Application of retina acid improves form and function of tissue engineered corneal construct
Retinoic acid has recently been shown to control the phenotype and extracellular matrix composition of corneal stromal cells cultured in vitro as monolayers. This study set out to investigate the effects of retinoic acid on human corneal keratocytes within a 3D environment. Human corneal keratocytes...
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Taylor and Francis
2015
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| Online Access: | http://irep.iium.edu.my/67141/ http://irep.iium.edu.my/67141/1/Application%20of%20retinoic%20acid%20improves%20form%20and%20function%20of%20tissue%20engineered%20corneal%20construct.pdf http://irep.iium.edu.my/67141/7/Scopus%20-%20Application%20of%20retinoic%20acid.pdf http://irep.iium.edu.my/67141/8/WOS%20-%20Application%20of%20retinoic%20acid.pdf |
| _version_ | 1848786705596809216 |
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| author | Zainal Abidin, Fadhilah Gouveia, Ricardo M Connon, Che J |
| author_facet | Zainal Abidin, Fadhilah Gouveia, Ricardo M Connon, Che J |
| author_sort | Zainal Abidin, Fadhilah |
| building | IIUM Repository |
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| description | Retinoic acid has recently been shown to control the phenotype and extracellular matrix composition of corneal stromal cells cultured in vitro as monolayers. This study set out to investigate the effects of retinoic acid on human corneal keratocytes within a 3D environment. Human corneal keratocytes were encapsulated in collagen gels, which were subsequently compressed under load, and cultured in serum-free media supplemented with 10 mM retinoic acid or DMSO vehicle for 30 days. Cell proliferation was quantified on selected days, while the expression of several important keratocytes markers was evaluated at day 30 using RT-PCR and immunoblotting. The weight and size of the collagen constructs were measured before and after hydration and contraction analyses. Retinoic acid enhanced keratocyte proliferation until day 30, whereas cells in control culture conditions showed reduced numbers after day 21. Both gene and protein expressions of keratocyte-characteristic proteoglycans (keratocan, lumican and decorin), corneal crystallins and collagen type I and V were significantly increased following retinoic acid supplementation. Retinoic acid also significantly reduced the expression of matrix metalloproteases 1, 3 and 9 while not increasing a-smooth muscle actin and fibronectin expression. Furthermore, these effects were also correlated with the ability of retinoic acid to significantly inhibit the contractility of keratocytes while allowing the build-up of corneal stromal extracellular matrix within the 3D constructs. Thus, retinoic acid supplementation represents a promising strategy to improve the phenotype of 3D-cultured keratocytes, and their usefulness as a model of corneal stroma for corneal biology and regenerative medicine applications. |
| first_indexed | 2025-11-14T17:13:17Z |
| format | Article |
| id | iium-67141 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:13:17Z |
| publishDate | 2015 |
| publisher | Taylor and Francis |
| recordtype | eprints |
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| spelling | iium-671412018-12-14T03:14:32Z http://irep.iium.edu.my/67141/ Application of retina acid improves form and function of tissue engineered corneal construct Zainal Abidin, Fadhilah Gouveia, Ricardo M Connon, Che J RE Ophthalmology Retinoic acid has recently been shown to control the phenotype and extracellular matrix composition of corneal stromal cells cultured in vitro as monolayers. This study set out to investigate the effects of retinoic acid on human corneal keratocytes within a 3D environment. Human corneal keratocytes were encapsulated in collagen gels, which were subsequently compressed under load, and cultured in serum-free media supplemented with 10 mM retinoic acid or DMSO vehicle for 30 days. Cell proliferation was quantified on selected days, while the expression of several important keratocytes markers was evaluated at day 30 using RT-PCR and immunoblotting. The weight and size of the collagen constructs were measured before and after hydration and contraction analyses. Retinoic acid enhanced keratocyte proliferation until day 30, whereas cells in control culture conditions showed reduced numbers after day 21. Both gene and protein expressions of keratocyte-characteristic proteoglycans (keratocan, lumican and decorin), corneal crystallins and collagen type I and V were significantly increased following retinoic acid supplementation. Retinoic acid also significantly reduced the expression of matrix metalloproteases 1, 3 and 9 while not increasing a-smooth muscle actin and fibronectin expression. Furthermore, these effects were also correlated with the ability of retinoic acid to significantly inhibit the contractility of keratocytes while allowing the build-up of corneal stromal extracellular matrix within the 3D constructs. Thus, retinoic acid supplementation represents a promising strategy to improve the phenotype of 3D-cultured keratocytes, and their usefulness as a model of corneal stroma for corneal biology and regenerative medicine applications. Taylor and Francis 2015-10-26 Article PeerReviewed application/pdf en http://irep.iium.edu.my/67141/1/Application%20of%20retinoic%20acid%20improves%20form%20and%20function%20of%20tissue%20engineered%20corneal%20construct.pdf application/pdf en http://irep.iium.edu.my/67141/7/Scopus%20-%20Application%20of%20retinoic%20acid.pdf application/pdf en http://irep.iium.edu.my/67141/8/WOS%20-%20Application%20of%20retinoic%20acid.pdf Zainal Abidin, Fadhilah and Gouveia, Ricardo M and Connon, Che J (2015) Application of retina acid improves form and function of tissue engineered corneal construct. Organogenesis, 11 (3). pp. 122-136. ISSN 1547-6278 E-ISSN 1555-8592 https://www.tandfonline.com/doi/full/10.1080/15476278.2015.1093267 10.1080/15476278.2015.1093267 |
| spellingShingle | RE Ophthalmology Zainal Abidin, Fadhilah Gouveia, Ricardo M Connon, Che J Application of retina acid improves form and function of tissue engineered corneal construct |
| title | Application of retina acid improves form and function of tissue engineered corneal construct |
| title_full | Application of retina acid improves form and function of tissue engineered corneal construct |
| title_fullStr | Application of retina acid improves form and function of tissue engineered corneal construct |
| title_full_unstemmed | Application of retina acid improves form and function of tissue engineered corneal construct |
| title_short | Application of retina acid improves form and function of tissue engineered corneal construct |
| title_sort | application of retina acid improves form and function of tissue engineered corneal construct |
| topic | RE Ophthalmology |
| url | http://irep.iium.edu.my/67141/ http://irep.iium.edu.my/67141/ http://irep.iium.edu.my/67141/ http://irep.iium.edu.my/67141/1/Application%20of%20retinoic%20acid%20improves%20form%20and%20function%20of%20tissue%20engineered%20corneal%20construct.pdf http://irep.iium.edu.my/67141/7/Scopus%20-%20Application%20of%20retinoic%20acid.pdf http://irep.iium.edu.my/67141/8/WOS%20-%20Application%20of%20retinoic%20acid.pdf |