Site-directed mutagenesis on plasmid using polymerase chain reaction
Site-directed mutagenesis (SDM) is a very useful technique to study changes in protein function that may occur as a result of the DNA manipulation. A detail procedure to employ SDM on whole plasmid using polymerase chain reaction (PCR) with successful application of the method on a pET28a plasmid ha...
| Main Authors: | , , |
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| Format: | Book Chapter |
| Language: | English English |
| Published: |
Springer Nature
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/70243/ http://irep.iium.edu.my/70243/1/70243_Site-directed%20mutagenesis%20on%20plasmid.pdf http://irep.iium.edu.my/70243/7/70243_Site-directed%20mutagenesis%20on%20plasmid_Scopus.pdf |
| _version_ | 1848787255909416960 |
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| author | Ahmad, Abdul Aziz Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali |
| author_facet | Ahmad, Abdul Aziz Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali |
| author_sort | Ahmad, Abdul Aziz |
| building | IIUM Repository |
| collection | Online Access |
| description | Site-directed mutagenesis (SDM) is a very useful technique to study changes in protein function that may occur as a result of the DNA manipulation. A detail procedure to employ SDM on whole plasmid using polymerase chain reaction (PCR) with successful application of the method on a pET28a plasmid harboring an endoglucanase I gene from Fusarium oxysporum is described here. |
| first_indexed | 2025-11-14T17:22:02Z |
| format | Book Chapter |
| id | iium-70243 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:22:02Z |
| publishDate | 2018 |
| publisher | Springer Nature |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-702432021-06-18T02:30:07Z http://irep.iium.edu.my/70243/ Site-directed mutagenesis on plasmid using polymerase chain reaction Ahmad, Abdul Aziz Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali TP200 Manufacture and use of chemicals TP248.13 Biotechnology Site-directed mutagenesis (SDM) is a very useful technique to study changes in protein function that may occur as a result of the DNA manipulation. A detail procedure to employ SDM on whole plasmid using polymerase chain reaction (PCR) with successful application of the method on a pET28a plasmid harboring an endoglucanase I gene from Fusarium oxysporum is described here. Springer Nature 2018 Book Chapter PeerReviewed application/pdf en http://irep.iium.edu.my/70243/1/70243_Site-directed%20mutagenesis%20on%20plasmid.pdf application/pdf en http://irep.iium.edu.my/70243/7/70243_Site-directed%20mutagenesis%20on%20plasmid_Scopus.pdf Ahmad, Abdul Aziz and Mohd. Salleh, Hamzah and Noorbatcha, Ibrahim Ali (2018) Site-directed mutagenesis on plasmid using polymerase chain reaction. In: Multifaceted protocol in biotechnology. Springer Nature, Singapore, pp. 1-12. ISBN 978-981-13-2256-3 https://www.springer.com/us/book/9789811322563 https://doi.org/10.1007/978-981-13-2257-0 |
| spellingShingle | TP200 Manufacture and use of chemicals TP248.13 Biotechnology Ahmad, Abdul Aziz Mohd. Salleh, Hamzah Noorbatcha, Ibrahim Ali Site-directed mutagenesis on plasmid using polymerase chain reaction |
| title | Site-directed mutagenesis on plasmid using polymerase chain reaction |
| title_full | Site-directed mutagenesis on plasmid using polymerase chain reaction |
| title_fullStr | Site-directed mutagenesis on plasmid using polymerase chain reaction |
| title_full_unstemmed | Site-directed mutagenesis on plasmid using polymerase chain reaction |
| title_short | Site-directed mutagenesis on plasmid using polymerase chain reaction |
| title_sort | site-directed mutagenesis on plasmid using polymerase chain reaction |
| topic | TP200 Manufacture and use of chemicals TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/70243/ http://irep.iium.edu.my/70243/ http://irep.iium.edu.my/70243/ http://irep.iium.edu.my/70243/1/70243_Site-directed%20mutagenesis%20on%20plasmid.pdf http://irep.iium.edu.my/70243/7/70243_Site-directed%20mutagenesis%20on%20plasmid_Scopus.pdf |