Histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system
Meniscus plays fundamental roles in the knee mechanisms and functions but injuries happen in meniscus have poor healing ability that requires great interventions. Tissue engineered scaffolds serve as one of the interventions to regenerate the required tissue to treat early degenerative joint disease...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English English English |
| Published: |
Institute of Electrical and Electronics Engineers Inc.
2019
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| Online Access: | http://irep.iium.edu.my/72620/ http://irep.iium.edu.my/72620/1/72620_Histological%20and%20biochemical%20evaluations.pdf http://irep.iium.edu.my/72620/2/72620_Histological%20and%20biochemical%20evaluations_SCOPUS.pdf http://irep.iium.edu.my/72620/3/72620_Histological%20and%20biochemical%20evaluations_WOS.pdf |
| _version_ | 1848787645326426112 |
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| author | Mohd. Yusof, Fatihah Sha'ban, Munirah Azhim, Azran |
| author_facet | Mohd. Yusof, Fatihah Sha'ban, Munirah Azhim, Azran |
| author_sort | Mohd. Yusof, Fatihah |
| building | IIUM Repository |
| collection | Online Access |
| description | Meniscus plays fundamental roles in the knee mechanisms and functions but injuries happen in meniscus have poor healing ability that requires great interventions. Tissue engineered scaffolds serve as one of the interventions to regenerate the required tissue to treat early degenerative joint disease. The purpose of this research is to examine the effectiveness of sonication treatment system in complete cellular components removal with preserved extracellular matrix (ECM) compositions in meniscus tissues through histological and biochemical evaluations. Meniscus tissues were decellularized using sonication treatment system for 10 hours treatment time and continued with extensive washing process. Histological evaluations were based on van Gieson and Picrosirius red staining that portrayed complete cellular components removal and preserved collagen networks distribution within sonicated scaffolds respectively. Biochemical evaluations showed markedly reduction in the residual DNA content for sonicated scaffolds while maintain in collagen content. Lastly, agarose gel electrophoresis showed no visible DNA bands for sonicated scaffolds. Therefore, the results concluded that the prepared bioscaffolds by sonication treatment system managed to reduce the immunogenicity of scaffolds by removing most of the cellular components and successfully retained the properties of the extracellular matrix. Thus, it is a suitable decellularization method to be used in tissue engineering applications. |
| first_indexed | 2025-11-14T17:28:13Z |
| format | Proceeding Paper |
| id | iium-72620 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:28:13Z |
| publishDate | 2019 |
| publisher | Institute of Electrical and Electronics Engineers Inc. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-726202019-11-24T11:17:43Z http://irep.iium.edu.my/72620/ Histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system Mohd. Yusof, Fatihah Sha'ban, Munirah Azhim, Azran R Medicine (General) Meniscus plays fundamental roles in the knee mechanisms and functions but injuries happen in meniscus have poor healing ability that requires great interventions. Tissue engineered scaffolds serve as one of the interventions to regenerate the required tissue to treat early degenerative joint disease. The purpose of this research is to examine the effectiveness of sonication treatment system in complete cellular components removal with preserved extracellular matrix (ECM) compositions in meniscus tissues through histological and biochemical evaluations. Meniscus tissues were decellularized using sonication treatment system for 10 hours treatment time and continued with extensive washing process. Histological evaluations were based on van Gieson and Picrosirius red staining that portrayed complete cellular components removal and preserved collagen networks distribution within sonicated scaffolds respectively. Biochemical evaluations showed markedly reduction in the residual DNA content for sonicated scaffolds while maintain in collagen content. Lastly, agarose gel electrophoresis showed no visible DNA bands for sonicated scaffolds. Therefore, the results concluded that the prepared bioscaffolds by sonication treatment system managed to reduce the immunogenicity of scaffolds by removing most of the cellular components and successfully retained the properties of the extracellular matrix. Thus, it is a suitable decellularization method to be used in tissue engineering applications. Institute of Electrical and Electronics Engineers Inc. 2019-01-24 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/72620/1/72620_Histological%20and%20biochemical%20evaluations.pdf application/pdf en http://irep.iium.edu.my/72620/2/72620_Histological%20and%20biochemical%20evaluations_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/72620/3/72620_Histological%20and%20biochemical%20evaluations_WOS.pdf Mohd. Yusof, Fatihah and Sha'ban, Munirah and Azhim, Azran (2019) Histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system. In: 2018 IEEE EMBS Conference on Biomedical Engineering and Sciences )IECBES 2018), 3rd-6th December 2018, Kuching, Sarawak. https://ieeexplore.ieee.org/document/8626689 10.1109/IECBES.2018.8626689 |
| spellingShingle | R Medicine (General) Mohd. Yusof, Fatihah Sha'ban, Munirah Azhim, Azran Histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system |
| title | Histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system |
| title_full | Histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system |
| title_fullStr | Histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system |
| title_full_unstemmed | Histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system |
| title_short | Histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system |
| title_sort | histological and biochemical evaluations of decellularized meniscus tissues using sonication treatment system |
| topic | R Medicine (General) |
| url | http://irep.iium.edu.my/72620/ http://irep.iium.edu.my/72620/ http://irep.iium.edu.my/72620/ http://irep.iium.edu.my/72620/1/72620_Histological%20and%20biochemical%20evaluations.pdf http://irep.iium.edu.my/72620/2/72620_Histological%20and%20biochemical%20evaluations_SCOPUS.pdf http://irep.iium.edu.my/72620/3/72620_Histological%20and%20biochemical%20evaluations_WOS.pdf |