Biosynthesis of nanoparticles using recombinant bromelain
Green chemical method was applied to synthesize nanoparticles using recombinant bromelain. Among the numerous applications of recombinant bromelain, there is still no research on nanoparticles synthesis which encourages its utilization in this study. Four chemicals which are copper (II) chloride...
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| Format: | Article |
| Language: | English |
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IIUM Press, International Islamic University Malaysia
2018
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| Online Access: | http://irep.iium.edu.my/68285/ http://irep.iium.edu.my/68285/1/68285_Biosynthesis%20of%20nanoparticles.pdf |
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| author | Nasruddin, Arina Amid, Azura Sulaiman, Sarina Othman, Muhd. Ezza Faiez |
| author_facet | Nasruddin, Arina Amid, Azura Sulaiman, Sarina Othman, Muhd. Ezza Faiez |
| author_sort | Nasruddin, Arina |
| building | IIUM Repository |
| collection | Online Access |
| description | Green chemical method was applied to synthesize nanoparticles using recombinant
bromelain. Among the numerous applications of recombinant bromelain, there is still no research
on nanoparticles synthesis which encourages its utilization in this study. Four chemicals which are
copper (II) chloride dihydrate (CuCl2.2H2O), cerium nitrate hexahydrate (Ce(NO3)3.6H2O), sodium
selenite (Na2SeO3), and iron (III) chloride hexahydrate (FeCl3.6H2O) were selected to be screened
for the suitability in nanoparticles biosynthesis by recombinant bromelain. The nanoparticles
formed were characterized by using UV-visible absorption spectra. The biosynthesis process then
was optimized by varying the centrifugation speed, temperature, and time to get the maximum
absorption and weight of nanoparticles through central composite design (CCD) tool. Only
CuCl2.2H2O showed a positive result for the screening process which was represented by the
formation of colloidal solution and a maximum absorption at 580 nm. Thus, optimization was
carried out for this chemical. Based on the optimization model, maximum absorption and weight
were predicted at 67.5°C, 2 hrs, and 9,600 rpm. These optimal conditions were validated by
repeating the biosynthesis process. The absorption and weight of the nanoparticles depended on the
reaction of the chemical with recombinant bromelain. 3D plots showed that the optimal condition
for high responses mostly depends on temperature and time. |
| first_indexed | 2025-11-14T17:16:35Z |
| format | Article |
| id | iium-68285 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T17:16:35Z |
| publishDate | 2018 |
| publisher | IIUM Press, International Islamic University Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-682852019-07-11T02:11:04Z http://irep.iium.edu.my/68285/ Biosynthesis of nanoparticles using recombinant bromelain Nasruddin, Arina Amid, Azura Sulaiman, Sarina Othman, Muhd. Ezza Faiez TP368 Food processing and manufacture Green chemical method was applied to synthesize nanoparticles using recombinant bromelain. Among the numerous applications of recombinant bromelain, there is still no research on nanoparticles synthesis which encourages its utilization in this study. Four chemicals which are copper (II) chloride dihydrate (CuCl2.2H2O), cerium nitrate hexahydrate (Ce(NO3)3.6H2O), sodium selenite (Na2SeO3), and iron (III) chloride hexahydrate (FeCl3.6H2O) were selected to be screened for the suitability in nanoparticles biosynthesis by recombinant bromelain. The nanoparticles formed were characterized by using UV-visible absorption spectra. The biosynthesis process then was optimized by varying the centrifugation speed, temperature, and time to get the maximum absorption and weight of nanoparticles through central composite design (CCD) tool. Only CuCl2.2H2O showed a positive result for the screening process which was represented by the formation of colloidal solution and a maximum absorption at 580 nm. Thus, optimization was carried out for this chemical. Based on the optimization model, maximum absorption and weight were predicted at 67.5°C, 2 hrs, and 9,600 rpm. These optimal conditions were validated by repeating the biosynthesis process. The absorption and weight of the nanoparticles depended on the reaction of the chemical with recombinant bromelain. 3D plots showed that the optimal condition for high responses mostly depends on temperature and time. IIUM Press, International Islamic University Malaysia 2018-11-29 Article PeerReviewed application/pdf en http://irep.iium.edu.my/68285/1/68285_Biosynthesis%20of%20nanoparticles.pdf Nasruddin, Arina and Amid, Azura and Sulaiman, Sarina and Othman, Muhd. Ezza Faiez (2018) Biosynthesis of nanoparticles using recombinant bromelain. Biological and Natural Resources Engineering Journal, 1 (1). pp. 14-25. E-ISSN 2637-0719 http://journals.iium.edu.my/bnrej/index.php/bnrej/issue/current |
| spellingShingle | TP368 Food processing and manufacture Nasruddin, Arina Amid, Azura Sulaiman, Sarina Othman, Muhd. Ezza Faiez Biosynthesis of nanoparticles using recombinant bromelain |
| title | Biosynthesis of nanoparticles using recombinant bromelain |
| title_full | Biosynthesis of nanoparticles using recombinant bromelain |
| title_fullStr | Biosynthesis of nanoparticles using recombinant bromelain |
| title_full_unstemmed | Biosynthesis of nanoparticles using recombinant bromelain |
| title_short | Biosynthesis of nanoparticles using recombinant bromelain |
| title_sort | biosynthesis of nanoparticles using recombinant bromelain |
| topic | TP368 Food processing and manufacture |
| url | http://irep.iium.edu.my/68285/ http://irep.iium.edu.my/68285/ http://irep.iium.edu.my/68285/1/68285_Biosynthesis%20of%20nanoparticles.pdf |