Acute and sub-acute antihypertensive effects of syzygium polyanthum leaf extracts with determination of gallic a using HPLC analysis
Context: Aqueous decoction of Syzygium polyanthum (ADSP) leaf is one of Malay traditionalremedial- preparations for hypertension. Intravenous ADSP reduced blood pressure of anesthetized Wistar-Kyoto (WKY) and Spontaneously-Hypertensive rats (SHR); however, acute and sub-acute effects of oral ADSP...
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Phcog.net
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| Online Access: | http://irep.iium.edu.my/64565/ http://irep.iium.edu.my/64565/1/PharmacognJ-10-663%20%284%29.pdf http://irep.iium.edu.my/64565/7/64565_Acute%20and%20sub-acute%20antihypertensive%20effects%20of%20syzygium_scopus.pdf |
| _version_ | 1848786239946227712 |
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| author | Ismail, Azlini Ramli, Nurul Syahida Mohamed, Mahaneem Wan Ahmad, Wan Amir Nizam |
| author_facet | Ismail, Azlini Ramli, Nurul Syahida Mohamed, Mahaneem Wan Ahmad, Wan Amir Nizam |
| author_sort | Ismail, Azlini |
| building | IIUM Repository |
| collection | Online Access |
| description | Context: Aqueous decoction of Syzygium polyanthum (ADSP) leaf is one of Malay traditionalremedial-
preparations for hypertension. Intravenous ADSP reduced blood pressure of anesthetized
Wistar-Kyoto (WKY) and Spontaneously-Hypertensive rats (SHR); however, acute
and sub-acute effects of oral ADSP on conscious rats and its bioactive compound(s) are not
comprehensively studied. This study aims to examine the acute and sub-acute effects of
ADSP in comparison to methanol extract (MESP) on systolic blood pressure (SBP) of conscious
WKY and SHR rats, as well as to identify their major phenolic-compound using highperformance-
liquid-chromatography (HPLC). Methodology: For acute study, SHR and WKY
rats were gavaged with single-dose of ADSP or MESP (2.00, 2.50 or 3.00 g/kg), vehicles, or
losartan (0.01 g/kg). SBP was measured after 1, 3, 5, 6 and 24 hr-post-administration. For subacute
experiment, SHR rats were gavaged once-daily with ADSP or MESP (2.50 g/kg/day),
vehicles, or losartan (0.01 g/kg/day) for 3-week and their SBP was weekly-measured. Phenolic
compounds were screened using ferric-chloride test, then gallic acid was determined using
HPLC. Results: Acute administration of ADSP (2.50 to 3.00 g/kg) and MESP (2.00 to 3.00 g/
kg) significantly reduced SBP of SHR, but not of WKY rats. Repeated-daily-dose administration
of MESP (2.5 g/kg/day) significantly reduced SBP of SHR after 2-week and sustained until
3-week, while ADSP (2.5 g/kg/day) significantly reduced SBP only after 3-week. Gallic acid
was the major phenolic-compound in ADSP (157.09 ± 4.18 ppm) and MESP (134.04 ± 10.30
ppm). Conclusion: Oral ADSP and MESP that contain gallic acid possess acute and sub-acute
antihypertensive effects in SHR, but MESP’s effect was more prominent than ADSP. |
| first_indexed | 2025-11-14T17:05:53Z |
| format | Article |
| id | iium-64565 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:05:53Z |
| publishDate | 2018 |
| publisher | Phcog.net |
| recordtype | eprints |
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| spelling | iium-645652018-10-16T01:07:21Z http://irep.iium.edu.my/64565/ Acute and sub-acute antihypertensive effects of syzygium polyanthum leaf extracts with determination of gallic a using HPLC analysis Ismail, Azlini Ramli, Nurul Syahida Mohamed, Mahaneem Wan Ahmad, Wan Amir Nizam RM Therapeutics. Pharmacology RS Pharmacy and materia medica Context: Aqueous decoction of Syzygium polyanthum (ADSP) leaf is one of Malay traditionalremedial- preparations for hypertension. Intravenous ADSP reduced blood pressure of anesthetized Wistar-Kyoto (WKY) and