The effect of dental caries in children on the colonisation of Candida species

Objective: To determine the effect of caries towards colonization of Candida spp. Methods: To assess the colonization of Candida spp. in the oral cavity of paediatric patient, samples were obtained from 30 subjects aged five to six years old from International Islamic University Malaysia (IIUM) Dent...

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Main Authors: Kosnin, Muhammad Hazzim, Che Hussin, Harina Akila, Arshad, Nadiah, Ismail, Ahmad Faisal, Abllah, Zurainie, Arzmi, Mohd Hafiz
Format: Proceeding Paper
Language:English
Published: 2019
Subjects:
Online Access:http://irep.iium.edu.my/73639/
http://irep.iium.edu.my/73639/1/73639_The%20effect%20of%20dental%20caries%20in%20children%20_complete.pdf
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author Kosnin, Muhammad Hazzim
Che Hussin, Harina Akila
Arshad, Nadiah
Ismail, Ahmad Faisal
Abllah, Zurainie
Arzmi, Mohd Hafiz
author_facet Kosnin, Muhammad Hazzim
Che Hussin, Harina Akila
Arshad, Nadiah
Ismail, Ahmad Faisal
Abllah, Zurainie
Arzmi, Mohd Hafiz
author_sort Kosnin, Muhammad Hazzim
building IIUM Repository
collection Online Access
description Objective: To determine the effect of caries towards colonization of Candida spp. Methods: To assess the colonization of Candida spp. in the oral cavity of paediatric patient, samples were obtained from 30 subjects aged five to six years old from International Islamic University Malaysia (IIUM) Dental Clinic and Adik Arif Kindergarten in Kuantan, Pahang, Malaysia. The samples were collected from buccal mucosa, palate and tooth surfaces using sterile swab. In addition, 10 mL of patient’s saliva suspension was also collected. Following that, the samples were inoculated aseptically on CHROMagar and incubated for 24 h to 48 h at 37 ºC. Finally, the colour, morphology and colony forming unit were determined. Results: This study has shown that no C. albicans was isolated from caries-free oral cavity. Meanwhile, 76% of children with caries possessed Candida spp. in their oral cavity with 65% of Candida spp. isolated was from the tooth surface. Only 35% of the total isolates were obtained from soft tissues including palatal and buccal mucosa. In addition, 82% and 67% of the yeast isolated from the tooth surface and buccal mucosa, respectively were C. albicans. Meanwhile, only C. albicans (1.2  0.5 x 105 cfu mL-1) was isolated from saliva of children with caries. Conclusions: Dental caries enhance the colonization of Candida spp. particularly C. albicans in children’s oral cavity.
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format Proceeding Paper
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spelling iium-736392019-09-30T08:11:31Z http://irep.iium.edu.my/73639/ The effect of dental caries in children on the colonisation of Candida species Kosnin, Muhammad Hazzim Che Hussin, Harina Akila Arshad, Nadiah Ismail, Ahmad Faisal Abllah, Zurainie Arzmi, Mohd Hafiz QR Microbiology RK Dentistry Objective: To determine the effect of caries towards colonization of Candida spp. Methods: To assess the colonization of Candida spp. in the oral cavity of paediatric patient, samples were obtained from 30 subjects aged five to six years old from International Islamic University Malaysia (IIUM) Dental Clinic and Adik Arif Kindergarten in Kuantan, Pahang, Malaysia. The samples were collected from buccal mucosa, palate and tooth surfaces using sterile swab. In addition, 10 mL of patient’s saliva suspension was also collected. Following that, the samples were inoculated aseptically on CHROMagar and incubated for 24 h to 48 h at 37 ºC. Finally, the colour, morphology and colony forming unit were determined. Results: This study has shown that no C. albicans was isolated from caries-free oral cavity. Meanwhile, 76% of children with caries possessed Candida spp. in their oral cavity with 65% of Candida spp. isolated was from the tooth surface. Only 35% of the total isolates were obtained from soft tissues including palatal and buccal mucosa. In addition, 82% and 67% of the yeast isolated from the tooth surface and buccal mucosa, respectively were C. albicans. Meanwhile, only C. albicans (1.2  0.5 x 105 cfu mL-1) was isolated from saliva of children with caries. Conclusions: Dental caries enhance the colonization of Candida spp. particularly C. albicans in children’s oral cavity. 2019-03 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/73639/1/73639_The%20effect%20of%20dental%20caries%20in%20children%20_complete.pdf Kosnin, Muhammad Hazzim and Che Hussin, Harina Akila and Arshad, Nadiah and Ismail, Ahmad Faisal and Abllah, Zurainie and Arzmi, Mohd Hafiz (2019) The effect of dental caries in children on the colonisation of Candida species. In: 18th Annual Scientific meeting of International Association for Dental Research (IADR) 2019, 30th March 2019, Shah Alam, Selangor. (Unpublished)
spellingShingle QR Microbiology
RK Dentistry
Kosnin, Muhammad Hazzim
Che Hussin, Harina Akila
Arshad, Nadiah
Ismail, Ahmad Faisal
Abllah, Zurainie
Arzmi, Mohd Hafiz
The effect of dental caries in children on the colonisation of Candida species
title The effect of dental caries in children on the colonisation of Candida species
title_full The effect of dental caries in children on the colonisation of Candida species
title_fullStr The effect of dental caries in children on the colonisation of Candida species
title_full_unstemmed The effect of dental caries in children on the colonisation of Candida species
title_short The effect of dental caries in children on the colonisation of Candida species
title_sort effect of dental caries in children on the colonisation of candida species
topic QR Microbiology
RK Dentistry
url http://irep.iium.edu.my/73639/
http://irep.iium.edu.my/73639/1/73639_The%20effect%20of%20dental%20caries%20in%20children%20_complete.pdf