocystis sp. is a common enteric protozoan parasite found in humans and various types of animals worldwide. Recently, genotypic distribution of Blastocystis sp. were revealed in insects, rodents, avian and mammals, which exposed its potential of transmiting the infection to human. However, very little information on current levels of Blastocystis sp. infection were reported in cattle from Malaysia. Herein, a total of 120 stool samples of cattles were collected. While the potential risk of infection such as age, gender, body score, diarrheic condition of the cattle were noted, and the condition as well as the management of the farms was also recorded. All stool sample were cultured, but 80 samples were selected for PCR sequencing analysis. The cultivation and microscopic examination revealed only 25% of the cattle (30/120) were infected with Blastocystis sp.. But, 43.8% of the cattle (35/80) were found positive upon PCR sequencing. The study also found that age, body score condition, diarrheic condition and certain farm were associated with the infection (p<0.05). Six subtypes (STs) that were discovered during the study were ST10 (21.3%;17/35), ST5 (8.8%;7/35), ST3 (7.5%;6/35), ST1 (2.5%;2/35), ST4 (2.5%;2/35) and ST14 (1.3%;1/35). Thus, moderate infections and variants in the genotypic distributions of the cattle suggest its potential for zoonotic transmission. Therefore, this findings could be helpful for further understanding the parasite, which assist studies of its pathogenicity. Malaysian Society of Parasitology and Tropical Medicine (MSPTM) 2020-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/74331/13/74331_Prevalence%20and%20subtype%20distribution%20of%20Blastocystis%20sp.%20in_Scopus.pdf application/pdf en http://irep.iium.edu.my/74331/14/74331_Prevalence%20and%20subtype%20distribution%20of%20Blastocystis%20sp.%20in_Scopus.pdf Kamaruddin, Sitie Kasmadiana and Mohammad, Mardhiah and Mat Yusof, Afzan (2020) Prevalence and subtype distribution of blastocystis sp. in cattle from Pahang, Malaysia. Tropical Biomedicine, 37 (1). pp. 127-141. ISSN 0127-5720 E-ISSN 2521-9855 http://msptm.org/files/Vol37No1/127-141-Mohammad-M.pdf |
| spellingShingle | Q Science (General) R Medicine (General) Kamaruddin, Sitie Kasmadiana Mohammad, Mardhiah Mat Yusof, Afzan Prevalence and subtype distribution of blastocystis sp. in cattle from Pahang, Malaysia |
| title | Prevalence and subtype distribution of blastocystis sp. in cattle from Pahang, Malaysia |
| title_full | Prevalence and subtype distribution of blastocystis sp. in cattle from Pahang, Malaysia |
| title_fullStr | Prevalence and subtype distribution of blastocystis sp. in cattle from Pahang, Malaysia |
| title_full_unstemmed | Prevalence and subtype distribution of blastocystis sp. in cattle from Pahang, Malaysia |
| title_short | Prevalence and subtype distribution of blastocystis sp. in cattle from Pahang, Malaysia |
| title_sort | prevalence and subtype distribution of blastocystis sp. in cattle from pahang, malaysia |
| topic | Q Science (General) R Medicine (General) |
| url | http://irep.iium.edu.my/74331/ http://irep.iium.edu.my/74331/ http://irep.iium.edu.my/74331/13/74331_Prevalence%20and%20subtype%20distribution%20of%20Blastocystis%20sp.%20in_Scopus.pdf http://irep.iium.edu.my/74331/14/74331_Prevalence%20and%20subtype%20distribution%20of%20Blastocystis%20sp.%20in_Scopus.pdf |