Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system
Context: Muntingia calabura L. (Muntingiaceae) exerts antioxidant and anti-inflammatory activities, thus, it might be a good hepatoprotective agent. Objective: This study investigates the effect of methanol extract of M. calabura leaves (MMCL) on hepatic antioxidant and anti-inflammatory activities...
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| Format: | Article |
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Taylor and Francis
2019
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| Online Access: | http://irep.iium.edu.my/72035/ http://irep.iium.edu.my/72035/7/72035_Methanol%20extract%20of%20Muntingia%20calabura%20leaves%20attenuates%20CCl4_scopus.pdf http://irep.iium.edu.my/72035/13/72035_Methanol%20extract%20of%20Muntingia%20calabura%20leaves%20attenuates_wos.pdf http://irep.iium.edu.my/72035/19/72035_Methanol%20extract%20of%20Muntingia%20calabura%20leaves%20attenuates%20CCl4.pdf |
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| author | Zakaria, Zainul Amiruddin Mahmood, Nor Diyana Omar, Maizatul Hasyima Bakhtiar, M. Taher Basir, Rusliza |
| author_facet | Zakaria, Zainul Amiruddin Mahmood, Nor Diyana Omar, Maizatul Hasyima Bakhtiar, M. Taher Basir, Rusliza |
| author_sort | Zakaria, Zainul Amiruddin |
| building | IIUM Repository |
| collection | Online Access |
| description | Context: Muntingia calabura L. (Muntingiaceae) exerts antioxidant and anti-inflammatory activities, thus, it might be a good hepatoprotective agent.
Objective: This study investigates the effect of methanol extract of M. calabura leaves (MMCL) on hepatic antioxidant and anti-inflammatory activities in CCl4-induced hepatotoxic rat.
Materials and methods: Sprague Dawley rats (n 1⁄4 6) were treated (p.o.) with 10% DMSO (Groups 1 and 2), 50mg/kg N-acetylcysteine (Group 3) or, 50, 250, or 500mg/kg MMCL (Groups 4–6) for 7 consecutive days followed by pretreatment (i.p.) with vehicle (Group 1) or 50% CCl4 in olive oil (v/v) (Groups 2–6) on day 7th. Plasma liver enzymes and hepatic antioxidant enzymes and pro-inflammatory cytokines concen- trations were measured while liver histopathology was examined.
Results: MMCL, at 500 mg/kg, significantly (p < 0.05) ameliorated CCl4-induced hepatotoxicity by decreas- ing the plasma level of alanine transaminase (429.1 versus 168.7U/L) and aspartate transaminase (513.8 versus 438.1 U/L) as well as the tissue level of nitric oxide (62.7 versus 24.1 nmol/g tissue). At 50, 250, or 500mg/kg, MMCL significantly (p<0.05) reduced the tumour necrosis factor a (87.8 versus 32.7pg/mg tissue), interleukin-1b (1474.4 versus 618.3pg/mg tissue), and interleukin-6 (136.7 versus 30.8pg/mg tis- sue) while increased the liver catalase (92.1 versus 114.4 U/g tissue) and superoxide dismutase (3.4 versus 5.5 U/g tissue). Additionally, qualitative phytochemicals analysis showed that MMCL contained gallic acid, ferulic acid, quercetin, and genistein.
