Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology
Sago wastewater (SWW) causes pollution to the environment due to its high organic content. Annually, about 2.5 million tons of SWW is produced in Malaysia. In this study, the potential of SWW as a substrate for biohydrogen production by Enterobacter aerogenes (E. aerogenes) was evaluated. Response S...
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| Format: | Article |
| Language: | English English English |
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Elsevier
2018
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| Online Access: | http://irep.iium.edu.my/67867/ http://irep.iium.edu.my/67867/1/Published%20article.pdf http://irep.iium.edu.my/67867/13/67867_Optimization%20of%20culture%20conditions%20for%20biohydrogen%20production%20from%20sago%20wastewater_scopus.pdf http://irep.iium.edu.my/67867/18/67867%20Optimization%20of%20culture%20conditions%20for%20biohydrogen%20production%20WOS.pdf |
| _version_ | 1848786841887571968 |
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| author | Ulhiza, Tami Astie Mohamad Puad, Noor Illi Azmi, Azlin Suhaida |
| author_facet | Ulhiza, Tami Astie Mohamad Puad, Noor Illi Azmi, Azlin Suhaida |
| author_sort | Ulhiza, Tami Astie |
| building | IIUM Repository |
| collection | Online Access |
| description | Sago wastewater (SWW) causes pollution to the environment due to its high organic content. Annually, about 2.5 million tons of SWW is produced in Malaysia. In this study, the potential of SWW as a substrate for biohydrogen production by Enterobacter aerogenes (E. aerogenes) was evaluated. Response Surface Methodology (RSM) was employed to find the optimum conditions. From preliminary optimization, it was found that the most significant factors were yeast extract, temperature, and inoculum size. According to Face Centered Central Composite Design (FCCCD), the maximum hydrogen concentration and yield were 630.67 μmol/L and 7.42 mmol H2/mol glucose, respectively, which is obtained from the sample supplemented with 4.8 g/L yeast extract concentration, 5% inoculum, and incubated at the temperature of 31 °C. Cumulative hydrogen production curve fitted by the modified Gompertz equation suggested that Hmax, Rmax, and λ from this study were 15.10 mL, 2.18 mL/h, and 9.84 h, respectively. |
| first_indexed | 2025-11-14T17:15:27Z |
| format | Article |
| id | iium-67867 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:15:27Z |
| publishDate | 2018 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-678672019-01-27T06:46:43Z http://irep.iium.edu.my/67867/ Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology Ulhiza, Tami Astie Mohamad Puad, Noor Illi Azmi, Azlin Suhaida TP248.13 Biotechnology Sago wastewater (SWW) causes pollution to the environment due to its high organic content. Annually, about 2.5 million tons of SWW is produced in Malaysia. In this study, the potential of SWW as a substrate for biohydrogen production by Enterobacter aerogenes (E. aerogenes) was evaluated. Response Surface Methodology (RSM) was employed to find the optimum conditions. From preliminary optimization, it was found that the most significant factors were yeast extract, temperature, and inoculum size. According to Face Centered Central Composite Design (FCCCD), the maximum hydrogen concentration and yield were 630.67 μmol/L and 7.42 mmol H2/mol glucose, respectively, which is obtained from the sample supplemented with 4.8 g/L yeast extract concentration, 5% inoculum, and incubated at the temperature of 31 °C. Cumulative hydrogen production curve fitted by the modified Gompertz equation suggested that Hmax, Rmax, and λ from this study were 15.10 mL, 2.18 mL/h, and 9.84 h, respectively. Elsevier 2018-10-31 Article PeerReviewed application/pdf en http://irep.iium.edu.my/67867/1/Published%20article.pdf application/pdf en http://irep.iium.edu.my/67867/13/67867_Optimization%20of%20culture%20conditions%20for%20biohydrogen%20production%20from%20sago%20wastewater_scopus.pdf application/pdf en http://irep.iium.edu.my/67867/18/67867%20Optimization%20of%20culture%20conditions%20for%20biohydrogen%20production%20WOS.pdf Ulhiza, Tami Astie and Mohamad Puad, Noor Illi and Azmi, Azlin Suhaida (2018) Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology. International Journal of Hydrogen Energy, 43 (49). pp. 22148-22158. ISSN 0360-3199 https://www.sciencedirect.com/science/article/pii/S0360319918332348?dgcid=author 10.1016/j.ijhydene.2018.10.057 |
| spellingShingle | TP248.13 Biotechnology Ulhiza, Tami Astie Mohamad Puad, Noor Illi Azmi, Azlin Suhaida Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology |
| title | Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology |
| title_full | Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology |
| title_fullStr | Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology |
| title_full_unstemmed | Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology |
| title_short | Optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology |
| title_sort | optimization of culture conditions for biohydrogen production from sago wastewater by enterobacter aerogenes using response surface methodology |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/67867/ http://irep.iium.edu.my/67867/ http://irep.iium.edu.my/67867/ http://irep.iium.edu.my/67867/1/Published%20article.pdf http://irep.iium.edu.my/67867/13/67867_Optimization%20of%20culture%20conditions%20for%20biohydrogen%20production%20from%20sago%20wastewater_scopus.pdf http://irep.iium.edu.my/67867/18/67867%20Optimization%20of%20culture%20conditions%20for%20biohydrogen%20production%20WOS.pdf |