In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion
Periodontitis, a biofilm infection affecting the gum and teeth, has a high prevalence and has no efficient treatment to date. In this study, doxycycline hyclate, a semi synthetic derivative of tetracycline was used as the active pharmaceutical ingredient (API) to formulate an anti-periodontitis e...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English English |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/71912/ http://irep.iium.edu.my/71912/7/71912%20In-vitro%20Release%20Profile.pdf http://irep.iium.edu.my/71912/1/poster%20IABS%202017%20%281%29.pdf |
| _version_ | 1848787532450365440 |
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| author | Mohd Shafri, Mohd Affendi Mohamed, Farahidah Hamidon, Nurhani Emira Adina, Anugerah Budipratama |
| author_facet | Mohd Shafri, Mohd Affendi Mohamed, Farahidah Hamidon, Nurhani Emira Adina, Anugerah Budipratama |
| author_sort | Mohd Shafri, Mohd Affendi |
| building | IIUM Repository |
| collection | Online Access |
| description | Periodontitis, a biofilm infection affecting the gum and teeth, has a high prevalence
and has no efficient treatment to date. In this study, doxycycline hyclate, a semi
synthetic derivative of tetracycline was used as the active pharmaceutical ingredient
(API) to formulate an anti-periodontitis emulsion. Different amount of lecithin and
hydroxypropylmethylcellulose (HPMC), both acting as surfactants, were used in the
formulations and tested for drug release profile. The in-vitro drug release profile was
conducted using USP Apparatus 1 with PBS of pH 6.8 under sink condition. A
predetermined time point h0was set up to take out the solution from the vessel for
quantification. In order to quantify the in-vitro performance, a method validation was
completed using UV-spectrophotometry following the requirements listed under ICH
Q2 (R1) guidelines. A standard calibration curve with a good linearity, R2= 0.9973 was
established. LOD and LOQ were in the range of 0.009 and 0.027 µg/mL respectively,
whereas the slope of the standard curve was 0.051. The recovery rate for intra-day
was from 98.27 to 101.39%, and from 100.85 to 102.57% for inter-day. The RSD was
1.6. It was found that lecithin-added formulation showsed complete drug release after
5 hours. In contrast, lecithin+HPMC-added formulation gave a total release after 2
hours. Thus, the lecithin-added formulation showed a slower release profile compared
to the lecithin+HPMC-added formulation and may be the formulation of choice for
future testings. |
| first_indexed | 2025-11-14T17:26:26Z |
| format | Proceeding Paper |
| id | iium-71912 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:26:26Z |
| publishDate | 2017 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-719122019-05-02T08:43:41Z http://irep.iium.edu.my/71912/ In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion Mohd Shafri, Mohd Affendi Mohamed, Farahidah Hamidon, Nurhani Emira Adina, Anugerah Budipratama RS Pharmacy and materia medica Periodontitis, a biofilm infection affecting the gum and teeth, has a high prevalence and has no efficient treatment to date. In this study, doxycycline hyclate, a semi synthetic derivative of tetracycline was used as the active pharmaceutical ingredient (API) to formulate an anti-periodontitis emulsion. Different amount of lecithin and hydroxypropylmethylcellulose (HPMC), both acting as surfactants, were used in the formulations and tested for drug release profile. The in-vitro drug release profile was conducted using USP Apparatus 1 with PBS of pH 6.8 under sink condition. A predetermined time point h0was set up to take out the solution from the vessel for quantification. In order to quantify the in-vitro performance, a method validation was completed using UV-spectrophotometry following the requirements listed under ICH Q2 (R1) guidelines. A standard calibration curve with a good linearity, R2= 0.9973 was established. LOD and LOQ were in the range of 0.009 and 0.027 µg/mL respectively, whereas the slope of the standard curve was 0.051. The recovery rate for intra-day was from 98.27 to 101.39%, and from 100.85 to 102.57% for inter-day. The RSD was 1.6. It was found that lecithin-added formulation showsed complete drug release after 5 hours. In contrast, lecithin+HPMC-added formulation gave a total release after 2 hours. Thus, the lecithin-added formulation showed a slower release profile compared to the lecithin+HPMC-added formulation and may be the formulation of choice for future testings. 2017 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/71912/7/71912%20In-vitro%20Release%20Profile.pdf application/pdf en http://irep.iium.edu.my/71912/1/poster%20IABS%202017%20%281%29.pdf Mohd Shafri, Mohd Affendi and Mohamed, Farahidah and Hamidon, Nurhani Emira and Adina, Anugerah Budipratama (2017) In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion. In: 2nd International Anatomical and Biomedical Scientific Conference 2017 (IABS), 1st-2nd August 2017, Serdang, Selangor. (Unpublished) |
| spellingShingle | RS Pharmacy and materia medica Mohd Shafri, Mohd Affendi Mohamed, Farahidah Hamidon, Nurhani Emira Adina, Anugerah Budipratama In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion |
| title | In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion |
| title_full | In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion |
| title_fullStr | In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion |
| title_full_unstemmed | In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion |
| title_short | In-vitro release profile of anti-periodontitis doxycycline hyclate emulsion |
| title_sort | in-vitro release profile of anti-periodontitis doxycycline hyclate emulsion |
| topic | RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/71912/ http://irep.iium.edu.my/71912/7/71912%20In-vitro%20Release%20Profile.pdf http://irep.iium.edu.my/71912/1/poster%20IABS%202017%20%281%29.pdf |