Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes

Species identification of sea cucumbers that have undergone body deformation due to extensive food processing e.g. beche-de-mer is difficult especially with the copresence of cases of unlabelled or mislabelled sea cucumber-based products in the markets. Therefore, a study was done to determine the s...

Full description

Bibliographic Details
Main Authors: Kamarudin, Kamarul Rahim, Mohamed Rehan, Maryam, Mohamed Rehan, Aisyah
Format: Article
Language:English
Published: Faculty of International Maritime Studies, Kasetsart University 2019
Subjects:
Online Access:http://irep.iium.edu.my/70637/
http://irep.iium.edu.my/70637/1/Maritime%20Technology%20and%20Research%202019.pdf
_version_ 1848787324387721216
author Kamarudin, Kamarul Rahim
Mohamed Rehan, Maryam
Mohamed Rehan, Aisyah
author_facet Kamarudin, Kamarul Rahim
Mohamed Rehan, Maryam
Mohamed Rehan, Aisyah
author_sort Kamarudin, Kamarul Rahim
building IIUM Repository
collection Online Access
description Species identification of sea cucumbers that have undergone body deformation due to extensive food processing e.g. beche-de-mer is difficult especially with the copresence of cases of unlabelled or mislabelled sea cucumber-based products in the markets. Therefore, a study was done to determine the species identities of processed sea cucumbers from selected Malaysian markets using concatenated gene sequences of non-protein-coding 12S and 16S mitochondrial rRNA genes. Phylogenetic analyses based on the distance-based Neighbour Joining method, and the character-based methods i.e. the Maximum Parsimony method, Maximum Likelihood method, and the Bayesian Analysis method of 47 ingroup sequences representing 37 processed sea cucumber specimens, 6 reference samples, and 4 additional specimens suggested the presence of 3 main clusters i.e. gamat family consisting of genus Stichopus and genus Thelenota; and timun laut family comprising family Holothuriidae. A number of 3 gamat species i.e. Stichopus horrens, Stichopus vastus, and Thelenota anax were recorded. Meanwhile, the specimens of Holothuria (Halodeima) atra, Holothuria (Halodeima) edulis, Holothuria (Metriatyla) lessoni, Holothuria (Mertensiothuria) leucospilota, and Holothuria (Metriatyla) scabra were the 5 timun laut species that grouped under the family Holothuriidae. The outcomes of this study can be utilised by the enforcement agencies to monitor and overcome the issues of species substitution and product mislabelling of processed sea cucumber products in Malaysian markets.
first_indexed 2025-11-14T17:23:07Z
format Article
id iium-70637
institution International Islamic University Malaysia
institution_category Local University
language English
last_indexed 2025-11-14T17:23:07Z
publishDate 2019
publisher Faculty of International Maritime Studies, Kasetsart University
recordtype eprints
repository_type Digital Repository
spelling iium-706372019-07-12T08:03:17Z http://irep.iium.edu.my/70637/ Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes Kamarudin, Kamarul Rahim Mohamed Rehan, Maryam Mohamed Rehan, Aisyah Q Science (General) Species identification of sea cucumbers that have undergone body deformation due to extensive food processing e.g. beche-de-mer is difficult especially with the copresence of cases of unlabelled or mislabelled sea cucumber-based products in the markets. Therefore, a study was done to determine the species identities of processed sea cucumbers from selected Malaysian markets using concatenated gene sequences of non-protein-coding 12S and 16S mitochondrial rRNA genes. Phylogenetic analyses based on the distance-based Neighbour Joining method, and the character-based methods i.e. the Maximum Parsimony method, Maximum Likelihood method, and the Bayesian Analysis method of 47 ingroup sequences representing 37 processed sea cucumber specimens, 6 reference samples, and 4 additional specimens suggested the presence of 3 main clusters i.e. gamat family consisting of genus Stichopus and genus Thelenota; and timun laut family comprising family Holothuriidae. A number of 3 gamat species i.e. Stichopus horrens, Stichopus vastus, and Thelenota anax were recorded. Meanwhile, the specimens of Holothuria (Halodeima) atra, Holothuria (Halodeima) edulis, Holothuria (Metriatyla) lessoni, Holothuria (Mertensiothuria) leucospilota, and Holothuria (Metriatyla) scabra were the 5 timun laut species that grouped under the family Holothuriidae. The outcomes of this study can be utilised by the enforcement agencies to monitor and overcome the issues of species substitution and product mislabelling of processed sea cucumber products in Malaysian markets. Faculty of International Maritime Studies, Kasetsart University 2019 Article PeerReviewed application/pdf en http://irep.iium.edu.my/70637/1/Maritime%20Technology%20and%20Research%202019.pdf Kamarudin, Kamarul Rahim and Mohamed Rehan, Maryam and Mohamed Rehan, Aisyah (2019) Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes. Maritime Technology and Research, 1 (2). pp. 1-19. E-ISSN 2651-205X https://tci-thaijo.org/index.php/MTR/article/view/146259/121339 10.33175/mtr.2019.146259
spellingShingle Q Science (General)
Kamarudin, Kamarul Rahim
Mohamed Rehan, Maryam
Mohamed Rehan, Aisyah
Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes
title Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes
title_full Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes
title_fullStr Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes
title_full_unstemmed Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes
title_short Species identification of processed sea cucumbers from Malaysian market based on concatenated gene sequences of mitochondrial rRNA genes
title_sort species identification of processed sea cucumbers from malaysian market based on concatenated gene sequences of mitochondrial rrna genes
topic Q Science (General)
url http://irep.iium.edu.my/70637/
http://irep.iium.edu.my/70637/
http://irep.iium.edu.my/70637/
http://irep.iium.edu.my/70637/1/Maritime%20Technology%20and%20Research%202019.pdf