Anti-proliferative bacteriocin active against MRSA from coagulase negative Ent. mundtdii strain C4L10 isolated from non broiler chicken
Bacteriocins produced by a variety of microbes are gaining more attention for not only as alternative foodpreservative but also as therapeutics agent. A bacteriocin, Ent C4L10, was produced by coagulase negative Enterococcus mundtii strain C4L10 (Accession No. KC731423) previously isolated from Mala...
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| Format: | Proceeding Paper |
| Language: | English |
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Journal of Probiotics & Health
2014
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| Online Access: | http://irep.iium.edu.my/71575/ http://irep.iium.edu.my/71575/6/71575%20%20Anti%20proliferative%20bacteriocin.pdf |
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| author | Tengku Abdul Hamid, Tengku Haziyamin Alhaji Yusuf, Moshood Arief Ichwan, Solachuddin Jauhari |
| author_facet | Tengku Abdul Hamid, Tengku Haziyamin Alhaji Yusuf, Moshood Arief Ichwan, Solachuddin Jauhari |
| author_sort | Tengku Abdul Hamid, Tengku Haziyamin |
| building | IIUM Repository |
| collection | Online Access |
| description | Bacteriocins produced by a variety of microbes are gaining more attention for not only as alternative foodpreservative but also as therapeutics agent. A bacteriocin, Ent C4L10, was produced by coagulase negative Enterococcus mundtii strain C4L10 (Accession No. KC731423) previously isolated from Malaysian non-broiler chicken. Based on agar diffusion assay, it showed antimicrobial activities against Methicillin resistance Staphylococcus aureus (MRSA) used as an indicator organism. Approximately 10 kDa protein was purified employing three-phase partitioning (TPP) method and it was shown to be highly thermostable, retaining activities at 121°C for 15 min, and was stable in a pH range of 4-9. There was however a loss in activity after protease treatment. PCR amplification using enterocin gene primers showed that Ent C4L10 sequence is highly similar to bacteriocinL-1077 (83% identity). In order to study its anti-proliferative potential, purified Ent C4L10 was also tested against four human cell lines; i.e., lung cancer (H1299), breast cancer (MCF 7), colon cancer (HCT116) and oral cancer (HSC3). It was found that oral cancer cell line wasthe most sensitive with a cytotoxic index of IC50 of 9.009 µg/ml, followed by breast cancer IC50 (11.51 µg/ml), and the least sensitive was with colon cancer cell line (IC50 of 20.57 µg/ml). In conclusion, putative 10KDaEnt C4L10 is a class II bacteriocin isolated from coagulase negative Enterococcus mundtii strain C4L10 shown to have anti-proliferative properties. Therefore, this bacteriocin has not only great potential for use in food preservation, its future use as an antitumoragent should also be explored. |
| first_indexed | 2025-11-14T17:25:28Z |
| format | Proceeding Paper |
| id | iium-71575 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T17:25:28Z |
| publishDate | 2014 |
| publisher | Journal of Probiotics & Health |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-715752019-04-12T07:52:57Z http://irep.iium.edu.my/71575/ Anti-proliferative bacteriocin active against MRSA from coagulase negative Ent. mundtdii strain C4L10 isolated from non broiler chicken Tengku Abdul Hamid, Tengku Haziyamin Alhaji Yusuf, Moshood Arief Ichwan, Solachuddin Jauhari Q Science (General) QR Microbiology Bacteriocins produced by a variety of microbes are gaining more attention for not only as alternative foodpreservative but also as therapeutics agent. A bacteriocin, Ent C4L10, was produced by coagulase negative Enterococcus mundtii strain C4L10 (Accession No. KC731423) previously isolated from Malaysian non-broiler chicken. Based on agar diffusion assay, it showed antimicrobial activities against Methicillin resistance Staphylococcus aureus (MRSA) used as an indicator organism. Approximately 10 kDa protein was purified employing three-phase partitioning (TPP) method and it was shown to be highly thermostable, retaining activities at 121°C for 15 min, and was stable in a pH range of 4-9. There was however a loss in activity after protease treatment. PCR amplification using enterocin gene primers showed that Ent C4L10 sequence is highly similar to bacteriocinL-1077 (83% identity). In order to study its anti-proliferative potential, purified Ent C4L10 was also tested against four human cell lines; i.e., lung cancer (H1299), breast cancer (MCF 7), colon cancer (HCT116) and oral cancer (HSC3). It was found that oral cancer cell line wasthe most sensitive with a cytotoxic index of IC50 of 9.009 µg/ml, followed by breast cancer IC50 (11.51 µg/ml), and the least sensitive was with colon cancer cell line (IC50 of 20.57 µg/ml). In conclusion, putative 10KDaEnt C4L10 is a class II bacteriocin isolated from coagulase negative Enterococcus mundtii strain C4L10 shown to have anti-proliferative properties. Therefore, this bacteriocin has not only great potential for use in food preservation, its future use as an antitumoragent should also be explored. Journal of Probiotics & Health 2014 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/71575/6/71575%20%20Anti%20proliferative%20bacteriocin.pdf Tengku Abdul Hamid, Tengku Haziyamin and Alhaji Yusuf, Moshood and Arief Ichwan, Solachuddin Jauhari (2014) Anti-proliferative bacteriocin active against MRSA from coagulase negative Ent. mundtdii strain C4L10 isolated from non broiler chicken. In: 3rd International Conference & Exhibition on Probiotics, Functional & Baby Foods, 23rd-25th September 2014, Naples Italy. https://www.omicsonline.org/proceedings/antiproliferative-bacteriocins-active-against-mrsa-from-coagulase-negative-ent-mundtii-strain-c4l10-isolated-from-nonbroiler-chicken-21288.html 10.4172/2329-8901.S1.015 |
| spellingShingle | Q Science (General) QR Microbiology Tengku Abdul Hamid, Tengku Haziyamin Alhaji Yusuf, Moshood Arief Ichwan, Solachuddin Jauhari Anti-proliferative bacteriocin active against MRSA from coagulase negative Ent. mundtdii strain C4L10 isolated from non broiler chicken |
| title | Anti-proliferative bacteriocin active against MRSA from coagulase negative Ent. mundtdii strain C4L10 isolated from non broiler chicken |
| title_full | Anti-proliferative bacteriocin active against MRSA from coagulase negative Ent. mundtdii strain C4L10 isolated from non broiler chicken |
| title_fullStr | Anti-proliferative bacteriocin active against MRSA from coagulase negative Ent. mundtdii strain C4L10 isolated from non broiler chicken |
| title_full_unstemmed | Anti-proliferative bacteriocin active against MRSA from coagulase negative Ent. mundtdii strain C4L10 isolated from non broiler chicken |
| title_short | Anti-proliferative bacteriocin active against MRSA from coagulase negative Ent. mundtdii strain C4L10 isolated from non broiler chicken |
| title_sort | anti-proliferative bacteriocin active against mrsa from coagulase negative ent. mundtdii strain c4l10 isolated from non broiler chicken |
| topic | Q Science (General) QR Microbiology |
| url | http://irep.iium.edu.my/71575/ http://irep.iium.edu.my/71575/ http://irep.iium.edu.my/71575/ http://irep.iium.edu.my/71575/6/71575%20%20Anti%20proliferative%20bacteriocin.pdf |