Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms
Introduction: Inherent resistance of biofilm bacteria to conventional antibiotics is alarming because biofilms induce antibiotic resistance to an order of three or more in magnitude greater than those displayed by planktonic bacteria. Pseudomonas aeruginosa is an infectious organism that causes t...
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| Format: | Article |
| Language: | English |
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Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia
2016
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| Online Access: | http://irep.iium.edu.my/65153/ http://irep.iium.edu.my/65153/1/The%20International%20Medical%20Journal%20Malaysia%20%282016%29%20Vol%2015%20Supplementary%20issue%20No1.pdf |
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| author | Shahdan, Intan Azura Mohd Sobri, Fatimah Zahrah Hanapi, Mohammad Faiz Hizzuan Ahmad Yusof @ Hanafi, Hanani How, Fiona Ni Foong |
| author_facet | Shahdan, Intan Azura Mohd Sobri, Fatimah Zahrah Hanapi, Mohammad Faiz Hizzuan Ahmad Yusof @ Hanafi, Hanani How, Fiona Ni Foong |
| author_sort | Shahdan, Intan Azura |
| building | IIUM Repository |
| collection | Online Access |
| description | Introduction: Inherent resistance of biofilm bacteria to conventional antibiotics is alarming
because biofilms induce antibiotic resistance to an order of three or more in magnitude
greater than those displayed by planktonic bacteria. Pseudomonas aeruginosa is an infectious
organism that causes the hallmark of chronic infections including hospital acquired infections
that leads to high morbidity and mortality. One of the reasons for the occurrence of
resistance is its ability to form biofilms. In this study, the resistance of P. aeruginosa biofilms
against a series of metal-antibiotics, an alternative to the conventional antibiotics, was
investigated. Methods: A series of metal-antibiotic complexes derived from gentamicin was
synthesized to give metal-gentamicin complexes. The metal contents of all the compounds
were determined using Atomic Absorption Spectroscopy (AAS). Antibiotic susceptibility testing
of the gentamicin-antibiotic complexes against the biofilms was conducted using broth
microdilution assay. Results: The results showed that P. aeruginosa is susceptible against all
the metal-gentamicin complexes, which include, Ni(II), Fe(II), Cu(II), Zn(II) and Co(II)
complexes; all were tested at 0.25 to 1 mmol concentrations. Conclusions: The results show
that all metal-gentamicin complexes have higher antimicrobial activity than gentamicin (0.25
to 1 mmol) on its own. Finally, mechanisms of P. aeruginosa biofilms resistance to these
metal-antibiotics are also proposed. |
| first_indexed | 2025-11-14T17:07:28Z |
| format | Article |
| id | iium-65153 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T17:07:28Z |
| publishDate | 2016 |
| publisher | Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-651532018-08-17T01:19:19Z http://irep.iium.edu.my/65153/ Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms Shahdan, Intan Azura Mohd Sobri, Fatimah Zahrah Hanapi, Mohammad Faiz Hizzuan Ahmad Yusof @ Hanafi, Hanani How, Fiona Ni Foong QD Chemistry Introduction: Inherent resistance of biofilm bacteria to conventional antibiotics is alarming because biofilms induce antibiotic resistance to an order of three or more in magnitude greater than those displayed by planktonic bacteria. Pseudomonas aeruginosa is an infectious organism that causes the hallmark of chronic infections including hospital acquired infections that leads to high morbidity and mortality. One of the reasons for the occurrence of resistance is its ability to form biofilms. In this study, the resistance of P. aeruginosa biofilms against a series of metal-antibiotics, an alternative to the conventional antibiotics, was investigated. Methods: A series of metal-antibiotic complexes derived from gentamicin was synthesized to give metal-gentamicin complexes. The metal contents of all the compounds were determined using Atomic Absorption Spectroscopy (AAS). Antibiotic susceptibility testing of the gentamicin-antibiotic complexes against the biofilms was conducted using broth microdilution assay. Results: The results showed that P. aeruginosa is susceptible against all the metal-gentamicin complexes, which include, Ni(II), Fe(II), Cu(II), Zn(II) and Co(II) complexes; all were tested at 0.25 to 1 mmol concentrations. Conclusions: The results show that all metal-gentamicin complexes have higher antimicrobial activity than gentamicin (0.25 to 1 mmol) on its own. Finally, mechanisms of P. aeruginosa biofilms resistance to these metal-antibiotics are also proposed. Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2016-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/65153/1/The%20International%20Medical%20Journal%20Malaysia%20%282016%29%20Vol%2015%20Supplementary%20issue%20No1.pdf Shahdan, Intan Azura and Mohd Sobri, Fatimah Zahrah and Hanapi, Mohammad Faiz Hizzuan and Ahmad Yusof @ Hanafi, Hanani and How, Fiona Ni Foong (2016) Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms. International Medical Journal Malaysia, 15 (Supplement Issue). p. 9. E-ISSN 1823-4631 http://iiumedic.net/imjm/v1/download/Volume%2015%20Supplement/MRS2016-ABSTRACT-BOOK-9.pdf |
| spellingShingle | QD Chemistry Shahdan, Intan Azura Mohd Sobri, Fatimah Zahrah Hanapi, Mohammad Faiz Hizzuan Ahmad Yusof @ Hanafi, Hanani How, Fiona Ni Foong Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms |
| title | Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms |
| title_full | Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms |
| title_fullStr | Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms |
| title_full_unstemmed | Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms |
| title_short | Antibiotic susceptibility study of metal-gentamicin complexes against Pseudomonas aeruginosa biofilms |
| title_sort | antibiotic susceptibility study of metal-gentamicin complexes against pseudomonas aeruginosa biofilms |
| topic | QD Chemistry |
| url | http://irep.iium.edu.my/65153/ http://irep.iium.edu.my/65153/ http://irep.iium.edu.my/65153/1/The%20International%20Medical%20Journal%20Malaysia%20%282016%29%20Vol%2015%20Supplementary%20issue%20No1.pdf |