The spectrophotometric determination of visual spectral sensitivity of sutchi catfish Pangasianodon hypophthalmus
Visual spectral sensitivity refers to the relative sensitivity of the eye towards different wavelengths of light. In fish, their eyes show specific adaptation in sensitivity to match the different light environments in their natural habitats. Sutchi catfish Pangasianodon hypophthalmus is one of the...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/67517/ http://irep.iium.edu.my/67517/1/67517_The%20spectrophotometric%20determination%20of%20visual.pdf |
| _version_ | 1848786771118129152 |
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| author | Nai, Han Tan Mukai, Yukinori Okawa, Ryo Anraku, Kazuhiko |
| author_facet | Nai, Han Tan Mukai, Yukinori Okawa, Ryo Anraku, Kazuhiko |
| author_sort | Nai, Han Tan |
| building | IIUM Repository |
| collection | Online Access |
| description | Visual spectral sensitivity refers to the relative sensitivity of the eye towards different wavelengths of light. In fish, their eyes show specific
adaptation in sensitivity to match the different light environments in their natural habitats. Sutchi catfish Pangasianodon hypophthalmus is one of the
commercially cultured freshwater fish species in Southeast Asia. The present study aimed to understand the visual spectral sensitivity of sutchi
catfish through spectrophotometric analyses of the spectral absorbance of retinal wholemounts for the goal to establish appropriate lighting
conditions in aquaculture system to improve growth performance of sutchi catfish. In the dark room, the retinae of sutchi catfish were isolated under
a dissecting microscope with the help of a night vision goggles. The isolated retinae were cut into approximately 9 mm2
-size pieces. Each retina
sample was then placed on a coverslip with a drop of saline solution and covered with another coverslip. Spectral absorbance of retinal
wholemounts was measured by means of a portable modular spectrophotometer. A broad wavelength LED was used as the main light source,
while blue (peak at 454 nm), green (520 nm), yellow (590 nm) and red (632 nm) LEDs were used as the supplementary light sources for flashing.
Spectrophotometric measurement data were used to generate spectral absorbance curves. Comparison between the peaks of the curves showed
that red light exposure induced a prominent shift of peak spectral absorbance towards shorter light wavelengths, suggesting the eyes of sutchi
catfish had spectral sensitivity for light wavelength of red. |
| first_indexed | 2025-11-14T17:14:20Z |
| format | Proceeding Paper |
| id | iium-67517 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T17:14:20Z |
| publishDate | 2018 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-675172018-11-27T04:07:33Z http://irep.iium.edu.my/67517/ The spectrophotometric determination of visual spectral sensitivity of sutchi catfish Pangasianodon hypophthalmus Nai, Han Tan Mukai, Yukinori Okawa, Ryo Anraku, Kazuhiko SH Aquaculture. Fisheries. Angling SH151 Aquaculture - Fish Culture Visual spectral sensitivity refers to the relative sensitivity of the eye towards different wavelengths of light. In fish, their eyes show specific adaptation in sensitivity to match the different light environments in their natural habitats. Sutchi catfish Pangasianodon hypophthalmus is one of the commercially cultured freshwater fish species in Southeast Asia. The present study aimed to understand the visual spectral sensitivity of sutchi catfish through spectrophotometric analyses of the spectral absorbance of retinal wholemounts for the goal to establish appropriate lighting conditions in aquaculture system to improve growth performance of sutchi catfish. In the dark room, the retinae of sutchi catfish were isolated under a dissecting microscope with the help of a night vision goggles. The isolated retinae were cut into approximately 9 mm2 -size pieces. Each retina sample was then placed on a coverslip with a drop of saline solution and covered with another coverslip. Spectral absorbance of retinal wholemounts was measured by means of a portable modular spectrophotometer. A broad wavelength LED was used as the main light source, while blue (peak at 454 nm), green (520 nm), yellow (590 nm) and red (632 nm) LEDs were used as the supplementary light sources for flashing. Spectrophotometric measurement data were used to generate spectral absorbance curves. Comparison between the peaks of the curves showed that red light exposure induced a prominent shift of peak spectral absorbance towards shorter light wavelengths, suggesting the eyes of sutchi catfish had spectral sensitivity for light wavelength of red. 2018-10 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/67517/1/67517_The%20spectrophotometric%20determination%20of%20visual.pdf Nai, Han Tan and Mukai, Yukinori and Okawa, Ryo and Anraku, Kazuhiko (2018) The spectrophotometric determination of visual spectral sensitivity of sutchi catfish Pangasianodon hypophthalmus. In: International Conference on Agriculture, Animal Sciences and Food Technology (ICAFT) 2018, 30th-31st Oct. 2018, Kuala Terengganu, Terengganu. https://www.unisza.edu.my/icaft2018/index.php?lang=en |
| spellingShingle | SH Aquaculture. Fisheries. Angling SH151 Aquaculture - Fish Culture Nai, Han Tan Mukai, Yukinori Okawa, Ryo Anraku, Kazuhiko The spectrophotometric determination of visual spectral sensitivity of sutchi catfish Pangasianodon hypophthalmus |
| title | The spectrophotometric determination of visual spectral sensitivity of sutchi catfish Pangasianodon hypophthalmus |
| title_full | The spectrophotometric determination of visual spectral sensitivity of sutchi catfish Pangasianodon hypophthalmus |
| title_fullStr | The spectrophotometric determination of visual spectral sensitivity of sutchi catfish Pangasianodon hypophthalmus |
| title_full_unstemmed | The spectrophotometric determination of visual spectral sensitivity of sutchi catfish Pangasianodon hypophthalmus |
| title_short | The spectrophotometric determination of visual spectral sensitivity of sutchi catfish Pangasianodon hypophthalmus |
| title_sort | spectrophotometric determination of visual spectral sensitivity of sutchi catfish pangasianodon hypophthalmus |
| topic | SH Aquaculture. Fisheries. Angling SH151 Aquaculture - Fish Culture |
| url | http://irep.iium.edu.my/67517/ http://irep.iium.edu.my/67517/ http://irep.iium.edu.my/67517/1/67517_The%20spectrophotometric%20determination%20of%20visual.pdf |