Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells
Tetracera indica Merr.(Family: DILLENIACEAE) is a large, woody, rain forest climber of Malaysia which is commonly known as Mempelas paya or sand paper plant. Two flavonoids MHQ-1 (wogonin) and MHQ-2 (norwogonin) were isolated from the stems ethanol extract of T. indica. Moreover, MHQ-1 and MHQ-2...
| Main Authors: | , , , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/65520/ http://irep.iium.edu.my/65520/1/rcstss2016_abstracts_ST_final_secured.pdf |
| _version_ | 1848786404162666496 |
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| author | Ahmed, Qamar Uddin Hasan, Mohd Mehmudul Sarian, Murni Nazira Mat So'ad, Siti Zaiton Bakhtiar, M. Taher Latip, Jalifah |
| author_facet | Ahmed, Qamar Uddin Hasan, Mohd Mehmudul Sarian, Murni Nazira Mat So'ad, Siti Zaiton Bakhtiar, M. Taher Latip, Jalifah |
| author_sort | Ahmed, Qamar Uddin |
| building | IIUM Repository |
| collection | Online Access |
| description | Tetracera indica Merr.(Family: DILLENIACEAE) is a large, woody, rain
forest climber of Malaysia which is commonly known as Mempelas paya or sand
paper plant. Two flavonoids MHQ-1 (wogonin) and MHQ-2 (norwogonin) were
isolated from the stems ethanol extract of T. indica. Moreover, MHQ-1 and MHQ-2
including rosiglitazone as positive control were subjected to fluorescence glucose
uptake test by 2-NBDG (fluorescent glucose analog) on mature adipocytes. Wogonin
was found to show more cytotoxicity (IC50 <25 µg/mL) in comparison to
norwogonin (no IC50 upto100 µg/mL) in MTT viability assay on 3T3-L1 preadipocyte
cells. Structure activity relationships (SAR) study of both the flavonoids
revealed that the methoxy (O-Me) substitution (wogonin) at position 8 of ring A of
flavone skeleton is essential for inducing cytotoxicity on 3T3-L1 Pre-adipocytes.
However, hydroxyl (OH) group at the same position (norwogonin) is safe to the cells
(percent viability is above 80% for all the doses evaluated). Additionally, both the
compounds were introduced to the cells in different safe concentrations as well as in
different adipogenic cocktails. The adipogenic cocktails were modified by the
compounds and rosiglitazone in the presence or absence of insulin. The results of
adipogenesis showed that MHQ-1 and MHQ-2 induced adipogenesis like insulin and
enhanced adipogenesis like rosiglitazone significantly in three different
concentrations. Furthermore, both the compound stimulated the 2-NBDG uptake on
mature adipocytes significantly. Results further confirm the traditional use of T.
indica in the management of diabetes in Malaysia. |
| first_indexed | 2025-11-14T17:08:30Z |
| format | Proceeding Paper |
| id | iium-65520 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T17:08:30Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-655202018-09-18T04:30:10Z http://irep.iium.edu.my/65520/ Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells Ahmed, Qamar Uddin Hasan, Mohd Mehmudul Sarian, Murni Nazira Mat So'ad, Siti Zaiton Bakhtiar, M. Taher Latip, Jalifah RS403 Materia Medica-Pharmaceutical Chemistry Tetracera indica Merr.(Family: DILLENIACEAE) is a large, woody, rain forest climber of Malaysia which is commonly known as Mempelas paya or sand paper plant. Two flavonoids MHQ-1 (wogonin) and MHQ-2 (norwogonin) were isolated from the stems ethanol extract of T. indica. Moreover, MHQ-1 and MHQ-2 including rosiglitazone as positive control were subjected to fluorescence glucose uptake test by 2-NBDG (fluorescent glucose analog) on mature adipocytes. Wogonin was found to show more cytotoxicity (IC50 <25 µg/mL) in comparison to norwogonin (no IC50 upto100 µg/mL) in MTT viability assay on 3T3-L1 preadipocyte cells. Structure activity relationships (SAR) study of both the flavonoids revealed that the methoxy (O-Me) substitution (wogonin) at position 8 of ring A of flavone skeleton is essential for inducing cytotoxicity on 3T3-L1 Pre-adipocytes. However, hydroxyl (OH) group at the same position (norwogonin) is safe to the cells (percent viability is above 80% for all the doses evaluated). Additionally, both the compounds were introduced to the cells in different safe concentrations as well as in different adipogenic cocktails. The adipogenic cocktails were modified by the compounds and rosiglitazone in the presence or absence of insulin. The results of adipogenesis showed that MHQ-1 and MHQ-2 induced adipogenesis like insulin and enhanced adipogenesis like rosiglitazone significantly in three different concentrations. Furthermore, both the compound stimulated the 2-NBDG uptake on mature adipocytes significantly. Results further confirm the traditional use of T. indica in the management of diabetes in Malaysia. 2016 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/65520/1/rcstss2016_abstracts_ST_final_secured.pdf Ahmed, Qamar Uddin and Hasan, Mohd Mehmudul and Sarian, Murni Nazira and Mat So'ad, Siti Zaiton and Bakhtiar, M. Taher and Latip, Jalifah (2016) Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells. In: Regional Conference On Science, Technology & Social Sciences 2016 (RCSTSS), 4th - 6th December 2016, Cameron Highlands, Pahang. (Unpublished) |
| spellingShingle | RS403 Materia Medica-Pharmaceutical Chemistry Ahmed, Qamar Uddin Hasan, Mohd Mehmudul Sarian, Murni Nazira Mat So'ad, Siti Zaiton Bakhtiar, M. Taher Latip, Jalifah Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells |
| title | Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells |
| title_full | Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells |
| title_fullStr | Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells |
| title_full_unstemmed | Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells |
| title_short | Flavonoids from Tetracera indica Merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3T3-L1 adipocyte cells |
| title_sort | flavonoids from tetracera indica merr. inhibit adipocyte differentiation and exert glucose uptake activities in 3t3-l1 adipocyte cells |
| topic | RS403 Materia Medica-Pharmaceutical Chemistry |
| url | http://irep.iium.edu.my/65520/ http://irep.iium.edu.my/65520/1/rcstss2016_abstracts_ST_final_secured.pdf |