ATR-FTIR spectroscopic methods for analysis of black seed oil from alginate beads
Objective: This study aimed to use attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR) for rapid quantification of black seed oil (BSO) from alginate beads and to develop simple analysis method for in vitro release study based on turbidity measurement. Methods: Guluron...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Innovare Academics Sciences Pvt. Ltd
2018
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/66788/ http://irep.iium.edu.my/66788/1/66788_ATR-FTIR%20spectroscopic%20methods.pdf http://irep.iium.edu.my/66788/2/66788_ATR-FTIR%20spectroscopic%20methods_SCOPUS.pdf |
| _version_ | 1848786646132064256 |
|---|---|
| author | Alkhatib, Hamzeh Mohamed, Farahidah Doolaanea, Abd Almonem |
| author_facet | Alkhatib, Hamzeh Mohamed, Farahidah Doolaanea, Abd Almonem |
| author_sort | Alkhatib, Hamzeh |
| building | IIUM Repository |
| collection | Online Access |
| description | Objective: This study aimed to use attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR) for rapid quantification of black seed oil (BSO) from alginate beads and to develop simple analysis method for in vitro release study based on turbidity measurement.
Methods: Guluronic acid-rich (high-G) sodium alginate was used to encapsulate BSO with the aid of Tween 80 as a stabilizer. ATR-FTIR method was developed for quantification of encapsulation efficiency of BSO by applying Beer-Lambert law after selection of a few wave number combinations. UV-vis method based on measurement of emulsion turbidity at 600 nm was also developed to quantify BSO during the releasing from alginate beads in simulated intestine buffer.
Results: ATR-FTIR method exhibited linearity in the range of 25-300 mgBSO/mlemulsion (mgBSO/mlE)with R2=0.998, RSD=8.4%, LOD=0.28 mgBSO/mlE and LOQ=0.87 mgBSO/mlE. BSO-alginate beads was found to completely encapsulate BSO with around 100.5% efficiency. UV-vis method exhibited linearity in the range of 50-300 mgBSO/mlE, R2=0.9931, RSD=1.34%, LOD=0.89 mgBSO/mlE, and LOQ=2.71 mgBSO/mlE. In addition, the method showed that total amount of BSO was released at 110 min.
Conclusion: These methods are considered as a practical method for quantification of BSO for encapsulation efficacy and release. They will help to accelerate and improve routine characterization of encapsulated BSO in food and pharmaceutical technology. |
| first_indexed | 2025-11-14T17:12:20Z |
| format | Article |
| id | iium-66788 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:12:20Z |
| publishDate | 2018 |
| publisher | Innovare Academics Sciences Pvt. Ltd |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-667882018-10-19T07:46:46Z http://irep.iium.edu.my/66788/ ATR-FTIR spectroscopic methods for analysis of black seed oil from alginate beads Alkhatib, Hamzeh Mohamed, Farahidah Doolaanea, Abd Almonem RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry Objective: This study aimed to use attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR) for rapid quantification of black seed oil (BSO) from alginate beads and to develop simple analysis method for in vitro release study based on turbidity measurement. Methods: Guluronic acid-rich (high-G) sodium alginate was used to encapsulate BSO with the aid of Tween 80 as a stabilizer. ATR-FTIR method was developed for quantification of encapsulation efficiency of BSO by applying Beer-Lambert law after selection of a few wave number combinations. UV-vis method based on measurement of emulsion turbidity at 600 nm was also developed to quantify BSO during the releasing from alginate beads in simulated intestine buffer. Results: ATR-FTIR method exhibited linearity in the range of 25-300 mgBSO/mlemulsion (mgBSO/mlE)with R2=0.998, RSD=8.4%, LOD=0.28 mgBSO/mlE and LOQ=0.87 mgBSO/mlE. BSO-alginate beads was found to completely encapsulate BSO with around 100.5% efficiency. UV-vis method exhibited linearity in the range of 50-300 mgBSO/mlE, R2=0.9931, RSD=1.34%, LOD=0.89 mgBSO/mlE, and LOQ=2.71 mgBSO/mlE. In addition, the method showed that total amount of BSO was released at 110 min. Conclusion: These methods are considered as a practical method for quantification of BSO for encapsulation efficacy and release. They will help to accelerate and improve routine characterization of encapsulated BSO in food and pharmaceutical technology. Innovare Academics Sciences Pvt. Ltd 2018-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/66788/1/66788_ATR-FTIR%20spectroscopic%20methods.pdf application/pdf en http://irep.iium.edu.my/66788/2/66788_ATR-FTIR%20spectroscopic%20methods_SCOPUS.pdf Alkhatib, Hamzeh and Mohamed, Farahidah and Doolaanea, Abd Almonem (2018) ATR-FTIR spectroscopic methods for analysis of black seed oil from alginate beads. International Journal of Applied Pharmaceutics, 10 (5 (Sept.-Oct.)). pp. 147-152. ISSN 0975-7058 https://innovareacademics.in/journals/index.php/ijap/article/view/25057/15688 10.22159/ijap.2018v10i5.25057 |
| spellingShingle | RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry Alkhatib, Hamzeh Mohamed, Farahidah Doolaanea, Abd Almonem ATR-FTIR spectroscopic methods for analysis of black seed oil from alginate beads |
| title | ATR-FTIR spectroscopic methods for analysis of black seed oil from alginate beads |
| title_full | ATR-FTIR spectroscopic methods for analysis of black seed oil from alginate beads |
| title_fullStr | ATR-FTIR spectroscopic methods for analysis of black seed oil from alginate beads |
| title_full_unstemmed | ATR-FTIR spectroscopic methods for analysis of black seed oil from alginate beads |
| title_short | ATR-FTIR spectroscopic methods for analysis of black seed oil from alginate beads |
| title_sort | atr-ftir spectroscopic methods for analysis of black seed oil from alginate beads |
| topic | RS Pharmacy and materia medica RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry |
| url | http://irep.iium.edu.my/66788/ http://irep.iium.edu.my/66788/ http://irep.iium.edu.my/66788/ http://irep.iium.edu.my/66788/1/66788_ATR-FTIR%20spectroscopic%20methods.pdf http://irep.iium.edu.my/66788/2/66788_ATR-FTIR%20spectroscopic%20methods_SCOPUS.pdf |