Evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct
This study aims to evaluate the cartilaginous extracellular matrix (ECM) production in in vitro poly(lacticco-glycolic acid) PLGA-based scaffolds seeded with rabbit chondrocytes. Scaffolds with the size of 7mm (diameter) × 3mm (height) using ratio of 65:35 were formed using solvent-casting and salt...
| Main Authors: | , , , |
|---|---|
| Format: | Proceeding Paper |
| Language: | English English English |
| Published: |
Institute of Electrical and Electronics Engineers Inc.
2018
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/70459/ http://irep.iium.edu.my/70459/1/70549_Evaluation%20of%20cartilaginous%20extracellular%20matrix.pdf http://irep.iium.edu.my/70459/2/70549_Evaluation%20of%20cartilaginous%20extracellular%20matrix_SCOPUS.pdf http://irep.iium.edu.my/70459/3/70549_Evaluation%20of%20cartilaginous%20extracellular%20matrix_WOS.pdf |
| _version_ | 1848787293198876672 |
|---|---|
| author | Md Ali @ Tahir, Aisyah Hanani Mohamed Amin, Muhammad Azri Ifwat Azhim, Azran Sha'ban, Munirah |
| author_facet | Md Ali @ Tahir, Aisyah Hanani Mohamed Amin, Muhammad Azri Ifwat Azhim, Azran Sha'ban, Munirah |
| author_sort | Md Ali @ Tahir, Aisyah Hanani |
| building | IIUM Repository |
| collection | Online Access |
| description | This study aims to evaluate the cartilaginous extracellular matrix (ECM) production in in vitro poly(lacticco-glycolic acid) PLGA-based scaffolds seeded with rabbit chondrocytes. Scaffolds with the size of 7mm (diameter) × 3mm (height) using ratio of 65:35 were formed using solvent-casting and salt leaching method. The scaffolds were divided into four respective groups, 1) PLGA-atelocollagen-fibrin (PAF), 2) PLGA-atelocollagen (PA), 3) PLGA-fibrin (PF), and 4) PLGA (control). Gross morphology of the scaffold was observed for 3 weeks. Microscopy evaluation and histological staining was done using Haematoxylin and Eosin (H&E), Safranin O, Alcian Blue, Toluidine Blue for week 1, 2 and 3, respectively. The production of sulphated glycosaminoglycan (sGAG) was evaluated using Alcian Blue assay. The PAF group presented better cartilaginous appearance compared to other groups. The hybrid scaffold groups showed more ECM secretion indicated by the presence of web-like fibers network substance on the surface as visualized using scanning electron microscope (SEM). The presence of proteoglycan-rich matrix and glycosaminoglycan has been detected in both histological staining and sGAG assay. Based on the findings of this study, PLGA-based hybrid scaffold promoted better cartilaginous tissue formation in vitro. |
| first_indexed | 2025-11-14T17:22:37Z |
| format | Proceeding Paper |
| id | iium-70459 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:22:37Z |
| publishDate | 2018 |
| publisher | Institute of Electrical and Electronics Engineers Inc. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-704592019-06-13T08:24:25Z http://irep.iium.edu.my/70459/ Evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct Md Ali @ Tahir, Aisyah Hanani Mohamed Amin, Muhammad Azri Ifwat Azhim, Azran Sha'ban, Munirah TA164 Bioengineering This study aims to evaluate the cartilaginous extracellular matrix (ECM) production in in vitro poly(lacticco-glycolic acid) PLGA-based scaffolds seeded with rabbit chondrocytes. Scaffolds with the size of 7mm (diameter) × 3mm (height) using ratio of 65:35 were formed using solvent-casting and salt leaching method. The scaffolds were divided into four respective groups, 1) PLGA-atelocollagen-fibrin (PAF), 2) PLGA-atelocollagen (PA), 3) PLGA-fibrin (PF), and 4) PLGA (control). Gross morphology of the scaffold was observed for 3 weeks. Microscopy evaluation and histological staining was done using Haematoxylin and Eosin (H&E), Safranin O, Alcian Blue, Toluidine Blue for week 1, 2 and 3, respectively. The production of sulphated glycosaminoglycan (sGAG) was evaluated using Alcian Blue assay. The PAF group presented better cartilaginous appearance compared to other groups. The hybrid scaffold groups showed more ECM secretion indicated by the presence of web-like fibers network substance on the surface as visualized using scanning electron microscope (SEM). The presence of proteoglycan-rich matrix and glycosaminoglycan has been detected in both histological staining and sGAG assay. Based on the findings of this study, PLGA-based hybrid scaffold promoted better cartilaginous tissue formation in vitro. Institute of Electrical and Electronics Engineers Inc. 2018-12 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/70459/1/70549_Evaluation%20of%20cartilaginous%20extracellular%20matrix.pdf application/pdf en http://irep.iium.edu.my/70459/2/70549_Evaluation%20of%20cartilaginous%20extracellular%20matrix_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/70459/3/70549_Evaluation%20of%20cartilaginous%20extracellular%20matrix_WOS.pdf Md Ali @ Tahir, Aisyah Hanani and Mohamed Amin, Muhammad Azri Ifwat and Azhim, Azran and Sha'ban, Munirah (2018) Evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct. In: 2018 IEEE EMBS Conference on Biomedical Engineering and Sciences )IECBES 2018), 3rd-6th December 2018, Kuching, Sarawak. https://ieeexplore.ieee.org/document/8626712/authors#authors 10.1109/IECBES.2018.8626712 |
| spellingShingle | TA164 Bioengineering Md Ali @ Tahir, Aisyah Hanani Mohamed Amin, Muhammad Azri Ifwat Azhim, Azran Sha'ban, Munirah Evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct |
| title | Evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct |
| title_full | Evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct |
| title_fullStr | Evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct |
| title_full_unstemmed | Evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct |
| title_short | Evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct |
| title_sort | evaluation of cartilaginous extracellular matrix production in in vitro "cell-scaffold" construct |
| topic | TA164 Bioengineering |
| url | http://irep.iium.edu.my/70459/ http://irep.iium.edu.my/70459/ http://irep.iium.edu.my/70459/ http://irep.iium.edu.my/70459/1/70549_Evaluation%20of%20cartilaginous%20extracellular%20matrix.pdf http://irep.iium.edu.my/70459/2/70549_Evaluation%20of%20cartilaginous%20extracellular%20matrix_SCOPUS.pdf http://irep.iium.edu.my/70459/3/70549_Evaluation%20of%20cartilaginous%20extracellular%20matrix_WOS.pdf |