Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species
In this paper, the potency of stem and leave extracts of Leea indica locally known as Memali from Tasik Chini, Pahang as a potent antioxidant agent had been studied. The antioxidant properties of three types of extracts was quantitatively assessed by 2,2 diphenyl-1-picrylhydrazyl ( DPPH) radical...
| Main Authors: | , , , , , , , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/65519/ http://irep.iium.edu.my/65519/1/rcstss2016_abstracts_ST_final_secured.pdf |
| _version_ | 1848786403871162368 |
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| author | Harun, Aiza Daud, Shaari Harith, Siti Suhaila Ab Rahim, Nor Erlena Asmira Abd Jalil, Mohd Azfar Muhammad Rosdi, Nurul ‘Atiqah , Fatin Azieyati , Naila Nabihah Mat So'ad, Siti Zaiton Mohd Hassan, Norazian |
| author_facet | Harun, Aiza Daud, Shaari Harith, Siti Suhaila Ab Rahim, Nor Erlena Asmira Abd Jalil, Mohd Azfar Muhammad Rosdi, Nurul ‘Atiqah , Fatin Azieyati , Naila Nabihah Mat So'ad, Siti Zaiton Mohd Hassan, Norazian |
| author_sort | Harun, Aiza |
| building | IIUM Repository |
| collection | Online Access |
| description | In this paper, the potency of stem and leave extracts of Leea indica locally
known as Memali from Tasik Chini, Pahang as a potent antioxidant agent had been
studied. The antioxidant properties of three types of extracts was quantitatively
assessed by 2,2 diphenyl-1-picrylhydrazyl ( DPPH) radical scavenging activity. The
isolation of antioxidative compounds was conducted through preparative thin layer
chromatography with the use of certain binary solvents. The structure determination
was carried out using Gas Chromatography Mass Spectrometer (GCMS). The
methanol extracts from both stem and leaves exhibited highest antioxidant activity
with lowest IC50 of 24 ug/ml and 81 ug/ml respectively compared to other extracts.
The stem showed stronger antioxidant activity compared to leaves part. Based on
GCMS analysis, the antioxidative compounds isolated from leaves part were
proposed as 6-Hyroxy-4,4, 7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one
(1), 3,8,8-Trimethoxy-3-piperidyl-2,2-binaphthalene-1,1,4,4-tetrone (2), Methyl
stearate (3), 9,12-Octadecadienoic acid (4), Palmitic acid (5) and 9-Oxononanoic
acid (6). The antioxidative compounds isolated from stem part were determined as 1-
(hydroxymethyl)-1,2-ethanediyl ester (7), 9-octadecene (8) and oleic acid (9). This
finding can be used as scientific baseline information for the future treatment of
diseases caused by harmful free radicals. |
| first_indexed | 2025-11-14T17:08:29Z |
| format | Proceeding Paper |
| id | iium-65519 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T17:08:29Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-655192018-09-18T06:28:11Z http://irep.iium.edu.my/65519/ Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species Harun, Aiza Daud, Shaari Harith, Siti Suhaila Ab Rahim, Nor Erlena Asmira Abd Jalil, Mohd Azfar Muhammad Rosdi, Nurul ‘Atiqah , Fatin Azieyati , Naila Nabihah Mat So'ad, Siti Zaiton Mohd Hassan, Norazian QD Chemistry In this paper, the potency of stem and leave extracts of Leea indica locally known as Memali from Tasik Chini, Pahang as a potent antioxidant agent had been studied. The antioxidant properties of three types of extracts was quantitatively assessed by 2,2 diphenyl-1-picrylhydrazyl ( DPPH) radical scavenging activity. The isolation of antioxidative compounds was conducted through preparative thin layer chromatography with the use of certain binary solvents. The structure determination was carried out using Gas Chromatography Mass Spectrometer (GCMS). The methanol extracts from both stem and leaves exhibited highest antioxidant activity with lowest IC50 of 24 ug/ml and 81 ug/ml respectively compared to other extracts. The stem showed stronger antioxidant activity compared to leaves part. Based on GCMS analysis, the antioxidative compounds isolated from leaves part were proposed as 6-Hyroxy-4,4, 7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)-one (1), 3,8,8-Trimethoxy-3-piperidyl-2,2-binaphthalene-1,1,4,4-tetrone (2), Methyl stearate (3), 9,12-Octadecadienoic acid (4), Palmitic acid (5) and 9-Oxononanoic acid (6). The antioxidative compounds isolated from stem part were determined as 1- (hydroxymethyl)-1,2-ethanediyl ester (7), 9-octadecene (8) and oleic acid (9). This finding can be used as scientific baseline information for the future treatment of diseases caused by harmful free radicals. 2016 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/65519/1/rcstss2016_abstracts_ST_final_secured.pdf Harun, Aiza and Daud, Shaari and Harith, Siti Suhaila and Ab Rahim, Nor Erlena Asmira and Abd Jalil, Mohd Azfar and Muhammad Rosdi, Nurul ‘Atiqah and , Fatin Azieyati and , Naila Nabihah and Mat So'ad, Siti Zaiton and Mohd Hassan, Norazian (2016) Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species. In: Regional Conference On Science, Technology & Social Sciences 2016 (RCSTSS), 4th - 6th December 2016, Cameron Highlands, Pahang. (Unpublished) |
| spellingShingle | QD Chemistry Harun, Aiza Daud, Shaari Harith, Siti Suhaila Ab Rahim, Nor Erlena Asmira Abd Jalil, Mohd Azfar Muhammad Rosdi, Nurul ‘Atiqah , Fatin Azieyati , Naila Nabihah Mat So'ad, Siti Zaiton Mohd Hassan, Norazian Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species |
| title | Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species |
| title_full | Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species |
| title_fullStr | Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species |
| title_full_unstemmed | Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species |
| title_short | Radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of Leea indica species |
| title_sort | radical scavenging activity and phytochemical investigation of antioxidative compounds from stem and leaves of leea indica species |
| topic | QD Chemistry |
| url | http://irep.iium.edu.my/65519/ http://irep.iium.edu.my/65519/1/rcstss2016_abstracts_ST_final_secured.pdf |