Quantitative protein expression of Malaysian Phaleriamacrocarpa

Herbal plants are the best alternative for synthetic medicines which are costly and proved to be double-edged source. P. macrocarpais the traditional herb compatible in curing diseases like diabetes, inflammation, and even cancer. People have gained lots of benefits...

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Main Authors: Muhamad Bunnori, Noraslinda, Hamdan, Nurul Ashima, Abu Hassan, Muhamad Shafiq, Mohamed Rehan, Aisyah, Abd Halim, Khairul Bariyyah, Haron, Normah
Format: Article
Language:English
Published: Institute of Research and Journals 2018
Subjects:
Online Access:http://irep.iium.edu.my/64629/
http://irep.iium.edu.my/64629/1/IJASEAT_Noraslinda.pdf
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author Muhamad Bunnori, Noraslinda
Hamdan, Nurul Ashima
Abu Hassan, Muhamad Shafiq
Mohamed Rehan, Aisyah
Abd Halim, Khairul Bariyyah
Haron, Normah
author_facet Muhamad Bunnori, Noraslinda
Hamdan, Nurul Ashima
Abu Hassan, Muhamad Shafiq
Mohamed Rehan, Aisyah
Abd Halim, Khairul Bariyyah
Haron, Normah
author_sort Muhamad Bunnori, Noraslinda
building IIUM Repository
collection Online Access
description Herbal plants are the best alternative for synthetic medicines which are costly and proved to be double-edged source. P. macrocarpais the traditional herb compatible in curing diseases like diabetes, inflammation, and even cancer. People have gained lots of benefits from most parts of the plant with largest usage of seeds. This study aimed to analyze protein expressions in three major parts of the plant; leaf, seed and fruit by using SDS PAGE and high-throughput two-dimensional gel electrophoresis as proteomics approached. There were 42 spots detected in a pH range of 3-10 with 3 spots have been analyzed by MALDI-TOF mass spectrometry. Most identified proteins were expressed in seeds with highest intensity of bands and spots. The identified spots are homologs to putative clathrin assembly protein and ferredoxin NADP+ reductase which are characterized as ‘housekeeping agent’ in plant system.
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publishDate 2018
publisher Institute of Research and Journals
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spelling iium-646292018-07-11T08:00:30Z http://irep.iium.edu.my/64629/ Quantitative protein expression of Malaysian Phaleriamacrocarpa Muhamad Bunnori, Noraslinda Hamdan, Nurul Ashima Abu Hassan, Muhamad Shafiq Mohamed Rehan, Aisyah Abd Halim, Khairul Bariyyah Haron, Normah QK Botany Herbal plants are the best alternative for synthetic medicines which are costly and proved to be double-edged source. P. macrocarpais the traditional herb compatible in curing diseases like diabetes, inflammation, and even cancer. People have gained lots of benefits from most parts of the plant with largest usage of seeds. This study aimed to analyze protein expressions in three major parts of the plant; leaf, seed and fruit by using SDS PAGE and high-throughput two-dimensional gel electrophoresis as proteomics approached. There were 42 spots detected in a pH range of 3-10 with 3 spots have been analyzed by MALDI-TOF mass spectrometry. Most identified proteins were expressed in seeds with highest intensity of bands and spots. The identified spots are homologs to putative clathrin assembly protein and ferredoxin NADP+ reductase which are characterized as ‘housekeeping agent’ in plant system. Institute of Research and Journals 2018-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/64629/1/IJASEAT_Noraslinda.pdf Muhamad Bunnori, Noraslinda and Hamdan, Nurul Ashima and Abu Hassan, Muhamad Shafiq and Mohamed Rehan, Aisyah and Abd Halim, Khairul Bariyyah and Haron, Normah (2018) Quantitative protein expression of Malaysian Phaleriamacrocarpa. International Journal of Advances in Science Engineering and Technology , I, 6 (2). pp. 18-22. ISSN 2321-8991 E-ISSN 2321-9009 http://www.iraj.in/journal/journal_file/journal_pdf/6-462-153070557818-22.pdf IJASEAT-IRAJ-DOI-11995
spellingShingle QK Botany
Muhamad Bunnori, Noraslinda
Hamdan, Nurul Ashima
Abu Hassan, Muhamad Shafiq
Mohamed Rehan, Aisyah
Abd Halim, Khairul Bariyyah
Haron, Normah
Quantitative protein expression of Malaysian Phaleriamacrocarpa
title Quantitative protein expression of Malaysian Phaleriamacrocarpa
title_full Quantitative protein expression of Malaysian Phaleriamacrocarpa
title_fullStr Quantitative protein expression of Malaysian Phaleriamacrocarpa
title_full_unstemmed Quantitative protein expression of Malaysian Phaleriamacrocarpa
title_short Quantitative protein expression of Malaysian Phaleriamacrocarpa
title_sort quantitative protein expression of malaysian phaleriamacrocarpa
topic QK Botany
url http://irep.iium.edu.my/64629/
http://irep.iium.edu.my/64629/
http://irep.iium.edu.my/64629/
http://irep.iium.edu.my/64629/1/IJASEAT_Noraslinda.pdf