Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin

Background This study aimed to fabricate a denture base resin (DBR) containing phytoncide microcapsules (PTMCs) and determine the mechanical properties of the resin and antifungal activity. Methods Fifty‐four heat cured rectangular DBR specimens (64 x 10 x 3.3 ± 0.2 mm) containing nine concentr...

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Main Authors: Steve, An, Judge, Roy B, Wong, Rebecca H, Arzmi, Mohd Hafiz, Palamara, J E, Dashper, Stuart
Format: Article
Language:English
English
English
Published: Wiley Online Library 2018
Subjects:
Online Access:http://irep.iium.edu.my/64442/
http://irep.iium.edu.my/64442/7/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial_SCOPUS.pdf
http://irep.iium.edu.my/64442/19/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial.pdf
http://irep.iium.edu.my/64442/20/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial_WOS.pdf
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author Steve, An
Judge, Roy B
Wong, Rebecca H
Arzmi, Mohd Hafiz
Palamara, J E
Dashper, Stuart
author_facet Steve, An
Judge, Roy B
Wong, Rebecca H
Arzmi, Mohd Hafiz
Palamara, J E
Dashper, Stuart
author_sort Steve, An
building IIUM Repository
collection Online Access
description Background This study aimed to fabricate a denture base resin (DBR) containing phytoncide microcapsules (PTMCs) and determine the mechanical properties of the resin and antifungal activity. Methods Fifty‐four heat cured rectangular DBR specimens (64 x 10 x 3.3 ± 0.2 mm) containing nine concentrations of PTMC between 0 ‐ 5% (wt/wt) were fabricated and subjected to a three‐point bending test. A phytoncide release bioassay was developed using DBR containing 0% and 2.5% PTMCs (wt/wt) in a 24 well‐plate assay with incubation of Porphyromonas gingivalis at 37°C for 74 h. The antifungal activity of PTMCs against Candida albicans, in a pH 5.5 acidic environment was determined in a plate assay. Results Flexural strength decreased with increasing PTMC concentration from 97.58 ± 4.79 MPa for the DBR alone to 53.66 ± 2.46 MPa for DBR containing 5.0% PTMC. No release of phytoncide from the PTMCs in the DBR was detected at pH 7.4. The PTMCs had a minimal inhibitory concentration of 2.6% (wt/vol) against C. albicans at pH 5.5. Conclusions PTMCs can be added to DBR 2.5% (wt/wt) without adversely affecting flexural strength. PTMCs released the antimicrobial agent at pH 5.5 at concentrations sufficient to inhibit the growth of the C. albicans.
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publishDate 2018
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spelling iium-644422019-01-31T06:38:10Z http://irep.iium.edu.my/64442/ Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin Steve, An Judge, Roy B Wong, Rebecca H Arzmi, Mohd Hafiz Palamara, J E Dashper, Stuart QR Microbiology RK Dentistry Background This study aimed to fabricate a denture base resin (DBR) containing phytoncide microcapsules (PTMCs) and determine the mechanical properties of the resin and antifungal activity. Methods Fifty‐four heat cured rectangular DBR specimens (64 x 10 x 3.3 ± 0.2 mm) containing nine concentrations of PTMC between 0 ‐ 5% (wt/wt) were fabricated and subjected to a three‐point bending test. A phytoncide release bioassay was developed using DBR containing 0% and 2.5% PTMCs (wt/wt) in a 24 well‐plate assay with incubation of Porphyromonas gingivalis at 37°C for 74 h. The antifungal activity of PTMCs against Candida albicans, in a pH 5.5 acidic environment was determined in a plate assay. Results Flexural strength decreased with increasing PTMC concentration from 97.58 ± 4.79 MPa for the DBR alone to 53.66 ± 2.46 MPa for DBR containing 5.0% PTMC. No release of phytoncide from the PTMCs in the DBR was detected at pH 7.4. The PTMCs had a minimal inhibitory concentration of 2.6% (wt/vol) against C. albicans at pH 5.5. Conclusions PTMCs can be added to DBR 2.5% (wt/wt) without adversely affecting flexural strength. PTMCs released the antimicrobial agent at pH 5.5 at concentrations sufficient to inhibit the growth of the C. albicans. Wiley Online Library 2018-06 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/64442/7/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/64442/19/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial.pdf application/pdf en http://irep.iium.edu.my/64442/20/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial_WOS.pdf Steve, An and Judge, Roy B and Wong, Rebecca H and Arzmi, Mohd Hafiz and Palamara, J E and Dashper, Stuart (2018) Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin. Australian Dental Journal, 63 (3). pp. 302-311. ISSN 1834-7819 https://onlinelibrary.wiley.com/doi/pdf/10.1111/adj.12640 10.1111/adj.12640
spellingShingle QR Microbiology
RK Dentistry
Steve, An
Judge, Roy B
Wong, Rebecca H
Arzmi, Mohd Hafiz
Palamara, J E
Dashper, Stuart
Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin
title Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin
title_full Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin
title_fullStr Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin
title_full_unstemmed Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin
title_short Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin
title_sort incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin
topic QR Microbiology
RK Dentistry
url http://irep.iium.edu.my/64442/
http://irep.iium.edu.my/64442/
http://irep.iium.edu.my/64442/
http://irep.iium.edu.my/64442/7/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial_SCOPUS.pdf
http://irep.iium.edu.my/64442/19/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial.pdf
http://irep.iium.edu.my/64442/20/64442_Incorporation%20of%20the%20microencapsulated%20antimicrobial_WOS.pdf