A simple method for extracting both active oily and water soluble extract (WSE) from Nigella sativa (L.) seeds using a single solvent system
The current study provides a way of extraction for both active NSO and WSE from Nigella sativa seeds using 98% methanol. About 1 kg of ground seeds was macerated by 1:2.5 w/v (g/mL) for 72 hours. After rotary evaporation and 7 days of continuous drying and chilling at 50 and 4 °C, NSO and WSE were o...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English English English |
| Published: |
Taylor and Francis
2018
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/65108/ http://irep.iium.edu.my/65108/16/65108-A%20simple%20method%20for%20extracting%20both%20active%20oily%20-article%20for%20myra.pdf http://irep.iium.edu.my/65108/9/65108%20A%20simple%20method%20for%20extracting%20both%20active%20oily%20and%20water%20soluble%20extract%20SCOPUS-in-press.pdf http://irep.iium.edu.my/65108/17/65108_A%20simple%20method%20for%20extracting%20both%20active%20oily_wos.pdf |
| _version_ | 1848786330840989696 |
|---|---|
| author | Babar, Z.M. Wan Sulaiman, Wan Mohd. Azizi Arief Ichwan, Solachuddin Jauhari Ahmed, Qamar Uddin Azad, Abul Kalam Mawa, Imranul |
| author_facet | Babar, Z.M. Wan Sulaiman, Wan Mohd. Azizi Arief Ichwan, Solachuddin Jauhari Ahmed, Qamar Uddin Azad, Abul Kalam Mawa, Imranul |
| author_sort | Babar, Z.M. |
| building | IIUM Repository |
| collection | Online Access |
| description | The current study provides a way of extraction for both active NSO and WSE from Nigella sativa seeds using 98% methanol. About 1 kg of ground seeds was macerated by 1:2.5 w/v (g/mL) for 72 hours. After rotary evaporation and 7 days of continuous drying and chilling at 50 and 4 °C, NSO and WSE were obtained at the same instant. Solubility tests of 24 solvents and 11 thin layer chromatographic analyses while 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging assay of NSO (73.66) , WSE (33.32) and NSO + WSE (78.22) against ascorbic acid (IC50 = 4.28 mg/mL) was performed. WSE was found to be highly soluble in water and 5% NaOH exhibiting the same Rf value of 0.95 for EtOH:DMSO (9:1) against the honey. WSE has revealed more than twofold higher anti-oxidant activity than others. Formulation of WSE with Tualang honey may provide better targeted hydrophilic drug delivery systems. |
| first_indexed | 2025-11-14T17:07:20Z |
| format | Article |
| id | iium-65108 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:07:20Z |
| publishDate | 2018 |
| publisher | Taylor and Francis |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-651082020-04-09T14:23:11Z http://irep.iium.edu.my/65108/ A simple method for extracting both active oily and water soluble extract (WSE) from Nigella sativa (L.) seeds using a single solvent system Babar, Z.M. Wan Sulaiman, Wan Mohd. Azizi Arief Ichwan, Solachuddin Jauhari Ahmed, Qamar Uddin Azad, Abul Kalam Mawa, Imranul Q Science (General) The current study provides a way of extraction for both active NSO and WSE from Nigella sativa seeds using 98% methanol. About 1 kg of ground seeds was macerated by 1:2.5 w/v (g/mL) for 72 hours. After rotary evaporation and 7 days of continuous drying and chilling at 50 and 4 °C, NSO and WSE were obtained at the same instant. Solubility tests of 24 solvents and 11 thin layer chromatographic analyses while 2, 2-diphenyl-1-picrylhydrazyl free radical scavenging assay of NSO (73.66) , WSE (33.32) and NSO + WSE (78.22) against ascorbic acid (IC50 = 4.28 mg/mL) was performed. WSE was found to be highly soluble in water and 5% NaOH exhibiting the same Rf value of 0.95 for EtOH:DMSO (9:1) against the honey. WSE has revealed more than twofold higher anti-oxidant activity than others. Formulation of WSE with Tualang honey may provide better targeted hydrophilic drug delivery systems. Taylor and Francis 2018-07-24 Article PeerReviewed application/pdf en http://irep.iium.edu.my/65108/16/65108-A%20simple%20method%20for%20extracting%20both%20active%20oily%20-article%20for%20myra.pdf application/pdf en http://irep.iium.edu.my/65108/9/65108%20A%20simple%20method%20for%20extracting%20both%20active%20oily%20and%20water%20soluble%20extract%20SCOPUS-in-press.pdf application/pdf en http://irep.iium.edu.my/65108/17/65108_A%20simple%20method%20for%20extracting%20both%20active%20oily_wos.pdf Babar, Z.M. and Wan Sulaiman, Wan Mohd. Azizi and Arief Ichwan, Solachuddin Jauhari and Ahmed, Qamar Uddin and Azad, Abul Kalam and Mawa, Imranul (2018) A simple method for extracting both active oily and water soluble extract (WSE) from Nigella sativa (L.) seeds using a single solvent system. Natural Product Research. pp. 1-6. ISSN 1478-6419 E-ISSN 1478-6427 (In Press) https://www.tandfonline.com/doi/abs/10.1080/14786419.2018.1493587 10.1080/14786419.2018.1493587587 |
| spellingShingle | Q Science (General) Babar, Z.M. Wan Sulaiman, Wan Mohd. Azizi Arief Ichwan, Solachuddin Jauhari Ahmed, Qamar Uddin Azad, Abul Kalam Mawa, Imranul A simple method for extracting both active oily and water soluble extract (WSE) from Nigella sativa (L.) seeds using a single solvent system |
| title | A simple method for extracting both active oily and water soluble extract (WSE) from Nigella sativa (L.) seeds using a single solvent system |
| title_full | A simple method for extracting both active oily and water soluble extract (WSE) from Nigella sativa (L.) seeds using a single solvent system |
| title_fullStr | A simple method for extracting both active oily and water soluble extract (WSE) from Nigella sativa (L.) seeds using a single solvent system |
| title_full_unstemmed | A simple method for extracting both active oily and water soluble extract (WSE) from Nigella sativa (L.) seeds using a single solvent system |
| title_short | A simple method for extracting both active oily and water soluble extract (WSE) from Nigella sativa (L.) seeds using a single solvent system |
| title_sort | simple method for extracting both active oily and water soluble extract (wse) from nigella sativa (l.) seeds using a single solvent system |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/65108/ http://irep.iium.edu.my/65108/ http://irep.iium.edu.my/65108/ http://irep.iium.edu.my/65108/16/65108-A%20simple%20method%20for%20extracting%20both%20active%20oily%20-article%20for%20myra.pdf http://irep.iium.edu.my/65108/9/65108%20A%20simple%20method%20for%20extracting%20both%20active%20oily%20and%20water%20soluble%20extract%20SCOPUS-in-press.pdf http://irep.iium.edu.my/65108/17/65108_A%20simple%20method%20for%20extracting%20both%20active%20oily_wos.pdf |