Factors affecting the hydrolysis of empty fruit bunches in ionic liquid-compatible cellulase system

Utilization of the abundant raw materials in Malaysia as well as surmounting the obstacle of the shortcoming of fossil fuels resources are current concerns of the research. Cellulase development aims to enhance the bioethanol production from lignocellulosic biomass such as empty fruit bunches (EFB)...

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Main Authors: Elgharbawy, Amal A.M., Alam, Md. Zahangir
Format: Proceeding Paper
Language:English
Published: Kulliyyah of Engineering, International Islamic University Malaysia 2018
Subjects:
Online Access:http://irep.iium.edu.my/67239/
http://irep.iium.edu.my/67239/1/67239_Factors%20Affecting%20the%20Hydrolysis.pdf
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author Elgharbawy, Amal A.M.
Alam, Md. Zahangir
author_facet Elgharbawy, Amal A.M.
Alam, Md. Zahangir
author_sort Elgharbawy, Amal A.M.
building IIUM Repository
collection Online Access
description Utilization of the abundant raw materials in Malaysia as well as surmounting the obstacle of the shortcoming of fossil fuels resources are current concerns of the research. Cellulase development aims to enhance the bioethanol production from lignocellulosic biomass such as empty fruit bunches (EFB) that resists enzymatic hydrolysis due to its complex structure. Hence, ionic liquids (ILs) have been introduced with the ability to disrupt the lignocellulose to be accessible by enzymes and thus it is expected to achieve a significant improvement by conducting both treatment and hydrolysis in one step by leaping cellulose regeneration. Palm kernel cake (PKC) was utilized as the primary media for cellulase production through solid-state bioconversion (SSB) by Trichoderma reesei. Factors that affect the hydrolysis in the ionic liquid-cellulase (IL-E) system was investigated using one-factor at a time (OFAT) and reducing sugar obtained as the response to the study. It was found that the biomass could be loaded up to 35% (w/v) and agitation has influenced the rate of reducing sugar production where increasing the speed was associated with higher concentration. Higher IL concentration in the combined system caused lower enzymatic activity and hence, less sugar was produced. The results obtained can be employed for the one-step process in the conversion of EFB into fermentable sugar.
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format Proceeding Paper
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institution International Islamic University Malaysia
institution_category Local University
language English
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publishDate 2018
publisher Kulliyyah of Engineering, International Islamic University Malaysia
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spelling iium-672392018-11-16T04:47:51Z http://irep.iium.edu.my/67239/ Factors affecting the hydrolysis of empty fruit bunches in ionic liquid-compatible cellulase system Elgharbawy, Amal A.M. Alam, Md. Zahangir TP248.13 Biotechnology Utilization of the abundant raw materials in Malaysia as well as surmounting the obstacle of the shortcoming of fossil fuels resources are current concerns of the research. Cellulase development aims to enhance the bioethanol production from lignocellulosic biomass such as empty fruit bunches (EFB) that resists enzymatic hydrolysis due to its complex structure. Hence, ionic liquids (ILs) have been introduced with the ability to disrupt the lignocellulose to be accessible by enzymes and thus it is expected to achieve a significant improvement by conducting both treatment and hydrolysis in one step by leaping cellulose regeneration. Palm kernel cake (PKC) was utilized as the primary media for cellulase production through solid-state bioconversion (SSB) by Trichoderma reesei. Factors that affect the hydrolysis in the ionic liquid-cellulase (IL-E) system was investigated using one-factor at a time (OFAT) and reducing sugar obtained as the response to the study. It was found that the biomass could be loaded up to 35% (w/v) and agitation has influenced the rate of reducing sugar production where increasing the speed was associated with higher concentration. Higher IL concentration in the combined system caused lower enzymatic activity and hence, less sugar was produced. The results obtained can be employed for the one-step process in the conversion of EFB into fermentable sugar. Kulliyyah of Engineering, International Islamic University Malaysia 2018-09-19 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/67239/1/67239_Factors%20Affecting%20the%20Hydrolysis.pdf Elgharbawy, Amal A.M. and Alam, Md. Zahangir (2018) Factors affecting the hydrolysis of empty fruit bunches in ionic liquid-compatible cellulase system. In: 5th International Conference on Biotechnology Engineering (ICBioE 2018), 19th-20th September 2018, Kuala Lumpur. http://www.iium.edu.my/icbioe/2018/
spellingShingle TP248.13 Biotechnology
Elgharbawy, Amal A.M.
Alam, Md. Zahangir
Factors affecting the hydrolysis of empty fruit bunches in ionic liquid-compatible cellulase system
title Factors affecting the hydrolysis of empty fruit bunches in ionic liquid-compatible cellulase system
title_full Factors affecting the hydrolysis of empty fruit bunches in ionic liquid-compatible cellulase system
title_fullStr Factors affecting the hydrolysis of empty fruit bunches in ionic liquid-compatible cellulase system
title_full_unstemmed Factors affecting the hydrolysis of empty fruit bunches in ionic liquid-compatible cellulase system
title_short Factors affecting the hydrolysis of empty fruit bunches in ionic liquid-compatible cellulase system
title_sort factors affecting the hydrolysis of empty fruit bunches in ionic liquid-compatible cellulase system
topic TP248.13 Biotechnology
url http://irep.iium.edu.my/67239/
http://irep.iium.edu.my/67239/
http://irep.iium.edu.my/67239/1/67239_Factors%20Affecting%20the%20Hydrolysis.pdf