Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells
Apart from the highly sought agarwood resin primarily for perfumery industry, agarwood or gaharu (Aquilaria spp.) in general has been one of basic components in traditional medicine including Ayurvedic, Traditional Chinese Medicine and other communities in the Asian region. While the resin is form...
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| Format: | Article |
| Language: | English English English |
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Marmara University Press
2019
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| Online Access: | http://irep.iium.edu.my/72088/ http://irep.iium.edu.my/72088/1/72088_Uninfected%20agarwood%20branch.pdf http://irep.iium.edu.my/72088/2/72088_Uninfected%20agarwood%20branch_WOS.pdf http://irep.iium.edu.my/72088/13/72088_Uninfected%20agarwood%20branch%20extract%20possess%20cytotoxic%20and%20inhibitory%20effects_scopus.pdf |
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| author | Abbas, Phirdaous Hashim, Yumi Zuhanis Has-Yun Mohd. Salleh, Hamzah |
| author_facet | Abbas, Phirdaous Hashim, Yumi Zuhanis Has-Yun Mohd. Salleh, Hamzah |
| author_sort | Abbas, Phirdaous |
| building | IIUM Repository |
| collection | Online Access |
| description | Apart from the highly sought agarwood resin primarily for perfumery industry, agarwood or gaharu
(Aquilaria spp.) in general has been one of basic components in traditional medicine including Ayurvedic, Traditional
Chinese Medicine and other communities in the Asian region. While the resin is formed in infected trees, this present
study reported the cytotoxicity and attachment inhibition effects of leaf and branch extracts from uninfected agarwood
tree against breast cancer cells. Qualitative extraction screening process was first conducted to screen for suitable
extraction solvents and parts of plant (leaf or branch). Then, the in vitro anti-cancer screening assays including
cytotoxicity and attachment assays were conducted. Branch sample extracted using ethanol and distilled water resulted
in higher yield and more potent cancer inhibiting effects as compared to other solvents. Crude extract obtained after
drying process using ethanol as solvent yielded 9.47 % g/g (branch) and 13.2 % g/g (leaf); distilled water as solvent
yielded 9.33 % g/g (branch) and 12.8 % g/g (leaf), respectively. However, branch extract exhibited more potent cancerinhibiting effects with IC50 of 23 µg/mL (ethanol) and 38 µg/mL (distilled water) as compared to leaf (no intersection
points in the plot). To this end, it can be concluded that extracts from uninfected agarwood tree (Aquilaria subintegra)
possesses cytotoxic and anti-attachment effects on MCF-7 breast cancer cells with ethanolic branch extract being the
most promising. The screening and selection of extraction solvent and plant type are crucial steps towards cost-effective
extraction and further bioprocessing of bioactive compounds from agarwood tree. |
| first_indexed | 2025-11-14T17:26:52Z |
| format | Article |
| id | iium-72088 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:26:52Z |
| publishDate | 2019 |
| publisher | Marmara University Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-720882019-08-05T03:02:34Z http://irep.iium.edu.my/72088/ Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells Abbas, Phirdaous Hashim, Yumi Zuhanis Has-Yun Mohd. Salleh, Hamzah Q Science (General) TP248.13 Biotechnology Apart from the highly sought agarwood resin primarily for perfumery industry, agarwood or gaharu (Aquilaria spp.) in general has been one of basic components in traditional medicine including Ayurvedic, Traditional Chinese Medicine and other communities in the Asian region. While the resin is formed in infected trees, this present study reported the cytotoxicity and attachment inhibition effects of leaf and branch extracts from uninfected agarwood tree against breast cancer cells. Qualitative extraction screening process was first conducted to screen for suitable extraction solvents and parts of plant (leaf or branch). Then, the in vitro anti-cancer screening assays including cytotoxicity and attachment assays were conducted. Branch sample extracted using ethanol and distilled water resulted in higher yield and more potent cancer inhibiting effects as compared to other solvents. Crude extract obtained after drying process using ethanol as solvent yielded 9.47 % g/g (branch) and 13.2 % g/g (leaf); distilled water as solvent yielded 9.33 % g/g (branch) and 12.8 % g/g (leaf), respectively. However, branch extract exhibited more potent cancerinhibiting effects with IC50 of 23 µg/mL (ethanol) and 38 µg/mL (distilled water) as compared to leaf (no intersection points in the plot). To this end, it can be concluded that extracts from uninfected agarwood tree (Aquilaria subintegra) possesses cytotoxic and anti-attachment effects on MCF-7 breast cancer cells with ethanolic branch extract being the most promising. The screening and selection of extraction solvent and plant type are crucial steps towards cost-effective extraction and further bioprocessing of bioactive compounds from agarwood tree. Marmara University Press 2019 Article PeerReviewed application/pdf en http://irep.iium.edu.my/72088/1/72088_Uninfected%20agarwood%20branch.pdf application/pdf en http://irep.iium.edu.my/72088/2/72088_Uninfected%20agarwood%20branch_WOS.pdf application/pdf en http://irep.iium.edu.my/72088/13/72088_Uninfected%20agarwood%20branch%20extract%20possess%20cytotoxic%20and%20inhibitory%20effects_scopus.pdf Abbas, Phirdaous and Hashim, Yumi Zuhanis Has-Yun and Mohd. Salleh, Hamzah (2019) Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells. Journal of Research in Pharmacy, 23 (1). pp. 120-129. ISSN 2630-6344 http://jrespharm.com/uploads/pdf/pdf_MPJ_637.pdf https://doi.org/10.12991/jrp.2018.116 |
| spellingShingle | Q Science (General) TP248.13 Biotechnology Abbas, Phirdaous Hashim, Yumi Zuhanis Has-Yun Mohd. Salleh, Hamzah Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells |
| title | Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells |
| title_full | Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells |
| title_fullStr | Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells |
| title_full_unstemmed | Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells |
| title_short | Uninfected agarwood branch extract possess cytotoxic and inhibitory effects on MCF-7 breast cancer cells |
| title_sort | uninfected agarwood branch extract possess cytotoxic and inhibitory effects on mcf-7 breast cancer cells |
| topic | Q Science (General) TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/72088/ http://irep.iium.edu.my/72088/ http://irep.iium.edu.my/72088/ http://irep.iium.edu.my/72088/1/72088_Uninfected%20agarwood%20branch.pdf http://irep.iium.edu.my/72088/2/72088_Uninfected%20agarwood%20branch_WOS.pdf http://irep.iium.edu.my/72088/13/72088_Uninfected%20agarwood%20branch%20extract%20possess%20cytotoxic%20and%20inhibitory%20effects_scopus.pdf |