Cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract
Background: In practice, either the herbs themselves or the crude extracts are used for traditional remedies but not the isolated molecules. Therefore, the importance of toxicity and clot lysis study of the crude extract is beyond description. Objective: The aim of our study was to find out th...
| Main Authors: | , , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/68159/ http://irep.iium.edu.my/68159/1/IPRC-2018-1.pdf |
| _version_ | 1848786891375116288 |
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| author | Azad, A.K. Sarker, Md. Zaidul Islam Helal Uddin, A.B.M. Alaama, Mohamed Abdullah, J. |
| author_facet | Azad, A.K. Sarker, Md. Zaidul Islam Helal Uddin, A.B.M. Alaama, Mohamed Abdullah, J. |
| author_sort | Azad, A.K. |
| building | IIUM Repository |
| collection | Online Access |
| description | Background:
In practice, either the herbs themselves or the crude extracts are used for traditional remedies but not the isolated molecules. Therefore, the importance of toxicity and clot lysis study of the crude extract is beyond description.
Objective:
The aim of our study was to find out the preliminary cytotoxic and thrombolytic effect of the seven selected medicinal plant leaves extract.
Methods:
Cold maceration was performed using ethanol for extraction purposes. The cytotoxicity study was done by using brine shrimp
nauplii (Artemia salina) and MTT assays by using MCF-7 cell
line.
Result:
In the cytotoxic activity, out of the seven leaves extract three of them exhibited stronger brine shrimp lethality with 4 TH PHARMACEUTICAL RESEARCH CONFERENCE 2018
Challenging The Inquisitive Minds: Frontier Of The Future
24 – 26 August 2018 | Cyberjaya, Malaysia
LC50 122.548 (Uncaria acida), 170.861 (Leea indica) and
175.469 (Piper porphyrophyllum) μg /mL respectively, and on
MCF-7 cell line, they also exhibited moderate cytotoxic activity with different concentration of the extract of the same plant leaves such as, Uncaria acida (72.31, 56.22, 38.12 and 9.24%), Leea indica (67.31, 58.22, 43.12 and 15.24%), Piper porphyrophyllum (65.88, 48.12, 40.12 and 21.34%). In thrombolytic assessments, all the leaves extract showed moderate (considering > 20% moderate; **p < 0.01; *p < 0.05) clot lysis activity, but among the extracts, Uncaria cordata (27.36 ± 0.10%) showed the highest and Stachytarpheta indica the lowest (6.14 ± 0.20%) percent clot lysis as compared with the standard streptokinase (65.15 ± 0.16%).
Conclusion:
This study was conducted to support the folkloric use of seven medicinal plant leaves to improve cancer or myocardial infarction.
Although, it is a preliminary screening but it will be targeted to the scientific community to put more attention and to do an extensive and molecular level study in future. |
| first_indexed | 2025-11-14T17:16:14Z |
| format | Proceeding Paper |
| id | iium-68159 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T17:16:14Z |
| publishDate | 2018 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-681592022-11-07T03:46:56Z http://irep.iium.edu.my/68159/ Cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract Azad, A.K. Sarker, Md. Zaidul Islam Helal Uddin, A.B.M. Alaama, Mohamed Abdullah, J. QD Chemistry RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry Background: In practice, either the herbs themselves or the crude extracts are used for traditional remedies but not the isolated molecules. Therefore, the importance of toxicity and clot lysis study of the crude extract is beyond description. Objective: The aim of our study was to find out the preliminary cytotoxic and thrombolytic effect of the seven selected medicinal plant leaves extract. Methods: Cold maceration was performed using ethanol for extraction purposes. The cytotoxicity study was done by using brine shrimp nauplii (Artemia salina) and MTT assays by using MCF-7 cell line. Result: In the cytotoxic activity, out of the seven leaves extract three of them exhibited stronger brine shrimp lethality with 4 TH PHARMACEUTICAL RESEARCH CONFERENCE 2018 Challenging The Inquisitive Minds: Frontier Of The Future 24 – 26 August 2018 | Cyberjaya, Malaysia LC50 122.548 (Uncaria acida), 170.861 (Leea indica) and 175.469 (Piper porphyrophyllum) μg /mL respectively, and on MCF-7 cell line, they also exhibited moderate cytotoxic activity with different concentration of the extract of the same plant leaves such as, Uncaria acida (72.31, 56.22, 38.12 and 9.24%), Leea indica (67.31, 58.22, 43.12 and 15.24%), Piper porphyrophyllum (65.88, 48.12, 40.12 and 21.34%). In thrombolytic assessments, all the leaves extract showed moderate (considering > 20% moderate; **p < 0.01; *p < 0.05) clot lysis activity, but among the extracts, Uncaria cordata (27.36 ± 0.10%) showed the highest and Stachytarpheta indica the lowest (6.14 ± 0.20%) percent clot lysis as compared with the standard streptokinase (65.15 ± 0.16%). Conclusion: This study was conducted to support the folkloric use of seven medicinal plant leaves to improve cancer or myocardial infarction. Although, it is a preliminary screening but it will be targeted to the scientific community to put more attention and to do an extensive and molecular level study in future. 2018 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/68159/1/IPRC-2018-1.pdf Azad, A.K. and Sarker, Md. Zaidul Islam and Helal Uddin, A.B.M. and Alaama, Mohamed and Abdullah, J. (2018) Cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract. In: 4th International Pharmaceutical Research Conference 2018, 24-26 August 2018, Cyberjaya. (Unpublished) |
| spellingShingle | QD Chemistry RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry Azad, A.K. Sarker, Md. Zaidul Islam Helal Uddin, A.B.M. Alaama, Mohamed Abdullah, J. Cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract |
| title | Cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract |
| title_full | Cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract |
| title_fullStr | Cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract |
| title_full_unstemmed | Cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract |
| title_short | Cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract |
| title_sort | cytotoxicity and thrombolytic potential of seven different medicinal plant leaves extract |
| topic | QD Chemistry RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry |
| url | http://irep.iium.edu.my/68159/ http://irep.iium.edu.my/68159/1/IPRC-2018-1.pdf |