Gene expression of selected apoptotic markers in Human Oral Carcinoma (HSC-3) cell lines trewated with myrmecodia pendans plant extract
Introduction: Myrmecodia pendans (M. pendans), also known as Sarang Semut is an epiphyte with potential as a new therapeutic agent. Recently, we reported that M. pendans induced apoptotic activity in human oral squamous carcinoma (HSC-3) cell line. This study aimed to study how M. pendans extra...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/71884/ http://irep.iium.edu.my/71884/1/71884_Gene%20Expression%20of%20Selected%20Apoptotic.pdf |
| _version_ | 1848787526744014848 |
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| author | Iskandar, Siti Hajar Wan Yusry, Wan Nur Aisyah Lestari, Widya Arief Ichwan, Solachuddin Jauhari |
| author_facet | Iskandar, Siti Hajar Wan Yusry, Wan Nur Aisyah Lestari, Widya Arief Ichwan, Solachuddin Jauhari |
| author_sort | Iskandar, Siti Hajar |
| building | IIUM Repository |
| collection | Online Access |
| description | Introduction: Myrmecodia pendans (M. pendans), also known as Sarang Semut is an epiphyte with
potential as a new therapeutic agent. Recently, we reported that M. pendans induced apoptotic
activity in human oral squamous carcinoma (HSC-3) cell line. This study aimed to study how M.
pendans extract affected apoptotic markers BAX/BCL-2 expression.
Materials and methods: M. pendans was purchased from West Papua, Indonesia. The hypocotyl was
allowed to dry thoroughly, then extracted aqueously. The extract was kept at -80 °C before freeze
drying. The crude extract was used in the experiments. HSC-3 cells were cultured in Dulbecco’s
Modified Essential Medium, then exposed to M. pendans at 2.5, 5.0 and 7.5 mg/ml for 24 and 48 h.
Doxorubicin
(0.005 mg/ml) was used as positive control. BAX and BCL-2 expression was analysed by Quantitative
Real Time Polymerase Chain Reaction.
Results: BAX and BCL-2 expression in HSC-3 cells was affected by M. pendans. At 24 h, highest
levels of BAX and BCL-2 were observed with M. pendans at 2.5 mg/ml, while downregulation was
observed at 5 mg/ml. However, after 48 h, BAX and BCL-2 were downregulated at all concentrations.
The ratio of BAX/ BCL-2 in HSC-3 cells at 24 h showed significant upregulation at all treatment
concentrations, but was downregulated after 48 h.
Conclusion: M. pendans extract induced apoptotic activity in HSC-3 cells. This study suggests that
induction of apoptosis in HSC-3 cell line is regulated via the pro-apoptotic (BAX) and anti-
apoptotic (BCL
-2) pathways. |
| first_indexed | 2025-11-14T17:26:20Z |
| format | Proceeding Paper |
| id | iium-71884 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T17:26:20Z |
| publishDate | 2019 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-718842019-05-02T06:43:30Z http://irep.iium.edu.my/71884/ Gene expression of selected apoptotic markers in Human Oral Carcinoma (HSC-3) cell lines trewated with myrmecodia pendans plant extract Iskandar, Siti Hajar Wan Yusry, Wan Nur Aisyah Lestari, Widya Arief Ichwan, Solachuddin Jauhari RK Dentistry Introduction: Myrmecodia pendans (M. pendans), also known as Sarang Semut is an epiphyte with potential as a new therapeutic agent. Recently, we reported that M. pendans induced apoptotic activity in human oral squamous carcinoma (HSC-3) cell line. This study aimed to study how M. pendans extract affected apoptotic markers BAX/BCL-2 expression. Materials and methods: M. pendans was purchased from West Papua, Indonesia. The hypocotyl was allowed to dry thoroughly, then extracted aqueously. The extract was kept at -80 °C before freeze drying. The crude extract was used in the experiments. HSC-3 cells were cultured in Dulbecco’s Modified Essential Medium, then exposed to M. pendans at 2.5, 5.0 and 7.5 mg/ml for 24 and 48 h. Doxorubicin (0.005 mg/ml) was used as positive control. BAX and BCL-2 expression was analysed by Quantitative Real Time Polymerase Chain Reaction. Results: BAX and BCL-2 expression in HSC-3 cells was affected by M. pendans. At 24 h, highest levels of BAX and BCL-2 were observed with M. pendans at 2.5 mg/ml, while downregulation was observed at 5 mg/ml. However, after 48 h, BAX and BCL-2 were downregulated at all concentrations. The ratio of BAX/ BCL-2 in HSC-3 cells at 24 h showed significant upregulation at all treatment concentrations, but was downregulated after 48 h. Conclusion: M. pendans extract induced apoptotic activity in HSC-3 cells. This study suggests that induction of apoptosis in HSC-3 cell line is regulated via the pro-apoptotic (BAX) and anti- apoptotic (BCL -2) pathways. 2019-02-25 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/71884/1/71884_Gene%20Expression%20of%20Selected%20Apoptotic.pdf Iskandar, Siti Hajar and Wan Yusry, Wan Nur Aisyah and Lestari, Widya and Arief Ichwan, Solachuddin Jauhari (2019) Gene expression of selected apoptotic markers in Human Oral Carcinoma (HSC-3) cell lines trewated with myrmecodia pendans plant extract. In: 8th Dental Students’ Scientific Conference IIUM 2019, 25th February 2019, Kuantan, Pahang. (Unpublished) |
| spellingShingle | RK Dentistry Iskandar, Siti Hajar Wan Yusry, Wan Nur Aisyah Lestari, Widya Arief Ichwan, Solachuddin Jauhari Gene expression of selected apoptotic markers in Human Oral Carcinoma (HSC-3) cell lines trewated with myrmecodia pendans plant extract |
| title | Gene expression of selected apoptotic markers in Human Oral Carcinoma (HSC-3) cell lines trewated with myrmecodia pendans plant extract |
| title_full | Gene expression of selected apoptotic markers in Human Oral Carcinoma (HSC-3) cell lines trewated with myrmecodia pendans plant extract |
| title_fullStr | Gene expression of selected apoptotic markers in Human Oral Carcinoma (HSC-3) cell lines trewated with myrmecodia pendans plant extract |
| title_full_unstemmed | Gene expression of selected apoptotic markers in Human Oral Carcinoma (HSC-3) cell lines trewated with myrmecodia pendans plant extract |
| title_short | Gene expression of selected apoptotic markers in Human Oral Carcinoma (HSC-3) cell lines trewated with myrmecodia pendans plant extract |
| title_sort | gene expression of selected apoptotic markers in human oral carcinoma (hsc-3) cell lines trewated with myrmecodia pendans plant extract |
| topic | RK Dentistry |
| url | http://irep.iium.edu.my/71884/ http://irep.iium.edu.my/71884/1/71884_Gene%20Expression%20of%20Selected%20Apoptotic.pdf |