Phylogenetic study of presumptive oil-degrading microbes isolated from the North-Western tip of Pahang
Many construction areas are often contaminated with petroleum compounds. The aim of this work were to isolate and characterize indigenous bacteria isolated at a moderate temperature site as well as to study the pattern of phylogenetic tree among bacterial communities associated with oil degradation....
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| Format: | Article |
| Language: | English English English |
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Penerbit UTHM
2018
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| Online Access: | http://irep.iium.edu.my/69676/ http://irep.iium.edu.my/69676/1/69676_Phylogenetic%20Study%20of%20Presumptive%20Oil-degrading.pdf http://irep.iium.edu.my/69676/2/69676_Phylogenetic%20Study%20of%20Presumptive%20Oil-degrading_SCOPUS.pdf http://irep.iium.edu.my/69676/3/69676_Phylogenetic%20Study%20of%20Presumptive%20Oil-degrading_WOS.pdf |
| _version_ | 1848787156657504256 |
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| author | Azizan, Nur Hafizah Md Rami, Siti Zakiah Saedudin, RD Rohmat Kasim, Shahreen |
| author_facet | Azizan, Nur Hafizah Md Rami, Siti Zakiah Saedudin, RD Rohmat Kasim, Shahreen |
| author_sort | Azizan, Nur Hafizah |
| building | IIUM Repository |
| collection | Online Access |
| description | Many construction areas are often contaminated with petroleum compounds. The aim of this work were to isolate and characterize indigenous bacteria isolated at a moderate temperature site as well as to study the pattern of phylogenetic tree among bacterial communities associated with oil degradation. No profound studies have yet been done in the construction site at Tanah Rata. Hence, this research was carried out to find existing status of microbial community from a few selected spots. Enrichment culture technique by using MSM broth has been used to isolate the desired microorganisms. Isolation and characterization tests using phenotypic and genotypic approaches (based on genes encoding 16S rRNA) had led to the discovery of 18 isolates. The 16S rRNA was used due to its functional constant, universally distributed and moderately well discovered across broad phylogenetic distances. The successfully identified genera were Pseudomonas, Bacillus, Exiguobacterium, Stenotrophomonas, Acinetobacter, Serratia and Gamma Proteobacterium. |
| first_indexed | 2025-11-14T17:20:27Z |
| format | Article |
| id | iium-69676 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:20:27Z |
| publishDate | 2018 |
| publisher | Penerbit UTHM |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-696762019-01-29T04:15:08Z http://irep.iium.edu.my/69676/ Phylogenetic study of presumptive oil-degrading microbes isolated from the North-Western tip of Pahang Azizan, Nur Hafizah Md Rami, Siti Zakiah Saedudin, RD Rohmat Kasim, Shahreen Q Science (General) Many construction areas are often contaminated with petroleum compounds. The aim of this work were to isolate and characterize indigenous bacteria isolated at a moderate temperature site as well as to study the pattern of phylogenetic tree among bacterial communities associated with oil degradation. No profound studies have yet been done in the construction site at Tanah Rata. Hence, this research was carried out to find existing status of microbial community from a few selected spots. Enrichment culture technique by using MSM broth has been used to isolate the desired microorganisms. Isolation and characterization tests using phenotypic and genotypic approaches (based on genes encoding 16S rRNA) had led to the discovery of 18 isolates. The 16S rRNA was used due to its functional constant, universally distributed and moderately well discovered across broad phylogenetic distances. The successfully identified genera were Pseudomonas, Bacillus, Exiguobacterium, Stenotrophomonas, Acinetobacter, Serratia and Gamma Proteobacterium. Penerbit UTHM 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/69676/1/69676_Phylogenetic%20Study%20of%20Presumptive%20Oil-degrading.pdf application/pdf en http://irep.iium.edu.my/69676/2/69676_Phylogenetic%20Study%20of%20Presumptive%20Oil-degrading_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/69676/3/69676_Phylogenetic%20Study%20of%20Presumptive%20Oil-degrading_WOS.pdf Azizan, Nur Hafizah and Md Rami, Siti Zakiah and Saedudin, RD Rohmat and Kasim, Shahreen (2018) Phylogenetic study of presumptive oil-degrading microbes isolated from the North-Western tip of Pahang. International Journal of Integrated Engineering, 10 (6). pp. 128-132. ISSN 2229-838X E-ISSN 2600-7916 http://penerbit.uthm.edu.my/ojs/index.php/ijie/article/view/2773/1771 https://doi.org/10.30880/ijie.2018.10.06.017 |
| spellingShingle | Q Science (General) Azizan, Nur Hafizah Md Rami, Siti Zakiah Saedudin, RD Rohmat Kasim, Shahreen Phylogenetic study of presumptive oil-degrading microbes isolated from the North-Western tip of Pahang |
| title | Phylogenetic study of presumptive oil-degrading microbes isolated from the North-Western tip of Pahang |
| title_full | Phylogenetic study of presumptive oil-degrading microbes isolated from the North-Western tip of Pahang |
| title_fullStr | Phylogenetic study of presumptive oil-degrading microbes isolated from the North-Western tip of Pahang |
| title_full_unstemmed | Phylogenetic study of presumptive oil-degrading microbes isolated from the North-Western tip of Pahang |
| title_short | Phylogenetic study of presumptive oil-degrading microbes isolated from the North-Western tip of Pahang |
| title_sort | phylogenetic study of presumptive oil-degrading microbes isolated from the north-western tip of pahang |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/69676/ http://irep.iium.edu.my/69676/ http://irep.iium.edu.my/69676/ http://irep.iium.edu.my/69676/1/69676_Phylogenetic%20Study%20of%20Presumptive%20Oil-degrading.pdf http://irep.iium.edu.my/69676/2/69676_Phylogenetic%20Study%20of%20Presumptive%20Oil-degrading_SCOPUS.pdf http://irep.iium.edu.my/69676/3/69676_Phylogenetic%20Study%20of%20Presumptive%20Oil-degrading_WOS.pdf |