Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry
Existing practice of glucose-level monitoring for diabetic patients involves frequent finger pricking for blood samples. This is not only constantly painful, but could make patients susceptible to infections. A non-invasive, portable, graphene-based biosensor is being developed to measure glucose l...
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| Format: | Proceeding Paper |
| Language: | English English |
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AIP
2018
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| Online Access: | http://irep.iium.edu.my/68443/ http://irep.iium.edu.my/68443/1/ISTEC%202018%20AIP%20Conference%20Proceeding%20Nurul%20Izzati%20et%20al%202018.pdf http://irep.iium.edu.my/68443/7/68443%20Characterization%20of%20Enzymatic%20Glucose%20Biosensor%20in%20Buffer.pdf |
| _version_ | 1848786943164284928 |
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| author | Ramli, Nurul Izzati Abd-Wahab, Firdaus Wan Salim, Wan Wardatul Amani |
| author_facet | Ramli, Nurul Izzati Abd-Wahab, Firdaus Wan Salim, Wan Wardatul Amani |
| author_sort | Ramli, Nurul Izzati |
| building | IIUM Repository |
| collection | Online Access |
| description | Existing practice of glucose-level monitoring for diabetic patients involves frequent finger pricking for blood
samples. This is not only constantly painful, but could make patients susceptible to infections. A non-invasive, portable, graphene-based biosensor is being developed to measure glucose levels in saliva instead of in blood, by incorporating rGO-PEDOT:PSS composites as the transducer layer on a working electrode – a screen-printed carbon electrode (SPCE) 2 mm in diameter. To evaluate the performance of the biosensor, the electrochemical technique of linear sweep voltammetry (LSV) was used. The results showed sensitivity of rGO-PEDOT:PSS-GOx/SPCE in artificial saliva: 105 μA/mM (σ = 12.52) at no glucose to 0.2 mM glucose concentrations and 35.33 μA/mM (σ = 5.79) at glucose concentrations of 0.2 mM to 0.6 mM, with correlation coefficient of 0.99. The sensitivity of the biosensor in 10 mM PBS (pH 6.8) is 9.88 μA/mM (σ = 2.74), while in 100 mM potassium ferricyanide it is 10.48 μA/mM (σ = 0.49). The high sensitivity obtained in artificial saliva suggests that the rGO-PEDOT:PSS composite is suitable for use as a transducer layer for non-invasive glucose monitoring. |
| first_indexed | 2025-11-14T17:17:04Z |
| format | Proceeding Paper |
| id | iium-68443 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:17:04Z |
| publishDate | 2018 |
| publisher | AIP |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-684432018-12-17T06:18:37Z http://irep.iium.edu.my/68443/ Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry Ramli, Nurul Izzati Abd-Wahab, Firdaus Wan Salim, Wan Wardatul Amani TP155 Chemical engineering TP248.13 Biotechnology Existing practice of glucose-level monitoring for diabetic patients involves frequent finger pricking for blood samples. This is not only constantly painful, but could make patients susceptible to infections. A non-invasive, portable, graphene-based biosensor is being developed to measure glucose levels in saliva instead of in blood, by incorporating rGO-PEDOT:PSS composites as the transducer layer on a working electrode – a screen-printed carbon electrode (SPCE) 2 mm in diameter. To evaluate the performance of the biosensor, the electrochemical technique of linear sweep voltammetry (LSV) was used. The results showed sensitivity of rGO-PEDOT:PSS-GOx/SPCE in artificial saliva: 105 μA/mM (σ = 12.52) at no glucose to 0.2 mM glucose concentrations and 35.33 μA/mM (σ = 5.79) at glucose concentrations of 0.2 mM to 0.6 mM, with correlation coefficient of 0.99. The sensitivity of the biosensor in 10 mM PBS (pH 6.8) is 9.88 μA/mM (σ = 2.74), while in 100 mM potassium ferricyanide it is 10.48 μA/mM (σ = 0.49). The high sensitivity obtained in artificial saliva suggests that the rGO-PEDOT:PSS composite is suitable for use as a transducer layer for non-invasive glucose monitoring. AIP 2018-11-29 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/68443/1/ISTEC%202018%20AIP%20Conference%20Proceeding%20Nurul%20Izzati%20et%20al%202018.pdf application/pdf en http://irep.iium.edu.my/68443/7/68443%20Characterization%20of%20Enzymatic%20Glucose%20Biosensor%20in%20Buffer.pdf Ramli, Nurul Izzati and Abd-Wahab, Firdaus and Wan Salim, Wan Wardatul Amani (2018) Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry. In: 3rd International Sciences, Technology & Engineering Conference (ISTEC) 2018 - Material Chemistry, 18th April 2018, Penang, Malaysia. https://aip.scitation.org/doi/10.1063/1.5066993 10.1063/1.5066993 |
| spellingShingle | TP155 Chemical engineering TP248.13 Biotechnology Ramli, Nurul Izzati Abd-Wahab, Firdaus Wan Salim, Wan Wardatul Amani Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry |
| title | Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry |
| title_full | Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry |
| title_fullStr | Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry |
| title_full_unstemmed | Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry |
| title_short | Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry |
| title_sort | characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry |
| topic | TP155 Chemical engineering TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/68443/ http://irep.iium.edu.my/68443/ http://irep.iium.edu.my/68443/ http://irep.iium.edu.my/68443/1/ISTEC%202018%20AIP%20Conference%20Proceeding%20Nurul%20Izzati%20et%20al%202018.pdf http://irep.iium.edu.my/68443/7/68443%20Characterization%20of%20Enzymatic%20Glucose%20Biosensor%20in%20Buffer.pdf |