Spontaneously-Hypertensive rats (SHR); however, acute and sub-acute effects of oral ADSP on conscious rats and its bioactive compound(s) are not comprehensively studied. This study aims to examine the acute and sub-acute effects of ADSP in comparison to methanol extract (MESP) on systolic blood pressure (SBP) of conscious WKY and SHR rats, as well as to identify their major phenolic-compound using highperformance- liquid-chromatography (HPLC). Methodology: For acute study, SHR and WKY rats were gavaged with single-dose of ADSP or MESP (2.00, 2.50 or 3.00 g/kg), vehicles, or losartan (0.01 g/kg). SBP was measured after 1, 3, 5, 6 and 24 hr-post-administration. For subacute experiment, SHR rats were gavaged once-daily with ADSP or MESP (2.50 g/kg/day), vehicles, or losartan (0.01 g/kg/day) for 3-week and their SBP was weekly-measured. Phenolic compounds were screened using ferric-chloride test, then gallic acid was determined using HPLC. Results: Acute administration of ADSP (2.50 to 3.00 g/kg) and MESP (2.00 to 3.00 g/ kg) significantly reduced SBP of SHR, but not of WKY rats. Repeated-daily-dose administration of MESP (2.5 g/kg/day) significantly reduced SBP of SHR after 2-week and sustained until 3-week, while ADSP (2.5 g/kg/day) significantly reduced SBP only after 3-week. Gallic acid was the major phenolic-compound in ADSP (157.09 ± 4.18 ppm) and MESP (134.04 ± 10.30 ppm). Conclusion: Oral ADSP and MESP that contain gallic acid possess acute and sub-acute antihypertensive effects in SHR, but MESP’s effect was more prominent than ADSP. Phcog.net 2018-07 Article PeerReviewed application/pdf en http://irep.iium.edu.my/64565/1/PharmacognJ-10-663%20%284%29.pdf application/pdf en http://irep.iium.edu.my/64565/7/64565_Acute%20and%20sub-acute%20antihypertensive%20effects%20of%20syzygium_scopus.pdf Ismail, Azlini and Ramli, Nurul Syahida and Mohamed, Mahaneem and Wan Ahmad, Wan Amir Nizam (2018) Acute and sub-acute antihypertensive effects of syzygium polyanthum leaf extracts with determination of gallic a using HPLC analysis. Pharmacognosy Journal, 10 (4 (July-August)). pp. 663-671. ISSN 0975-3575 http://www.phcogj.com/sites/default/files/PharmacognJ-10-663_0.pdf 10.5530/pj.2018.4.109 |
| spellingShingle | RM Therapeutics. Pharmacology RS Pharmacy and materia medica Ismail, Azlini Ramli, Nurul Syahida Mohamed, Mahaneem Wan Ahmad, Wan Amir Nizam Acute and sub-acute antihypertensive effects of syzygium polyanthum leaf extracts with determination of gallic a using HPLC analysis |
| title | Acute and sub-acute antihypertensive effects of syzygium
polyanthum leaf extracts with determination of gallic a
using HPLC analysis |
| title_full | Acute and sub-acute antihypertensive effects of syzygium
polyanthum leaf extracts with determination of gallic a
using HPLC analysis |
| title_fullStr | Acute and sub-acute antihypertensive effects of syzygium
polyanthum leaf extracts with determination of gallic a
using HPLC analysis |
| title_full_unstemmed | Acute and sub-acute antihypertensive effects of syzygium
polyanthum leaf extracts with determination of gallic a
using HPLC analysis |
| title_short | Acute and sub-acute antihypertensive effects of syzygium
polyanthum leaf extracts with determination of gallic a
using HPLC analysis |
| title_sort | acute and sub-acute antihypertensive effects of syzygium
polyanthum leaf extracts with determination of gallic a
using hplc analysis |
| topic | RM Therapeutics. Pharmacology RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/64565/ http://irep.iium.edu.my/64565/ http://irep.iium.edu.my/64565/ http://irep.iium.edu.my/64565/1/PharmacognJ-10-663%20%284%29.pdf http://irep.iium.edu.my/64565/7/64565_Acute%20and%20sub-acute%20antihypertensive%20effects%20of%20syzygium_scopus.pdf |