ATR-FTIR and spectroscopic methods for analysis of black seed oil from alginate beads
Objective: This study aimed to use attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR) for rapid quantification of black seed oil (BSO) from alginate beads and to develop simple analysis method for in vitro release study based on turbidity measurement. Methods: Guluronic...
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2018
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| Online Access: | http://irep.iium.edu.my/68932/ http://irep.iium.edu.my/68932/1/ATR-FTIR%20AND%20SPECTROSCOPIC%20METHODS%20FOR%20ANALYSIS%20OF%20BLACK%20SEED%20OIL.pdf http://irep.iium.edu.my/68932/7/Scopus%20-%20ATR-FTIR.pdf |
| _version_ | 1848787027092307968 |
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| author | Alkhatib, Hamzeh Mohamed, Farahidah Doolaanea, Abd Almonem |
| author_facet | Alkhatib, Hamzeh Mohamed, Farahidah Doolaanea, Abd Almonem |
| author_sort | Alkhatib, Hamzeh |
| building | IIUM Repository |
| collection | Online Access |
| description | Objective: This study aimed to use attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR) for rapid quantification of black seed oil (BSO) from alginate beads and to develop simple analysis method for in vitro release study based on turbidity measurement. Methods: Guluronic acid-rich (high-G) sodium alginate was used to encapsulate BSO with the aid of Tween 80 as a stabilizer. ATR-FTIR method was developed for quantification of encapsulation efficiency of BSO by applying Beer-Lambert law after selection of a few wave number combinations. UV-vis method based on measurement of emulsion turbidity at 600 nm was also developed to quantify BSO during the releasing from alginate beads in simulated intestine buffer. Results: ATR-FTIR method exhibited linearity in the range of 25-300 mg BSO /ml emulsion (mg BSO /ml E )with R 2 =0.998, RSD=8.4%, LOD=0.28 mg BSO /ml E and LOQ=0.87 mg BSO /ml E . BSO-alginate beads was found to completely encapsulate BSO with around 100.5% efficiency. UV-vis method exhibited linearity in the range of 50-300 mg BSO /ml E , R 2 =0.9931, RSD=1.34%, LOD=0.89 mg BSO /ml E, and LOQ=2.71 mg BSO /ml E . In addition, the method showed that total amount of BSO was released at 110 min. Conclusion: These methods are considered as a practical method for quantification of BSO for encapsulation efficacy and release. They will help to accelerate and improve routine characterization of encapsulated BSO in food and pharmaceutical technology. |
| first_indexed | 2025-11-14T17:18:24Z |
| format | Article |
| id | iium-68932 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:18:24Z |
| publishDate | 2018 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-689322018-12-26T07:34:25Z http://irep.iium.edu.my/68932/ ATR-FTIR and spectroscopic methods for analysis of black seed oil from alginate beads Alkhatib, Hamzeh Mohamed, Farahidah Doolaanea, Abd Almonem RS192 Materia Medica-Pharmaceutical Technology Objective: This study aimed to use attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR) for rapid quantification of black seed oil (BSO) from alginate beads and to develop simple analysis method for in vitro release study based on turbidity measurement. Methods: Guluronic acid-rich (high-G) sodium alginate was used to encapsulate BSO with the aid of Tween 80 as a stabilizer. ATR-FTIR method was developed for quantification of encapsulation efficiency of BSO by applying Beer-Lambert law after selection of a few wave number combinations. UV-vis method based on measurement of emulsion turbidity at 600 nm was also developed to quantify BSO during the releasing from alginate beads in simulated intestine buffer. Results: ATR-FTIR method exhibited linearity in the range of 25-300 mg BSO /ml emulsion (mg BSO /ml E )with R 2 =0.998, RSD=8.4%, LOD=0.28 mg BSO /ml E and LOQ=0.87 mg BSO /ml E . BSO-alginate beads was found to completely encapsulate BSO with around 100.5% efficiency. UV-vis method exhibited linearity in the range of 50-300 mg BSO /ml E , R 2 =0.9931, RSD=1.34%, LOD=0.89 mg BSO /ml E, and LOQ=2.71 mg BSO /ml E . In addition, the method showed that total amount of BSO was released at 110 min. Conclusion: These methods are considered as a practical method for quantification of BSO for encapsulation efficacy and release. They will help to accelerate and improve routine characterization of encapsulated BSO in food and pharmaceutical technology. 2018-07 Article PeerReviewed application/pdf en http://irep.iium.edu.my/68932/1/ATR-FTIR%20AND%20SPECTROSCOPIC%20METHODS%20FOR%20ANALYSIS%20OF%20BLACK%20SEED%20OIL.pdf application/pdf en http://irep.iium.edu.my/68932/7/Scopus%20-%20ATR-FTIR.pdf Alkhatib, Hamzeh and Mohamed, Farahidah and Doolaanea, Abd Almonem (2018) ATR-FTIR and spectroscopic methods for analysis of black seed oil from alginate beads. International Journal of Applied Pharmaceutics, 10 (5). pp. 147-152. ISSN 0975-7058 https://innovareacademics.in/journals/index.php/ijap/article/view/25057/15688 10.22159/ijap.2018v10i5.25057 |
| spellingShingle | RS192 Materia Medica-Pharmaceutical Technology Alkhatib, Hamzeh Mohamed, Farahidah Doolaanea, Abd Almonem ATR-FTIR and spectroscopic methods for analysis of black seed oil from alginate beads |
| title | ATR-FTIR and spectroscopic methods for analysis of black seed oil from alginate beads |
| title_full | ATR-FTIR and spectroscopic methods for analysis of black seed oil from alginate beads |
| title_fullStr | ATR-FTIR and spectroscopic methods for analysis of black seed oil from alginate beads |
| title_full_unstemmed | ATR-FTIR and spectroscopic methods for analysis of black seed oil from alginate beads |
| title_short | ATR-FTIR and spectroscopic methods for analysis of black seed oil from alginate beads |
| title_sort | atr-ftir and spectroscopic methods for analysis of black seed oil from alginate beads |
| topic | RS192 Materia Medica-Pharmaceutical Technology |
| url | http://irep.iium.edu.my/68932/ http://irep.iium.edu.my/68932/ http://irep.iium.edu.my/68932/ http://irep.iium.edu.my/68932/1/ATR-FTIR%20AND%20SPECTROSCOPIC%20METHODS%20FOR%20ANALYSIS%20OF%20BLACK%20SEED%20OIL.pdf http://irep.iium.edu.my/68932/7/Scopus%20-%20ATR-FTIR.pdf |