Construction of metagenomic DNA libraries and enrichment strategies
Microorganisms are the main source of biocatalysts and with metagenomics approach it is now possible to overcome the major obstacle of microbiology which lies on the inability of the vast majority of microbes to grow under laboratory conditions. This chapter introduces and guides readers to a molecu...
| Main Authors: | , , |
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| Format: | Book Chapter |
| Language: | English English |
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Springer Nature
2018
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| Online Access: | http://irep.iium.edu.my/70247/ http://irep.iium.edu.my/70247/1/70247_Construction%20of%20metagenomic%20DNA%20libraries.pdf http://irep.iium.edu.my/70247/7/70247_Construction%20of%20metagenomic%20DNA%20libraries_SCOPUS.pdf |
| _version_ | 1848787256770297856 |
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| author | Benbelgacem, Farah Fadwa Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali |
| author_facet | Benbelgacem, Farah Fadwa Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali |
| author_sort | Benbelgacem, Farah Fadwa |
| building | IIUM Repository |
| collection | Online Access |
| description | Microorganisms are the main source of biocatalysts and with metagenomics approach it is now possible to overcome the major obstacle of microbiology which lies on the inability of the vast majority of microbes to grow under laboratory conditions. This chapter introduces and guides readers to a molecular biology approach that is useful for bioprospecting of novel, efficient and specific enzymes without escaping any unculturable or difficult to culture microorgaims. The first and necessary step of functional metagenomics is the metagenomic DNA library construction. It consists of metagenome extraction from the desired habitat, shearing of the DNA into sequences of desired size, endrepairing and cloning of these DNA sequences into a large-insert and high-copy number vector such as pCC1FOS with EPI300T1R E. coli as surrogate host, to the final step of storing the clones in glycerol stock for long term storage. Enrichment pre-DNA extraction step may be adopted to increase the likelihood that metagenomic DNA libraries hold the genes for the desired enzymatic activities. |
| first_indexed | 2025-11-14T17:22:03Z |
| format | Book Chapter |
| id | iium-70247 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:22:03Z |
| publishDate | 2018 |
| publisher | Springer Nature |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-702472021-06-18T02:33:40Z http://irep.iium.edu.my/70247/ Construction of metagenomic DNA libraries and enrichment strategies Benbelgacem, Farah Fadwa Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali TP200 Manufacture and use of chemicals TP248.13 Biotechnology Microorganisms are the main source of biocatalysts and with metagenomics approach it is now possible to overcome the major obstacle of microbiology which lies on the inability of the vast majority of microbes to grow under laboratory conditions. This chapter introduces and guides readers to a molecular biology approach that is useful for bioprospecting of novel, efficient and specific enzymes without escaping any unculturable or difficult to culture microorgaims. The first and necessary step of functional metagenomics is the metagenomic DNA library construction. It consists of metagenome extraction from the desired habitat, shearing of the DNA into sequences of desired size, endrepairing and cloning of these DNA sequences into a large-insert and high-copy number vector such as pCC1FOS with EPI300T1R E. coli as surrogate host, to the final step of storing the clones in glycerol stock for long term storage. Enrichment pre-DNA extraction step may be adopted to increase the likelihood that metagenomic DNA libraries hold the genes for the desired enzymatic activities. Springer Nature 2018 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/70247/1/70247_Construction%20of%20metagenomic%20DNA%20libraries.pdf application/pdf en http://irep.iium.edu.my/70247/7/70247_Construction%20of%20metagenomic%20DNA%20libraries_SCOPUS.pdf Benbelgacem, Farah Fadwa and Mohd. Salleh, Hamzah and Noorbatcha, Ibrahim Ali (2018) Construction of metagenomic DNA libraries and enrichment strategies. In: Multifaceted protocol in biotechnology. Springer Nature, Singapore, pp. 23-42. ISBN 978-981-13-2256-3 https://www.springer.com/us/book/9789811322563 |
| spellingShingle | TP200 Manufacture and use of chemicals TP248.13 Biotechnology Benbelgacem, Farah Fadwa Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali Construction of metagenomic DNA libraries and enrichment strategies |
| title | Construction of metagenomic DNA libraries and enrichment strategies |
| title_full | Construction of metagenomic DNA libraries and enrichment strategies |
| title_fullStr | Construction of metagenomic DNA libraries and enrichment strategies |
| title_full_unstemmed | Construction of metagenomic DNA libraries and enrichment strategies |
| title_short | Construction of metagenomic DNA libraries and enrichment strategies |
| title_sort | construction of metagenomic dna libraries and enrichment strategies |
| topic | TP200 Manufacture and use of chemicals TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/70247/ http://irep.iium.edu.my/70247/ http://irep.iium.edu.my/70247/1/70247_Construction%20of%20metagenomic%20DNA%20libraries.pdf http://irep.iium.edu.my/70247/7/70247_Construction%20of%20metagenomic%20DNA%20libraries_SCOPUS.pdf |