Discussion and conclusions: MMCL ability to attenuate CCl4-induced hepatotoxicity could be helpful in the development of hepatoprotective agents with fewer side effects. |
| first_indexed | 2025-11-14T17:26:45Z |
| format | Article |
| id | iium-72035 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:26:45Z |
| publishDate | 2019 |
| publisher | Taylor and Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-720352020-06-11T06:50:01Z http://irep.iium.edu.my/72035/ Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system Zakaria, Zainul Amiruddin Mahmood, Nor Diyana Omar, Maizatul Hasyima Bakhtiar, M. Taher Basir, Rusliza RS192 Materia Medica-Pharmaceutical Technology Context: Muntingia calabura L. (Muntingiaceae) exerts antioxidant and anti-inflammatory activities, thus, it might be a good hepatoprotective agent. Objective: This study investigates the effect of methanol extract of M. calabura leaves (MMCL) on hepatic antioxidant and anti-inflammatory activities in CCl4-induced hepatotoxic rat. Materials and methods: Sprague Dawley rats (n 1⁄4 6) were treated (p.o.) with 10% DMSO (Groups 1 and 2), 50mg/kg N-acetylcysteine (Group 3) or, 50, 250, or 500mg/kg MMCL (Groups 4–6) for 7 consecutive days followed by pretreatment (i.p.) with vehicle (Group 1) or 50% CCl4 in olive oil (v/v) (Groups 2–6) on day 7th. Plasma liver enzymes and hepatic antioxidant enzymes and pro-inflammatory cytokines concen- trations were measured while liver histopathology was examined. Results: MMCL, at 500 mg/kg, significantly (p < 0.05) ameliorated CCl4-induced hepatotoxicity by decreas- ing the plasma level of alanine transaminase (429.1 versus 168.7U/L) and aspartate transaminase (513.8 versus 438.1 U/L) as well as the tissue level of nitric oxide (62.7 versus 24.1 nmol/g tissue). At 50, 250, or 500mg/kg, MMCL significantly (p<0.05) reduced the tumour necrosis factor a (87.8 versus 32.7pg/mg tissue), interleukin-1b (1474.4 versus 618.3pg/mg tissue), and interleukin-6 (136.7 versus 30.8pg/mg tis- sue) while increased the liver catalase (92.1 versus 114.4 U/g tissue) and superoxide dismutase (3.4 versus 5.5 U/g tissue). Additionally, qualitative phytochemicals analysis showed that MMCL contained gallic acid, ferulic acid, quercetin, and genistein. Discussion and conclusions: MMCL ability to attenuate CCl4-induced hepatotoxicity could be helpful in the development of hepatoprotective agents with fewer side effects. Taylor and Francis 2019 Article PeerReviewed application/pdf en http://irep.iium.edu.my/72035/7/72035_Methanol%20extract%20of%20Muntingia%20calabura%20leaves%20attenuates%20CCl4_scopus.pdf application/pdf en http://irep.iium.edu.my/72035/13/72035_Methanol%20extract%20of%20Muntingia%20calabura%20leaves%20attenuates_wos.pdf application/pdf en http://irep.iium.edu.my/72035/19/72035_Methanol%20extract%20of%20Muntingia%20calabura%20leaves%20attenuates%20CCl4.pdf Zakaria, Zainul Amiruddin and Mahmood, Nor Diyana and Omar, Maizatul Hasyima and Bakhtiar, M. Taher and Basir, Rusliza (2019) Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system. Pharmaceutical Biology, 57 (1). pp. 335-344. ISSN 1388-0209 E-ISSN 1744-5116 https://www.tandfonline.com/doi/abs/10.1080/13880209.2019.1606836 10.1080/13880209.2019.1606836 |
| spellingShingle | RS192 Materia Medica-Pharmaceutical Technology Zakaria, Zainul Amiruddin Mahmood, Nor Diyana Omar, Maizatul Hasyima Bakhtiar, M. Taher Basir, Rusliza Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system |
| title | Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence
system |
| title_full | Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence
system |
| title_fullStr | Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence
system |
| title_full_unstemmed | Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence
system |
| title_short | Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence
system |
| title_sort | methanol extract of muntingia calabura leaves attenuates ccl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence
system |
| topic | RS192 Materia Medica-Pharmaceutical Technology |
| url | http://irep.iium.edu.my/72035/ http://irep.iium.edu.my/72035/ http://irep.iium.edu.my/72035/ http://irep.iium.edu.my/72035/7/72035_Methanol%20extract%20of%20Muntingia%20calabura%20leaves%20attenuates%20CCl4_scopus.pdf http://irep.iium.edu.my/72035/13/72035_Methanol%20extract%20of%20Muntingia%20calabura%20leaves%20attenuates_wos.pdf http://irep.iium.edu.my/72035/19/72035_Methanol%20extract%20of%20Muntingia%20calabura%20leaves%20attenuates%20CCl4.pdf |