Naringin attenuates the development of carrageenan-induced acute lung inflammation through inhibition of NF-κb, STAT3 and pro-inflammatory mediators and enhancement of IκBα and anti-inflammatory cytokines
Naringin has been reported to possess diverse pharmacological properties, including anti-arthritic and anti-inflammatory activities. The aim of the present study was to determine the potential anti-inflammatory effect of naringin in a mouse model of carrageenan-induced pleurisy. A single dose of nar...
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| Format: | Article |
| Language: | English English |
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Springer Nature Switzerland AG.
2015
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| Online Access: | http://irep.iium.edu.my/66957/ http://irep.iium.edu.my/66957/1/Naringin%20Attenuates%20the%20Development%20of%20Carrageenan-Induced%20Acute%20Lung%20Inflammation%20Through%20Inhibition%20of%20NF-%CE%BAb%2C%20STAT3%20and%20Pro-Inflammatory%20Mediators%20and%20Enhancement%20of%20I%CE%BAB%CE%B1%20and%20Anti-Inflammatory%20Cytokines.pdf http://irep.iium.edu.my/66957/7/66957_Scopus%20-%20Document%20details.pdf |
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| author | Ahmad, Sheikh Fayaz Attia, Sabry M. Bakheet, Saleh A. Zoheir, Khairy M. A. Ansari, Mushtaq Ahmad Korashy, Hesham M. Abdel-Hamied, Hala E. Ashour, Abdelkader Elbadawy Abbas Abd-Allah, Adel R. A. |
| author_facet | Ahmad, Sheikh Fayaz Attia, Sabry M. Bakheet, Saleh A. Zoheir, Khairy M. A. Ansari, Mushtaq Ahmad Korashy, Hesham M. Abdel-Hamied, Hala E. Ashour, Abdelkader Elbadawy Abbas Abd-Allah, Adel R. A. |
| author_sort | Ahmad, Sheikh Fayaz |
| building | IIUM Repository |
| collection | Online Access |
| description | Naringin has been reported to possess diverse pharmacological properties, including anti-arthritic and anti-inflammatory activities. The aim of the present study was to determine the potential anti-inflammatory effect of naringin in a mouse model of carrageenan-induced pleurisy. A single dose of naringin (40 and 80 mg/kg) was administered per oral (p.o.) 1 h before carrageenan (Cg) administration. Pro- and anti-inflammatory cytokines were analysed in pleural fluid. We also assessed the effects of naringin on the expression levels of iNOS, inducible cyclooxygenase isoform (COX-2), ICAM-1, MIP-2, PGE2, STAT3, TGF-β1, nuclear factor kappa B (NF-κB) and inhibitor of kappa B (IκBα) in lung tissue. The histological examinations revealed anti-inflammatory effect of naringin while Cg group deteriorated. Naringin downregulated Th1 and upregulated Th2 cytokines. Western blot analyses revealed increased protein expression of NF-κB, STAT3 and COX-2 and decreased IκBα in response to Cg treatment, which were reversed by the treatment with naringin. In the Cg group, mRNA expression levels of pro-inflammatory mediators upregulated and anti-inflammatory mediators downregulated. Naringin reversed these actions. |
| first_indexed | 2025-11-14T17:12:46Z |
| format | Article |
| id | iium-66957 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:12:46Z |
| publishDate | 2015 |
| publisher | Springer Nature Switzerland AG. |
| recordtype | eprints |
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| spelling | iium-669572019-01-06T07:57:08Z http://irep.iium.edu.my/66957/ Naringin attenuates the development of carrageenan-induced acute lung inflammation through inhibition of NF-κb, STAT3 and pro-inflammatory mediators and enhancement of IκBα and anti-inflammatory cytokines Ahmad, Sheikh Fayaz Attia, Sabry M. Bakheet, Saleh A. Zoheir, Khairy M. A. Ansari, Mushtaq Ahmad Korashy, Hesham M. Abdel-Hamied, Hala E. Ashour, Abdelkader Elbadawy Abbas Abd-Allah, Adel R. A. RM300 Drugs and their action Naringin has been reported to possess diverse pharmacological properties, including anti-arthritic and anti-inflammatory activities. The aim of the present study was to determine the potential anti-inflammatory effect of naringin in a mouse model of carrageenan-induced pleurisy. A single dose of naringin (40 and 80 mg/kg) was administered per oral (p.o.) 1 h before carrageenan (Cg) administration. Pro- and anti-inflammatory cytokines were analysed in pleural fluid. We also assessed the effects of naringin on the expression levels of iNOS, inducible cyclooxygenase isoform (COX-2), ICAM-1, MIP-2, PGE2, STAT3, TGF-β1, nuclear factor kappa B (NF-κB) and inhibitor of kappa B (IκBα) in lung tissue. The histological examinations revealed anti-inflammatory effect of naringin while Cg group deteriorated. Naringin downregulated Th1 and upregulated Th2 cytokines. Western blot analyses revealed increased protein expression of NF-κB, STAT3 and COX-2 and decreased IκBα in response to Cg treatment, which were reversed by the treatment with naringin. In the Cg group, mRNA expression levels of pro-inflammatory mediators upregulated and anti-inflammatory mediators downregulated. Naringin reversed these actions. Springer Nature Switzerland AG. 2015-04-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/66957/1/Naringin%20Attenuates%20the%20Development%20of%20Carrageenan-Induced%20Acute%20Lung%20Inflammation%20Through%20Inhibition%20of%20NF-%CE%BAb%2C%20STAT3%20and%20Pro-Inflammatory%20Mediators%20and%20Enhancement%20of%20I%CE%BAB%CE%B1%20and%20Anti-Inflammatory%20Cytokines.pdf application/pdf en http://irep.iium.edu.my/66957/7/66957_Scopus%20-%20Document%20details.pdf Ahmad, Sheikh Fayaz and Attia, Sabry M. and Bakheet, Saleh A. and Zoheir, Khairy M. A. and Ansari, Mushtaq Ahmad and Korashy, Hesham M. and Abdel-Hamied, Hala E. and Ashour, Abdelkader Elbadawy Abbas and Abd-Allah, Adel R. A. (2015) Naringin attenuates the development of carrageenan-induced acute lung inflammation through inhibition of NF-κb, STAT3 and pro-inflammatory mediators and enhancement of IκBα and anti-inflammatory cytokines. Inflammation, 38 (2). pp. 846-857. ISSN 0360-3997 E-ISSN 1573-2576 https://link.springer.com/journal/10753 DOI: 10.1007/s10753-014-9994-y |
| spellingShingle | RM300 Drugs and their action Ahmad, Sheikh Fayaz Attia, Sabry M. Bakheet, Saleh A. Zoheir, Khairy M. A. Ansari, Mushtaq Ahmad Korashy, Hesham M. Abdel-Hamied, Hala E. Ashour, Abdelkader Elbadawy Abbas Abd-Allah, Adel R. A. Naringin attenuates the development of carrageenan-induced acute lung inflammation through inhibition of NF-κb, STAT3 and pro-inflammatory mediators and enhancement of IκBα and anti-inflammatory cytokines |
| title | Naringin attenuates the development of carrageenan-induced acute lung inflammation through inhibition of NF-κb, STAT3 and pro-inflammatory mediators and enhancement of IκBα and anti-inflammatory cytokines |
| title_full | Naringin attenuates the development of carrageenan-induced acute lung inflammation through inhibition of NF-κb, STAT3 and pro-inflammatory mediators and enhancement of IκBα and anti-inflammatory cytokines |
| title_fullStr | Naringin attenuates the development of carrageenan-induced acute lung inflammation through inhibition of NF-κb, STAT3 and pro-inflammatory mediators and enhancement of IκBα and anti-inflammatory cytokines |
| title_full_unstemmed | Naringin attenuates the development of carrageenan-induced acute lung inflammation through inhibition of NF-κb, STAT3 and pro-inflammatory mediators and enhancement of IκBα and anti-inflammatory cytokines |
| title_short | Naringin attenuates the development of carrageenan-induced acute lung inflammation through inhibition of NF-κb, STAT3 and pro-inflammatory mediators and enhancement of IκBα and anti-inflammatory cytokines |
| title_sort | naringin attenuates the development of carrageenan-induced acute lung inflammation through inhibition of nf-κb, stat3 and pro-inflammatory mediators and enhancement of iκbα and anti-inflammatory cytokines |
| topic | RM300 Drugs and their action |
| url | http://irep.iium.edu.my/66957/ http://irep.iium.edu.my/66957/ http://irep.iium.edu.my/66957/ http://irep.iium.edu.my/66957/1/Naringin%20Attenuates%20the%20Development%20of%20Carrageenan-Induced%20Acute%20Lung%20Inflammation%20Through%20Inhibition%20of%20NF-%CE%BAb%2C%20STAT3%20and%20Pro-Inflammatory%20Mediators%20and%20Enhancement%20of%20I%CE%BAB%CE%B1%20and%20Anti-Inflammatory%20Cytokines.pdf http://irep.iium.edu.my/66957/7/66957_Scopus%20-%20Document%20details.pdf |