A comparative study of the effects of different low-level lasers on the proliferation, viability, and migration of human melanocytes in vitro.
The aim of this study was to investigate the effects of different low-level laser therapies (LLLTs) of various wavelengths and energies on normal cultured human melanocytes. Various studies have shown the effects of LLLs on various types of cultured cells. Presently, little is known about the biolog...
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Springer London
2015
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| Online Access: | http://irep.iium.edu.my/66889/ http://irep.iium.edu.my/66889/1/A%20comparative%20study%20of%20the%20effects%20of%20different%20low-level%20lasers%20on%20the%20proliferation%2C%20viability%2C%20and%20migration%20of%20human%20melanocytes%20in%20vitro.pdf http://irep.iium.edu.my/66889/8/66889_scopus.pdf |
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| author | M. AlGhamdi, Khalid Kumar, Ashok Ashour, Abdelkader Elbadawy Abbas A. AlGhamdi, Attieh |
| author_facet | M. AlGhamdi, Khalid Kumar, Ashok Ashour, Abdelkader Elbadawy Abbas A. AlGhamdi, Attieh |
| author_sort | M. AlGhamdi, Khalid |
| building | IIUM Repository |
| collection | Online Access |
| description | The aim of this study was to investigate the effects of different low-level laser therapies (LLLTs) of various wavelengths and energies on normal cultured human melanocytes. Various studies have shown the effects of LLLs on various types of cultured cells. Presently, little is known about the biological effects of LLLTs on melanocytes. Melanocytes were exposed to LLLT at 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, or 5.0 J/cm(2) using a blue (457 nm), red (635 nm), or ultraviolet (UV) (355 nm) laser. Melanocyte viability, proliferation, and migration were monitored at 72 h after irradiation. The blue (P < 0.001) and red (P < 0.001 and P < 0.01) lasers significantly enhanced viability at 0.5 to 2.0 J/cm(2), whereas the UV laser (P < 0.001) could significantly enhance viability only at 0.5 and 1.0 J/cm(2) compared with controls. The blue and red lasers also significantly enhanced the proliferation of the melanocytes at 0.5 to 2.0 J/cm(2) (P < 0.001), and the UV laser significantly enhanced proliferation at 0.5 to 1.5 J/cm(2) (P < 0.001 and P < 0.01) compared with controls. The blue laser significantly enhanced melanocyte migration at 0.5 to 4.0 J/cm(2) (P < 0.001 to P < 0.05), but the red (P < 0.001 and P < 0.01) and UV (P < 0.001 to P < 0.05) lasers could significantly enhance such migration at 0.5 to 1.0 J/cm(2) and 0.5 to 2.0 J/cm(2), respectively, compared with controls. LLLT at low energy densities is able to significantly increase melanocyte viability, proliferation, and migration in vitro, and at higher energy densities, it gives non-stimulatory results. Additionally, the blue laser was the best among the three lasers. These findings might have potential application in vitiligo treatment in future. |
| first_indexed | 2025-11-14T17:12:34Z |
| format | Article |
| id | iium-66889 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:12:34Z |
| publishDate | 2015 |
| publisher | Springer London |
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| spelling | iium-668892019-01-04T08:04:40Z http://irep.iium.edu.my/66889/ A comparative study of the effects of different low-level lasers on the proliferation, viability, and migration of human melanocytes in vitro. M. AlGhamdi, Khalid Kumar, Ashok Ashour, Abdelkader Elbadawy Abbas A. AlGhamdi, Attieh RL Dermatology The aim of this study was to investigate the effects of different low-level laser therapies (LLLTs) of various wavelengths and energies on normal cultured human melanocytes. Various studies have shown the effects of LLLs on various types of cultured cells. Presently, little is known about the biological effects of LLLTs on melanocytes. Melanocytes were exposed to LLLT at 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, or 5.0 J/cm(2) using a blue (457 nm), red (635 nm), or ultraviolet (UV) (355 nm) laser. Melanocyte viability, proliferation, and migration were monitored at 72 h after irradiation. The blue (P < 0.001) and red (P < 0.001 and P < 0.01) lasers significantly enhanced viability at 0.5 to 2.0 J/cm(2), whereas the UV laser (P < 0.001) could significantly enhance viability only at 0.5 and 1.0 J/cm(2) compared with controls. The blue and red lasers also significantly enhanced the proliferation of the melanocytes at 0.5 to 2.0 J/cm(2) (P < 0.001), and the UV laser significantly enhanced proliferation at 0.5 to 1.5 J/cm(2) (P < 0.001 and P < 0.01) compared with controls. The blue laser significantly enhanced melanocyte migration at 0.5 to 4.0 J/cm(2) (P < 0.001 to P < 0.05), but the red (P < 0.001 and P < 0.01) and UV (P < 0.001 to P < 0.05) lasers could significantly enhance such migration at 0.5 to 1.0 J/cm(2) and 0.5 to 2.0 J/cm(2), respectively, compared with controls. LLLT at low energy densities is able to significantly increase melanocyte viability, proliferation, and migration in vitro, and at higher energy densities, it gives non-stimulatory results. Additionally, the blue laser was the best among the three lasers. These findings might have potential application in vitiligo treatment in future. Springer London 2015-07 Article PeerReviewed application/pdf en http://irep.iium.edu.my/66889/1/A%20comparative%20study%20of%20the%20effects%20of%20different%20low-level%20lasers%20on%20the%20proliferation%2C%20viability%2C%20and%20migration%20of%20human%20melanocytes%20in%20vitro.pdf application/pdf en http://irep.iium.edu.my/66889/8/66889_scopus.pdf M. AlGhamdi, Khalid and Kumar, Ashok and Ashour, Abdelkader Elbadawy Abbas and A. AlGhamdi, Attieh (2015) A comparative study of the effects of different low-level lasers on the proliferation, viability, and migration of human melanocytes in vitro. Lasers in Medical Science, 30 (5). pp. 1541-1551. ISSN 0268-8921 E-ISSN 1435-604X https://link.springer.com/article/10.1007%2Fs10103-015-1758-x DOI: 10.1007/s10103-015-1758-x |
| spellingShingle | RL Dermatology M. AlGhamdi, Khalid Kumar, Ashok Ashour, Abdelkader Elbadawy Abbas A. AlGhamdi, Attieh A comparative study of the effects of different low-level lasers on the proliferation, viability, and migration of human melanocytes in vitro. |
| title | A comparative study of the effects of different low-level lasers on the proliferation, viability, and migration of human melanocytes in vitro. |
| title_full | A comparative study of the effects of different low-level lasers on the proliferation, viability, and migration of human melanocytes in vitro. |
| title_fullStr | A comparative study of the effects of different low-level lasers on the proliferation, viability, and migration of human melanocytes in vitro. |
| title_full_unstemmed | A comparative study of the effects of different low-level lasers on the proliferation, viability, and migration of human melanocytes in vitro. |
| title_short | A comparative study of the effects of different low-level lasers on the proliferation, viability, and migration of human melanocytes in vitro. |
| title_sort | comparative study of the effects of different low-level lasers on the proliferation, viability, and migration of human melanocytes in vitro. |
| topic | RL Dermatology |
| url | http://irep.iium.edu.my/66889/ http://irep.iium.edu.my/66889/ http://irep.iium.edu.my/66889/ http://irep.iium.edu.my/66889/1/A%20comparative%20study%20of%20the%20effects%20of%20different%20low-level%20lasers%20on%20the%20proliferation%2C%20viability%2C%20and%20migration%20of%20human%20melanocytes%20in%20vitro.pdf http://irep.iium.edu.my/66889/8/66889_scopus.pdf |