Quantifying E. coli O157:H7 via linear sweep voltammetry in phosphate buffered saline and tap water
E. coli O157:H7 is a common microorganism that is responsible for contaminating water after major floods events. Standard methods of pathogen detection cannot be utilized in the field; the need for a portable immunosensor becomes urgent so detection of pathogens can be done at remote post-flood ar...
| Main Authors: | , , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
Kulliyyah of Engineering International Islamic University Malaysia
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/68448/ http://irep.iium.edu.my/68448/1/PROCEEDING%20BOOK%20ICBioE%202018.pdf |
| _version_ | 1848786944722468864 |
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| author | Ali Ahmed, Nasteho Ramli, Nurul Izzati Abdul Jalal, Nurfatin Azma Abd. Wahab, Mohd. Firdaus Wan Salim, Wan Wardatul Amani |
| author_facet | Ali Ahmed, Nasteho Ramli, Nurul Izzati Abdul Jalal, Nurfatin Azma Abd. Wahab, Mohd. Firdaus Wan Salim, Wan Wardatul Amani |
| author_sort | Ali Ahmed, Nasteho |
| building | IIUM Repository |
| collection | Online Access |
| description | E. coli O157:H7 is a common microorganism that is responsible for contaminating water after major floods
events. Standard methods of pathogen detection cannot be utilized in the field; the need for a portable
immunosensor becomes urgent so detection of pathogens can be done at remote post-flood areas with no lab
and technician access. In this study, the objectives are to analyze E. coli binding to reduced graphene oxide
(rGO) electrode surfaces via Linear Sweep Voltammetry (LSV) in phosphate buffered saline (PBS), pH 7.4,
and potable tap water, and to correlate voltammetry results with E. coli attachment on electrodes by using
FESEM. From the results, LSV in PBS shows sensitivity of 38.7 µA/(cell/ml) in comparison to tap water, with
a lower sensitivity of 2.20 µA/(cell/ml). Linear range from 103 to 108 cells/µl was achieved in both solution types.
FESEM results show higher attachment of cells on rGO-based electrodes for higher concentration of cells,
confirming the lowering of current in LSV measurements owing to increased cell attachment. |
| first_indexed | 2025-11-14T17:17:05Z |
| format | Proceeding Paper |
| id | iium-68448 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T17:17:05Z |
| publishDate | 2018 |
| publisher | Kulliyyah of Engineering International Islamic University Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-684482019-07-03T07:29:24Z http://irep.iium.edu.my/68448/ Quantifying E. coli O157:H7 via linear sweep voltammetry in phosphate buffered saline and tap water Ali Ahmed, Nasteho Ramli, Nurul Izzati Abdul Jalal, Nurfatin Azma Abd. Wahab, Mohd. Firdaus Wan Salim, Wan Wardatul Amani QD Chemistry TD169 Environmental protection TP155 Chemical engineering TP248.13 Biotechnology E. coli O157:H7 is a common microorganism that is responsible for contaminating water after major floods events. Standard methods of pathogen detection cannot be utilized in the field; the need for a portable immunosensor becomes urgent so detection of pathogens can be done at remote post-flood areas with no lab and technician access. In this study, the objectives are to analyze E. coli binding to reduced graphene oxide (rGO) electrode surfaces via Linear Sweep Voltammetry (LSV) in phosphate buffered saline (PBS), pH 7.4, and potable tap water, and to correlate voltammetry results with E. coli attachment on electrodes by using FESEM. From the results, LSV in PBS shows sensitivity of 38.7 µA/(cell/ml) in comparison to tap water, with a lower sensitivity of 2.20 µA/(cell/ml). Linear range from 103 to 108 cells/µl was achieved in both solution types. FESEM results show higher attachment of cells on rGO-based electrodes for higher concentration of cells, confirming the lowering of current in LSV measurements owing to increased cell attachment. Kulliyyah of Engineering International Islamic University Malaysia 2018-09-19 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/68448/1/PROCEEDING%20BOOK%20ICBioE%202018.pdf Ali Ahmed, Nasteho and Ramli, Nurul Izzati and Abdul Jalal, Nurfatin Azma and Abd. Wahab, Mohd. Firdaus and Wan Salim, Wan Wardatul Amani (2018) Quantifying E. coli O157:H7 via linear sweep voltammetry in phosphate buffered saline and tap water. In: Proceedings of the International Conference Biotechnology Engineering, ICBioE ‘18, 19-20 September 2018, Gombak, Kuala Lumpur. |
| spellingShingle | QD Chemistry TD169 Environmental protection TP155 Chemical engineering TP248.13 Biotechnology Ali Ahmed, Nasteho Ramli, Nurul Izzati Abdul Jalal, Nurfatin Azma Abd. Wahab, Mohd. Firdaus Wan Salim, Wan Wardatul Amani Quantifying E. coli O157:H7 via linear sweep voltammetry in phosphate buffered saline and tap water |
| title | Quantifying E. coli O157:H7 via linear sweep voltammetry in
phosphate buffered saline and tap water |
| title_full | Quantifying E. coli O157:H7 via linear sweep voltammetry in
phosphate buffered saline and tap water |
| title_fullStr | Quantifying E. coli O157:H7 via linear sweep voltammetry in
phosphate buffered saline and tap water |
| title_full_unstemmed | Quantifying E. coli O157:H7 via linear sweep voltammetry in
phosphate buffered saline and tap water |
| title_short | Quantifying E. coli O157:H7 via linear sweep voltammetry in
phosphate buffered saline and tap water |
| title_sort | quantifying e. coli o157:h7 via linear sweep voltammetry in
phosphate buffered saline and tap water |
| topic | QD Chemistry TD169 Environmental protection TP155 Chemical engineering TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/68448/ http://irep.iium.edu.my/68448/1/PROCEEDING%20BOOK%20ICBioE%202018.pdf |