Antioxidant activities of Dialium indum L. fruit and gas chromatography-mass spectrometry (GC-MS) of the active fractions
The fruit of Dialium indum L. (Fabaceae) is one of the edible wild fruits native to Southeast Asia. The mesocarp is consumed as sweets while the exocarp and seed are regarded as waste. This study aimed to evaluate the antioxidant activities of the fruit by using four assays, which measure its capabi...
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| Format: | Article |
| Language: | English English English |
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Multidisciplinary Digital Publishing Institute (MDPI)
2018
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| Online Access: | http://irep.iium.edu.my/67382/ http://irep.iium.edu.my/67382/1/67382_Antioxidant%20activities%20of%20Dialium%20indum%20L.%20fruit.pdf http://irep.iium.edu.my/67382/2/67382_Antioxidant%20activities%20of%20Dialium%20indum%20L.%20fruit_SCOPUS.pdf http://irep.iium.edu.my/67382/13/67382_Antioxidant%20activities%20of%20Dialium%20indum%20L.%20fruit_WOS.pdf |
| _version_ | 1848786749294116864 |
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| author | Osman, Muhamad Faris Mohd Hassan, Norazian Khatib, Alfi Tolos, Siti Marponga |
| author_facet | Osman, Muhamad Faris Mohd Hassan, Norazian Khatib, Alfi Tolos, Siti Marponga |
| author_sort | Osman, Muhamad Faris |
| building | IIUM Repository |
| collection | Online Access |
| description | The fruit of Dialium indum L. (Fabaceae) is one of the edible wild fruits native to Southeast Asia. The mesocarp is consumed as sweets while the exocarp and seed are regarded as waste. This study aimed to evaluate the antioxidant activities of the fruit by using four assays, which measure its capabilities in reducing phosphomolybdic-phosphotungstic acid reagents, neocuproine, 2,2-diphenyl-picrylhydrazyl (DPPH), and inhibiting linoleic acid peroxidation. The active fractions were then analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that the seed methanol fraction (SMF) exhibited the strongest antioxidant activity with significantly higher (p < 0.05) gallic acid equivalence (GAE), total antioxidant capacity (TAC), and DPPH radical scavenging activity (IC50 31.71; 0.88 μg/mL) than the other fractions. The exocarp dichloromethane fraction (EDF) was the discriminating fraction by having remarkable linoleic acid peroxidation inhibition (IC50 121.43; 2.97 μg/mL). A total of thirty-eight metabolites were detected in derivatized EDF and SMF with distinctive classes of phenolics and amino acids, respectively. Bioautography-guided fractionation of EDF afforded five antioxidant-enriched subfractions with four other detected phenolics. The results revealed the antioxidant properties of D. indum fruit, which has potential benefits in pharmaceutical, nutraceutical, and cosmeceutical applications. |
| first_indexed | 2025-11-14T17:13:59Z |
| format | Article |
| id | iium-67382 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:13:59Z |
| publishDate | 2018 |
| publisher | Multidisciplinary Digital Publishing Institute (MDPI) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-673822018-12-31T12:37:46Z http://irep.iium.edu.my/67382/ Antioxidant activities of Dialium indum L. fruit and gas chromatography-mass spectrometry (GC-MS) of the active fractions Osman, Muhamad Faris Mohd Hassan, Norazian Khatib, Alfi Tolos, Siti Marponga RS403 Materia Medica-Pharmaceutical Chemistry The fruit of Dialium indum L. (Fabaceae) is one of the edible wild fruits native to Southeast Asia. The mesocarp is consumed as sweets while the exocarp and seed are regarded as waste. This study aimed to evaluate the antioxidant activities of the fruit by using four assays, which measure its capabilities in reducing phosphomolybdic-phosphotungstic acid reagents, neocuproine, 2,2-diphenyl-picrylhydrazyl (DPPH), and inhibiting linoleic acid peroxidation. The active fractions were then analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that the seed methanol fraction (SMF) exhibited the strongest antioxidant activity with significantly higher (p < 0.05) gallic acid equivalence (GAE), total antioxidant capacity (TAC), and DPPH radical scavenging activity (IC50 31.71; 0.88 μg/mL) than the other fractions. The exocarp dichloromethane fraction (EDF) was the discriminating fraction by having remarkable linoleic acid peroxidation inhibition (IC50 121.43; 2.97 μg/mL). A total of thirty-eight metabolites were detected in derivatized EDF and SMF with distinctive classes of phenolics and amino acids, respectively. Bioautography-guided fractionation of EDF afforded five antioxidant-enriched subfractions with four other detected phenolics. The results revealed the antioxidant properties of D. indum fruit, which has potential benefits in pharmaceutical, nutraceutical, and cosmeceutical applications. Multidisciplinary Digital Publishing Institute (MDPI) 2018-11 Article PeerReviewed application/pdf en http://irep.iium.edu.my/67382/1/67382_Antioxidant%20activities%20of%20Dialium%20indum%20L.%20fruit.pdf application/pdf en http://irep.iium.edu.my/67382/2/67382_Antioxidant%20activities%20of%20Dialium%20indum%20L.%20fruit_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/67382/13/67382_Antioxidant%20activities%20of%20Dialium%20indum%20L.%20fruit_WOS.pdf Osman, Muhamad Faris and Mohd Hassan, Norazian and Khatib, Alfi and Tolos, Siti Marponga (2018) Antioxidant activities of Dialium indum L. fruit and gas chromatography-mass spectrometry (GC-MS) of the active fractions. Antioxidants, 7 (11). pp. 1-14. ISSN 2076-3921 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262551/ 10.3390/antiox7110154 |
| spellingShingle | RS403 Materia Medica-Pharmaceutical Chemistry Osman, Muhamad Faris Mohd Hassan, Norazian Khatib, Alfi Tolos, Siti Marponga Antioxidant activities of Dialium indum L. fruit and gas chromatography-mass spectrometry (GC-MS) of the active fractions |
| title | Antioxidant activities of Dialium indum L. fruit and gas chromatography-mass spectrometry (GC-MS) of the active fractions |
| title_full | Antioxidant activities of Dialium indum L. fruit and gas chromatography-mass spectrometry (GC-MS) of the active fractions |
| title_fullStr | Antioxidant activities of Dialium indum L. fruit and gas chromatography-mass spectrometry (GC-MS) of the active fractions |
| title_full_unstemmed | Antioxidant activities of Dialium indum L. fruit and gas chromatography-mass spectrometry (GC-MS) of the active fractions |
| title_short | Antioxidant activities of Dialium indum L. fruit and gas chromatography-mass spectrometry (GC-MS) of the active fractions |
| title_sort | antioxidant activities of dialium indum l. fruit and gas chromatography-mass spectrometry (gc-ms) of the active fractions |
| topic | RS403 Materia Medica-Pharmaceutical Chemistry |
| url | http://irep.iium.edu.my/67382/ http://irep.iium.edu.my/67382/ http://irep.iium.edu.my/67382/ http://irep.iium.edu.my/67382/1/67382_Antioxidant%20activities%20of%20Dialium%20indum%20L.%20fruit.pdf http://irep.iium.edu.my/67382/2/67382_Antioxidant%20activities%20of%20Dialium%20indum%20L.%20fruit_SCOPUS.pdf http://irep.iium.edu.my/67382/13/67382_Antioxidant%20activities%20of%20Dialium%20indum%20L.%20fruit_WOS.pdf |