Non-alcoholic steatohepatitis animal model induction
Aim: The aims of this study are to establish NASH animal model utilizing 1% and 12% cholesterol diet. Methods: This study begins with the induction phase with cholesterol diet. In the induction phase, 42 Sprague-Dawley rats were randomly divided into standard diet (std) group (n=12) and NASH group (...
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| Format: | Article |
| Language: | English |
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World Journal of Pharmacy and Pharmaceutical Sciences (WJPSS)
2019
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| Online Access: | http://irep.iium.edu.my/71485/ http://irep.iium.edu.my/71485/1/71485_Non-alcoholic%20steatohepatitis%20animal.pdf |
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| author | Abdul Rahim, Roslina Abdul Ghani, Azril Shahreez Muhammad, Siti Aeshah @ Naznin Abdullah, Nor Zamzila Ramli, Norazsida |
| author_facet | Abdul Rahim, Roslina Abdul Ghani, Azril Shahreez Muhammad, Siti Aeshah @ Naznin Abdullah, Nor Zamzila Ramli, Norazsida |
| author_sort | Abdul Rahim, Roslina |
| building | IIUM Repository |
| collection | Online Access |
| description | Aim: The aims of this study are to establish NASH animal model utilizing 1% and 12% cholesterol diet. Methods: This study begins with the induction phase with cholesterol diet. In the induction phase, 42 Sprague-Dawley rats were randomly divided into standard diet (std) group (n=12) and NASH group (n=30). The 12 rats in the std group were fed with commercial rat pellet while the 30 rats in the NASH group were fed with 1% cholesterol (1% CD) diet for 14 weeks. The experiment was extended for an additional 6 weeks with increment of cholesterol in the diet from 1% to 12 % in the NASH group since the liver histology did not show features of NASH in the 1% CD. Results: At the end of the additional 6 weeks period, liver histology showed
NASH changes in the 12% CD. Results of the histological sections of the livers of the 12% CD rats in the induction phase exhibited features consistent with NASH which include macrovesicular steatosis, occasional hepatocyte ballooning degeneration with mild to moderate lobular and portal inflammation. The 12% CD group revealed significantly increased serum alanine aminotransferase (ALT), serum insulin levels, homeostatic model assessment insulin resistance (HOMA-IR), serum total cholesterol (TC), serum low-density lipoprotein cholesterol (LDL-c) and reduced high-density lipoprotein cholesterol (HDL-c) in rats. Conclusion: This study has successfully induced mild–moderate NASH rat model utilizing 12% cholesterol diet. |
| first_indexed | 2025-11-14T17:25:14Z |
| format | Article |
| id | iium-71485 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T17:25:14Z |
| publishDate | 2019 |
| publisher | World Journal of Pharmacy and Pharmaceutical Sciences (WJPSS) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-714852022-04-06T02:07:51Z http://irep.iium.edu.my/71485/ Non-alcoholic steatohepatitis animal model induction Abdul Rahim, Roslina Abdul Ghani, Azril Shahreez Muhammad, Siti Aeshah @ Naznin Abdullah, Nor Zamzila Ramli, Norazsida QP Physiology R Medicine (General) RB Pathology RC Internal medicine RC803 Specialties of Internal Medicine-Diseases of The Digestive System. Gastroenterology Aim: The aims of this study are to establish NASH animal model utilizing 1% and 12% cholesterol diet. Methods: This study begins with the induction phase with cholesterol diet. In the induction phase, 42 Sprague-Dawley rats were randomly divided into standard diet (std) group (n=12) and NASH group (n=30). The 12 rats in the std group were fed with commercial rat pellet while the 30 rats in the NASH group were fed with 1% cholesterol (1% CD) diet for 14 weeks. The experiment was extended for an additional 6 weeks with increment of cholesterol in the diet from 1% to 12 % in the NASH group since the liver histology did not show features of NASH in the 1% CD. Results: At the end of the additional 6 weeks period, liver histology showed NASH changes in the 12% CD. Results of the histological sections of the livers of the 12% CD rats in the induction phase exhibited features consistent with NASH which include macrovesicular steatosis, occasional hepatocyte ballooning degeneration with mild to moderate lobular and portal inflammation. The 12% CD group revealed significantly increased serum alanine aminotransferase (ALT), serum insulin levels, homeostatic model assessment insulin resistance (HOMA-IR), serum total cholesterol (TC), serum low-density lipoprotein cholesterol (LDL-c) and reduced high-density lipoprotein cholesterol (HDL-c) in rats. Conclusion: This study has successfully induced mild–moderate NASH rat model utilizing 12% cholesterol diet. World Journal of Pharmacy and Pharmaceutical Sciences (WJPSS) 2019-04-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/71485/1/71485_Non-alcoholic%20steatohepatitis%20animal.pdf Abdul Rahim, Roslina and Abdul Ghani, Azril Shahreez and Muhammad, Siti Aeshah @ Naznin and Abdullah, Nor Zamzila and Ramli, Norazsida (2019) Non-alcoholic steatohepatitis animal model induction. World Journal of Pharmacy and Pharmaceutical Sciences, 8 (4). pp. 1523-1538. ISSN 2278-4357 http://www.wjpps.com/wjpps_controller/current_issues |
| spellingShingle | QP Physiology R Medicine (General) RB Pathology RC Internal medicine RC803 Specialties of Internal Medicine-Diseases of The Digestive System. Gastroenterology Abdul Rahim, Roslina Abdul Ghani, Azril Shahreez Muhammad, Siti Aeshah @ Naznin Abdullah, Nor Zamzila Ramli, Norazsida Non-alcoholic steatohepatitis animal model induction |
| title | Non-alcoholic steatohepatitis animal model induction |
| title_full | Non-alcoholic steatohepatitis animal model induction |
| title_fullStr | Non-alcoholic steatohepatitis animal model induction |
| title_full_unstemmed | Non-alcoholic steatohepatitis animal model induction |
| title_short | Non-alcoholic steatohepatitis animal model induction |
| title_sort | non-alcoholic steatohepatitis animal model induction |
| topic | QP Physiology R Medicine (General) RB Pathology RC Internal medicine RC803 Specialties of Internal Medicine-Diseases of The Digestive System. Gastroenterology |
| url | http://irep.iium.edu.my/71485/ http://irep.iium.edu.my/71485/ http://irep.iium.edu.my/71485/1/71485_Non-alcoholic%20steatohepatitis%20animal.pdf |